miR-130a acts as a potential diagnostic biomarker and promotes gastric cancer migration, invasion and proliferation by targeting RUNX3

Jiang, Hong; Yu, Wei; Wang, Lulu; Yang, Peng

doi:10.3892/or.2015.4099

Cited by 67 publications

(61 citation statements)

References 41 publications

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“…miRNAs have important roles in regulating a number of cell processes, including cell differentiation, apoptosis and cell cycle progression (5)(6)(7)(8)(9). miRNAs serve as biomarkers in various types of cancer, including GC and prostate cancer (10,11). Although a number of miRNAs have been identified to be dysregulated in GC tissues, the role of miR-1258 in GC has not, to the best of our knowledge, previously been investigated.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-1258: An invasion and metastasis regulator that targets heparanase in gastric cancer

et al. 2017

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Abstract. Numerous microRNAs (miRNAs/miRs) are involved in suppressing or promoting the formation of cancer. However, to the best of our knowledge, the role of miRNA-1258 in gastric cancer (GC) has not previously been investigated. Our previous study demonstrated an increased expression of heparanase (HPSE) in GC tissues and HPSE-facilitated invasion and metastasis of GC cells. Consequently, in the present study, the function of miR-1258 in the invasion and metastasis of GC cells was investigated to determine whether miR-1258 is associated with GC through HPSE. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to determine miR-1258 expression in GC cell lines and tissues. A Transwell cell invasion assay and an MTT proliferation assay were used to investigate the effects of miR-1258 on the invasion and proliferation of one of the cell lines, SGC-7901 cells, in vitro. The effect of miR-1258 on SGC-7901 cell metastasis was investigated using an in vivo tumor metastasis formation assay. Western blot analysis and RT-qPCR were used to investigate whether HPSE was a target of miR-1258 in GC. The expression of miR-1258 was significantly downregulated in 3 GC cell lines and 116 GC tissues compared with controls (all P<0.001). An association was identified between decreased miR-1258 expression level and increased age (P=0.042), advanced pathological tumor stage (P=0.027) and positive lymphatic vessel invasion (P=0.044) in patients with GC. Furthermore, the upregulation of miR-1258 expression suppressed SGC-7901 cell invasion in vitro (P<0.001) and inhibited SGC-7901 cell metastasis in vivo (P=0.016). The western blot analysis and RT-qPCR results indicated that miR-1258 downregulated the expression of HPSE at the translational level. The results of the present study indicate that miR-1258 acts as a tumor suppressor to inhibit invasion and metastasis by targeting HPSE. Therefore, miR-1258 may serve as a novel biomarker and therapeutic target in the treatment of GC.

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Section: Introductionmentioning

confidence: 99%

MicroRNA-1258: An invasion and metastasis regulator that targets heparanase in gastric cancer

et al. 2017

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“…Previous studies have suggested that miRNAs may be used as molecular biomarkers in the diagnosis and prognosis of GC (10)(11)(12). Oncogenic miRNAs, which are generally upregulated in GC, exert their function by silencing the expression of genes that inhibit the cell cycle and/or genes associated with apoptotic pathways (13)(14)(15). By contrast, miRNAs that are downregulated act as tumor suppressors during GC progression.…”

Section: Introductionmentioning

confidence: 99%

“…By contrast, miRNAs that are downregulated act as tumor suppressors during GC progression. These miRNAs generally exert their function via anti-proliferative and pro-apoptotic associated genes (14)(15)(16). Additionally, decreased levels of miR-144 in GC tissue may be associated with poor patient prognosis and it was demonstrated that the reintroduction of miR-144 increased the susceptibility of cancer cells to chemotherapy (17,18).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-144 functions as a tumor suppressor in gastric cancer by targeting cyclooxygenase-2

Yao

Wang

et al. 2018

Exp Ther Med

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Abstract. Gastric cancer (GC) poses a serious public health threat and the 5-year survival rate of patients with GC is low. MicroRNAs (miRNAs/miRs) may serve oncogenic or tumor suppressor functions during tumorigenesis by regulating cell proliferation, apoptosis, migration and invasion and it has been demonstrated that they may be dysregulated in various types of cancer. The present study demonstrated that miR-144 and GATA4 were downregulated in GC tissues and cell lines and suggested that this may be due to hypermethylation. Additionally, miR-144 and GATA4 had synergistic effects on GC cells by repressing cell proliferation and inducing cell cycle arrest and apoptosis. The results of bioinformatics and a luciferase reporter assay indicated that cyclooxygenase-2 (COX-2) is a direct target of miR-144 and that miR-144 negatively regulated the expression of COX-2, which inhibits the viability of GC cells. GATA4 also induced a similar effect on COX-2. Taken together, the results of the present study may improve understanding of the underlying mechanism of miR-144 and GATA4 in GC.

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“…Studies indicate that miR-130a is associated with lung cancer [50], gastric cancer [51,52], ovarian cancer [53][54][55][56][57], cervical cancer [58,59], and prostate cancer [60]. Recently, deep sequencing technology was used to identify differentially expressed host miRNAs in chickens [28,34,35,61,62].…”

Section: Discussionmentioning

confidence: 99%

“…A previous study showed that miR-130a modulates vascular smooth muscle cell proliferation under hypertension [63]. In addition, miR-130a promots cell proliferation and migration by targeting RUNX3 (runt-related transcription factor 3) in gastric cancer [51]. In human breast cancer, miR-130a was found to inhibit cell proliferation and migration by targeting RAB5A (member RAS oncogene family) [64].…”

Section: Discussionmentioning

confidence: 99%

Chicken gga-miR-130a targets HOXA3 and MDFIC and inhibits Marek’s disease lymphoma cell proliferation and migration

Han

Lian

et al. 2016

Mol Biol Rep

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Marek's disease (MD) is an infectious disease of chickens caused by MD virus (MDV), which is a herpesvirus that initiates tumor formation. Studies have indicated that microRNAs (miRNAs) are linked with the development of cancers or tumors. Previously, gga-miR-130a was discovered downregulated in MDV-infected tissues. Here, we aimed to explore the further function of gga-miR-130a in MD. The expression of gga-miR-130a in MDV-infected and uninfected spleens was detected by quantitative real-time PCR (qRT-PCR). Subsequently, proliferation and migration assays of MDV-transformed lymphoid cells (MSB1) were carried out by transfecting gga-miR-130a. The target genes of gga-miR-130a were predicted using TargetScan and miRDB and clustered through Gene Ontology analysis. The target genes were validated by western blot, qRT-PCR, and a dual luciferase reporter assay. Our results show that the expression of gga-miR-130a was reduced in MDV-infected spleens. Gga-miR-130a showed an inhibitory effect on MSB1 cell proliferation and migration. Two target genes, homeobox A3 (HOXA3) and MyoD family inhibitor domain containing (MDFIC), were predicted and clustered to cell proliferation. Results indicate that gga-miR-130a regulates HOXA3 and MDFIC at the protein level but not at the mRNA level. Moreover, the gga-miR-130a binding sites of two target genes have been confirmed. We conclude that gga-miR-130a can arrest MSB1 cell proliferation and migration, and target HOXA3 and MDFIC, which are both involved in the regulation of cell proliferation. Collectively, gga-miR-130a plays a critical role in the tumorigenesis associated with chicken MD.

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miR-130a acts as a potential diagnostic biomarker and promotes gastric cancer migration, invasion and proliferation by targeting RUNX3

Cited by 67 publications

References 41 publications

MicroRNA-1258: An invasion and metastasis regulator that targets heparanase in gastric cancer

MicroRNA-1258: An invasion and metastasis regulator that targets heparanase in gastric cancer

MicroRNA-144 functions as a tumor suppressor in gastric cancer by targeting cyclooxygenase-2

Chicken gga-miR-130a targets HOXA3 and MDFIC and inhibits Marek’s disease lymphoma cell proliferation and migration

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