MiR-137 affects melanin synthesis in mouse melanocyte by repressing the expression ofc-KitandTyrp2inSCF/c-Kitsignaling pathway

Jiang, Shan; Yu, Xiuju; Dong, Chunhong

doi:10.1080/09168451.2016.1200455

Cited by 17 publications

(14 citation statements)

References 43 publications

(33 reference statements)

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“…Similarly, in mouse skin melanocytes, we found that miR-21a-5p targets SOX5 through the MITF pathway, and miR-27a-3p regulates Wnt3a to modulate melanogenesis [23,24]. Transgenic mice overexpressing miR-137 can change coat color, and miR-137 could inhibit the pigmentation of mouse skin melanocytes by the SCF/c-Kit pathway [25]. In contrast, miR-488 could degrade POMC mRNA in keratinocytes to modulate mice coat color by the α-MSH/ MC1R pathway [26].…”

Section: Introductionmentioning

confidence: 68%

Exosomal miRNA derived from keratinocytes regulates pigmentation in melanocytes

Liu

Gao

et al. 2019

Journal of Dermatological Science

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Background: Pigmentation is controlled by complex mechanisms. Evidence suggests that miRNAs can regulate pigmentation. However, the mechanism has not been fully elucidated. Objective In this study, we revealed a novel mechanism that regulates pigmentation involving exosomes, miRNAs and the crosstalk between keratinocytes and melanocytes. Methods: The expression and localization of exosome specific marker TSG101 in keratinocytes and melanocytes; Changes of melanin content in melanocytes after co-culture of exosome and melanocytes; Expression changes of target gene TYR and its related genes and inhibitory effect of miR-330-5p on pigmentation were studied by using various molecular biological techniques. Results: In this experiment, we used miR-330-5p in keratinocytes to verify the effect of keratinocyte derived exosome on melanocyte pigmentation. First, we found that keratinocytes secrete exosomes carrying miR-330-5p; moreover, greater miR-330-5p expression was found in exosomes derived from keratinocytes that overexpressed miR-330-5p. Second, we found that exosomes derived from keratinocytes with overexpression of miR-330-5p caused a significant increase in miR-330-5p in melanocytes. Finally, exosomes derived from keratinocytes that overexpressed miR-330-5p induced a significant decrease in the production of melanin and expression of TYR in melanocytes. Meanwhile, we overexpressed miR-330-5p in melanocytes, which also proved the inhibitory effect of miR-330-5p on pigmentation. Conclusion: These findings suggest that keratinocytes crosstalk with melanocytes in the epidermal melanin unit via exosomal miRNAs. These studies reveal an important role of exosomes in melanocyte pigmentation, which opens a new pathway of melanogenesis.

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Section: Introductionmentioning

confidence: 68%

Exosomal miRNA derived from keratinocytes regulates pigmentation in melanocytes

Liu

Gao

et al. 2019

Journal of Dermatological Science

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“…Indeed, miR‐137 was initially shown as a regulator of MITF in human melanoma cell lines . In a second study, the overexpression of miR‐137 was followed by the downregulation of KIT and TYRP2 genes and reduction of melanin content in mouse skin melanocytes . In this latter study, KIT was evidenced as a direct target of miR‐137.…”

Section: Non‐coding Rnas and The Regulation Of Melanogenesismentioning

confidence: 90%

Intrinsic and extrinsic regulation of human skin melanogenesis and pigmentation

Serre

Busuttil

Botto

2018

Intern J of Cosmetic Sci

179

187

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In human skin, melanogenesis is a tightly regulated process. Indeed, several extracellular signals are transduced via dedicated signalling pathways and mostly converge to MITF, a transcription factor integrating upstream signalling and regulating downstream genes involved in the various inherent mechanisms modulating melanogenesis. The synthesis of melanin pigments occurs in melanocytes inside melanosomes where melanogenic enzymes (tyrosinase and related proteins) are addressed with the help of specific protein complexes. The melanosomes loaded with melanin are then transferred to keratinocytes. A more elaborate level of melanogenesis regulation comes into play via the action of non-coding RNAs (microRNAs, lncRNAs). Besides this canonical regulation, melanogenesis can also be modulated by other non-specific intrinsic pathways (hormonal environment, inflammation) and by extrinsic factors (solar irradiation such as ultraviolet irradiation, environmental pollution). We developed a bioinformatic interaction network gathering the multiple aspects of melanogenesis and skin pigmentation as a resource to better understand and study skin pigmentation biology.

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“…Of note, most of the miR-encoding DEGs (11 out of 13) had the other DEGs as their predicted targets (Table III), the majority of which were significantly decreased in grey compared with black hair follicles. Furthermore, among the miR-targeted DEGs, two genes (syndecan binding protein and KIT) are involved in pigment-associated pathways (26,27).…”

Section: Resultsmentioning

confidence: 99%

Global downregulation of pigmentation‑associated genes in human premature hair graying

et al. 2019

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Premature hair graying, or canities, is a complex multi-factorial process with negative effects on affected individuals. The aim of the present study was to investigate the possible underlying mechanisms of premature hair graying at the genetic level. A total of 5 unrelated Han Chinese individuals presenting with premature hair graying (25–40 years old, with >1% hair affected) were enrolled in the present study. RNA sequencing was performed to identify gene expression changes between the follicular cells of grey and black hair from the cohort. A total of 127 differentially expressed genes (DEGs) were identified. These DEGs were overrepresented in categories associated with the pigmentation pathway, with a decreased expression of key genes responsible for melanin synthesis. Of note, the decreased expression of certain transcription factors and the increased expression of certain precursor microRNAs observed may explain for the downregulation of certain other DEGs, which were identified as their targets via Starbase v2 and Integrated Motif Activity Response Analysis. The DEGs were also enriched in terms associated with the nervous system, indicating that neural disturbances may also have certain roles in premature hair graying. Of note, five of the downregulated DEGs were associated with aging according to the JenAge Aging Factor Database. To the best of our knowledge, the present study was the first genome-wide survey of the gene expression profile associated with premature hair graying. Dysfunction of the melanin biosynthesis pathway is probably the direct cause of hair graying and the present results provide valuable clues for further functional and mechanistic investigation.

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MiR-137 affects melanin synthesis in mouse melanocyte by repressing the expression ofc-KitandTyrp2inSCF/c-Kitsignaling pathway

Cited by 17 publications

References 43 publications

Exosomal miRNA derived from keratinocytes regulates pigmentation in melanocytes

Exosomal miRNA derived from keratinocytes regulates pigmentation in melanocytes

Intrinsic and extrinsic regulation of human skin melanogenesis and pigmentation

Global downregulation of pigmentation‑associated genes in human premature hair graying

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