miR-144 inhibits growth and metastasis of cervical cancer cells by targeting VEGFA and VEGFC

Tao, Pingping; Wen, Hao; Yang, Bo; Ai, Zhang; Wu, Xiaohua; Li, Qing

doi:10.3892/etm.2017.5392

Cited by 25 publications

(36 citation statements)

References 44 publications

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“…In contrast, miR‐144 is aberrantly downregulated and acts as a tumour suppressor in several types of tumours, such as hepatocellular carcinoma (He et al., ), gastric cancer (Liu et al., ) and breast cancer (Yin, Cai, Meng, Sui, & Jiang, ). Interestingly, miR‐144 was reported to be significantly downregulated in cervical cancer tissues and cell lines, and overexpression of miR‐144 could inhibit cell proliferation and invasion in cervical cancer cells (Tao et al., ). In our study, a dual luciferase reporter assay demonstrated that miR‐144 repressed the expression of ITGA6 by directly targeting to its 3′‐UTR.…”

Section: Discussionmentioning

confidence: 99%

“…As a class of endogenous non-coding small RNAs with 21-23 nucleotides, miRNAs, have been shown to regulate posttranscriptional eukaryotic gene expression effectively (Yang et al, 2015). Among these miRNAs, miR-144 was found to be significantly downregulated in cervical cancer tissues, and ectopic expression of miR-144 could inhibit cervical cancer cell migration and invasion by targeting vascular endothelial growth factor A (VEGFA) and VEGFC (Tao et al, 2018). Integrin 6 (ITGA6) is a vital transmembrane protein and acts as an oncogene in various cancers (Raab-Westphal, Marshall, & Goodman, 2017).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Long non‐coding RNA activated by transforming growth factor‐β promotes proliferation and invasion of cervical cancer cells by regulating the miR‐144/ITGA6 axis

Zhu

Yang

et al. 2019

Experimental Physiology

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New Findings What is the central question of this study?This study was designed to investigate the molecular mechanism and biological roles of long non‐coding RNA activated by transforming growth factor‐β (lncRNA ATB) in the progression of cervical cancer. What is the main finding and its importance?Our study provided new insight into the cross‐talk between lncRNA ATB, miR‐144 and ITGA6, shedding light on the therapy for cervical cancer. Abstract The present study was designed to investigate the molecular mechanism and biological roles of long non‐coding RNA activated by transforming growth factor‐β (lncRNA ATB) in the progression of cervical cancer. The expression levels of lncRNA ATB, miR‐144 and integrin α6 (ITGA6) were detected in human cervical cancer cell lines using quantitative real‐time PCR and western blotting. Cell viability was quantified by MTT assay at 12, 24, 36, 48 and 72 h after transfection, and cell invasion was determined by the Transwell migration assay. The association among lncRNA ATB, miR‐144 and ITGA6 was disclosed by a dual‐luciferase reporter assay. We found that lncRNA ATB was highly expressed in human cervical cancer cell lines. Further investigation indicated that lncRNA ATB functioned as a competitive endogenous RNA (ceRNA) for miR‐144 to promote cervical cancer cell proliferation and invasion. We demonstrated that ITGA6 was a direct target of miR‐144, and lncRNA ATB facilitated the proliferation and invasion of cervical cancer cells via the miR‐144/ITGA5 axis. In conclusion, the lncRNA ATB/miR‐144/ITGA6 axis might be a promising therapeutic target for cervical cancer.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Long non‐coding RNA activated by transforming growth factor‐β promotes proliferation and invasion of cervical cancer cells by regulating the miR‐144/ITGA6 axis

Zhu

Yang

et al. 2019

Experimental Physiology

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“…MiR-144 is significantly decreased in CC cells when compared to normal cervical cells. MiR-144 suppresses the proliferation and angiogenesis of tumor cells by targeting VEGFA and VEGFC [109]. Furthermore, miR-144 overcomes resistance to cisplatin (CDDP) through targeting of LIM homeobox 2 (Lhx2), leading to apoptosis and inhibiting invasion in tumor cells [110].…”

Section: Mir-144 In Cervical Cancermentioning

confidence: 99%

MiR-144: A New Possible Therapeutic Target and Diagnostic/Prognostic Tool in Cancers

Kooshkaki

Rezaei

Rahmati

et al. 2020

IJMS

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MicroRNAs (miRNAs) are small and non-coding RNAs that display aberrant expression in the tissue and plasma of cancer patients when tested in comparison to healthy individuals. In past decades, research data proposed that miRNAs could be diagnostic and prognostic biomarkers in cancer patients. It has been confirmed that miRNAs can act either as oncogenes by silencing tumor inhibitors or as tumor suppressors by targeting oncoproteins. MiR-144s are located in the chromosomal region 17q11.2, which is subject to significant damage in many types of cancers. In this review, we assess the involvement of miR-144s in several cancer types by illustrating the possible target genes that are related to each cancer, and we also briefly describe the clinical applications of miR-144s as a diagnostic and prognostic tool in cancers.Int. J. Mol. Sci. 2020, 21, 2578 2 of 23 of a mRNA molecule [3]. MiRNAs indicate a new perspective in the study of cancers. More than 50% of miRNA genes were discovered to be located within the genomic regions that are involved in cancers, suggesting their role in human cancer pathogenesis. In pathological conditions, MiRNAs can negatively regulate gene expression and they are associated with tumor growth, apoptosis, invasion, and metastasis. In the past, several studies have indicated the ability of miRNAs in the inhibition of tumors as a critical option for the improvement of cancer therapy [4,5]. Tumor activator miRNAs, called oncomiRs, are overexpressed in various cancers. On the contrary, suppressive tumor miRNAs are underexpressed [6][7][8]. Among several miRNAs assessed, miR-144s seem to have a role in several cancers. These miRNAs are located in the chromosomal region 17q11.2, being significantly destroyed in many types of cancers. The predicted stem-loop structure of miR-144s recognized by the miRBase (http://mirbase.org/) is shown in Figure 1A. The miR-144 hairpin gives rise to the "guide strand" miR-144-5p and the sister "passenger" strand miR-144-3p ( Figure 1B).

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“…Cancer cells overexpress COX-2 and prostaglandins that reduce cancer cell apoptosis and induce angiogenesis via VEGF (Fosslien, 2001b). Recently, miR-144 acts as a tumor suppressor in the proliferation and metastasis of cervical cancer cells by directly targeting VEGF (Tao et al, 2018). The present study identified with qPCR and western blot analyses that the downregulation of miR-144 may contribute to COX-2 and VEGF expression in ovarian cancers.…”

Section: Discussionmentioning

confidence: 58%

Suppression of MicroRNA-144 Promotes CXCR4 and CXCL12 Expression and Downregulates Apoptosis in Ovarian Cancer Cells

Turut

Acıdereli

Çevik

2020

Preprint

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MicroRNAs are important regulators in the growth and metastasis of ovarian cancers. Many assays were established to identify the role of miR-144-3p in ovarian cancer cells and its interaction with COX-2 and chemokines (CXCR4 and CXCL12). The ovarian cancer cells (OVCAR-3 and SKOV-3) were transfected with Anti-miR-144 to downregulate the miR-144-3p and cultured for 36 h. We herein examined the cell viability, colony formation, cell migration, COX-2 reporter activity, the protein expressions of CXCR4, CXCL12, COX-2, VEGF, Caspase-3, BAX and Bcl-2. We have observed that the suppression of miR-144-3p significantly increased the cell proliferation and migration and decreased the apoptosis. Moreover, the downregulation of miR-144-3p markedly increased the COX-2, CXCR4, CXCL12 and VEGF expression in OVCAR-3 and SKOV-3 ovarian cancer cells. In conclusion, miR-144-3p may play important roles in the regulation of chemokine receptor CXCR4 and its ligand CXCL12 in the progressive ovarian tumors expressing COX2. These data suggests that miR-144 has the novel therapeutic targets for the cancer therapy and cancer prevention.

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miR-144 inhibits growth and metastasis of cervical cancer cells by targeting VEGFA and VEGFC

Abstract: Abstract. MicroRNAs (miRs) are aberrantly expressed in various cancer types and have critical roles in their genesis and progression. miR-144 has been identified to be involved in the development of hepatocellular carcinoma and rectal cancer.

Cited by 25 publications

References 44 publications

Long non‐coding RNA activated by transforming growth factor‐β promotes proliferation and invasion of cervical cancer cells by regulating the miR‐144/ITGA6 axis

Long non‐coding RNA activated by transforming growth factor‐β promotes proliferation and invasion of cervical cancer cells by regulating the miR‐144/ITGA6 axis

MiR-144: A New Possible Therapeutic Target and Diagnostic/Prognostic Tool in Cancers

Suppression of MicroRNA-144 Promotes CXCR4 and CXCL12 Expression and Downregulates Apoptosis in Ovarian Cancer Cells

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