miR-124a expression contributes to the monophasic pattern of insulin secretion in islets from pregnant rats submitted to a low-protein diet

Siqueira, Kariny Cassia de; Lima, Faena Moura de; Lima, Fernanda Souza; Taki, Marina Satie; Cunha, Clarissa Felfili da; Reis, Silvia Regina de Lima; Camargo, Rafael Ludemann; Batista, Thiago M.; Vanzela, Emerielle C.; Nardelli, Tarlliza Romanna; Carneiro, Everardo M.; Bordin, Silvana; Ignácio-Souza, Letícia Martins; Latorraca, Márcia Queiroz

doi:10.1007/s00394-017-1425-z

Cited by 11 publications

(10 citation statements)

References 40 publications

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“…The expected biphasic secretion reported in normal pregnancy (Sorenson & Brelje, ) and observed in our control non‐pregnant pregnant rats was not verified in recovered and control pregnant rats. However, the pattern of insulin secretion exhibited by our control pregnant rats was also verified previously (de Siqueira et al., ). Interestingly, the kinetics of insulin secretion were not modified by pregnancy in the recovered and protein‐deficient groups.…”

Section: Discussionsupporting

confidence: 91%

“…Short‐term protein restriction during pregnancy (from the first to the 15th day) produced higher basal insulin secretion, and the maximal insulin secretion was obtained in a physiological glucose range (Souza et al., ). This experimental model showed loss of biphasic insulin secretion that appeared to result from changes in exocytotic elements that are influenced either directly or indirectly by miR‐124a (de Siqueira et al., ). In contrast, in islets from pregnant rats that were subjected to prolonged protein restriction (from intrauterine life to adult life and pregnancy) there was reduced glucose responsiveness but similar glucose sensitivity.…”

Section: Introductionmentioning

confidence: 99%

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Protein restriction in early life increases intracellular calcium and insulin secretion, but does not alter expression of SNARE proteins during pregnancy

Marín

Reis

Ramalho

et al. 2019

Experimental Physiology

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New Findings What is the central question of this study?Does protein restriction in early life modify glucose‐induced insulin secretion by altering [Ca2+]i and the expression of SNARE proteins in pancreatic islets from pregnant rats? What is the main finding and its importance?Protein restriction in early life increased the first phase of glucose‐induced insulin secretion and [Ca2+]i without altering the expression of SNARE proteins during pregnancy. This finding contributes to our understanding of the mechanisms of altered insulin secretion and might provide new perspectives for the development of therapeutic tools for gestational diabetes. Abstract We investigated the kinetics of glucose‐induced insulin secretion and their relationship with [Ca2+]i and the expression of protein from exocytotic machinery in islets from recovered pregnant and long‐term protein‐deficient pregnant rats. Isolated islets were evaluated from control‐fed pregnant (CP), protein‐deficient pregnant (DP), control‐fed non‐pregnant (CNP) and protein‐deficient non‐pregnant (DNP) female adult rats, and from protein‐deficient pregnant (RP) and non‐pregnant (RNP) rats that were recovered after weaning. The insulin responses to glucose during the first phase of secretion were higher in RP than in CP groups, and both were higher than in the DP group. Islets from RP rats displayed a rapid increase in insulin release (first phase), followed by a plateau that was maintained thereafter. The [Ca2+]i in islets from the protein‐deficient groups was lower than in the control groups, and both were lower than in the RP and RNP groups. SNAP‐25 was increased in islets from pregnant rats independently of their nutritional status, and the syntaxin‐1A content was reduced in islets from the RP rats compared with the RNP rats. The VAMP2 content was similar among the groups. Thus, protein restriction during intrauterine life and lactation increased insulin secretion during pregnancy, attributable, in part, to increased [Ca2+]i, and independent of an alteration of expression of SNARE proteins.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Protein restriction in early life increases intracellular calcium and insulin secretion, but does not alter expression of SNARE proteins during pregnancy

Marín

Reis

Ramalho

et al. 2019

Experimental Physiology

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“…Although our results showed no damage in low-protein dams, it is known that a restriction of protein during pregnancy may induce changes in other pathways, such as glucoses metabolism and insulin secretion ( 37 , 38 ). A decrease in (Ca 2+ )i, as well as changes in gene expression in pancreatic islets ( 39 ) can explain the decreased insulin secretion in malnourished animals. A study also demonstrated that physical training before and during pregnancy attenuated the effects of a low-protein diet on the secretion of insulin ( 17 ).…”

Section: Discussionmentioning

confidence: 99%

Low-protein diet does not alter reproductive, biochemical, and hematological parameters in pregnant Wistar rats

Barros

Andrade

Barros

et al. 2018

Braz J Med Biol Res

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The aim of this study was to investigate the reproductive, biochemical, and hematological outcomes of pregnant rats exposed to protein restriction. Wistar rat dams were fed a control normal-protein (NP, 17% protein, n=8) or a low-protein (LP, 8% protein, n=14) diet from the 1st to the 20th day of pregnancy. On the 20th day, the clinical signs of toxicity were evaluated. The pregnant rats were then anesthetized and blood samples were collected for biochemical-hematological analyses, and laparotomy was performed to evaluate reproductive parameters. No sign of toxicity, or differences (P>0.05) in body weight gain and biochemical parameters (urea, creatinine, albumin, globulin, and total protein) between NP and LP pregnant dams were observed. Similarly, hematological data, including red blood cell count, white blood cell count, hemoglobin, hematocrit, red blood cell distribution width (coefficient of variation), mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, % lymphocytes, absolute lymphocyte count, platelet count, and mean platelet volume were similar (P>0.05) at the end of pregnancy. Reproductive parameters (the dam-offspring relationship, ovary mass, placenta mass, number of corpora lutea, implantation index, resorption index, and the pre- and post-implantation loss rates) were also not different (P>0.05) between NP and LP pregnant dams. The present data showed that a protein-restricted diet during pregnancy did not alter reproductive, biochemical, and hematological parameters and seems not to have any toxic effect on pregnant Wistar rats.

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“…This effect may be partly explained by the increased β-cell and liver glucose transporter GLUT2 in the membrane of cultured neonatal rat islets. [25]…”

Section: Discussionmentioning

confidence: 99%

Metabolic profile and prolactin serum levels in men with type 2 diabetes mellitus: Old-new rubric

Al-Nami

Al-Kuraishy

Al-Gareeb

et al. 2019

Int J Crit Illn Inj Sci

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Background:Prolactin (PRL) is involved in the regulation of glucose metabolism since high PRL serum levels are associated with low incidence of type 2 diabetes mellitus (T2DM). Therefore, the aim of the present study was to assess the metabolic effects of PRL on glucose homeostasis in men with T2DM.Methods:Eighty male patients with T2DM compared with 25 male healthy controls matched with patients for age and weight were divided into four groups: Group (A): patients with T2DM on metformin (n = 29), Group (B): patients with T2DM on glyburide (n = 30), Group (C): patients with T2DM on glyburide plus metformin (n = 21), and Group (D): healthy male subjects as control (n = 25). Body mass index (BMI) and blood pressure measurements were determined. Fasting blood glucose (FBG), glycated hemoglobin, total cholesterol, triglyceride (TG), high-density lipoprotein, low-density lipoprotein, atherogenic index, fasting serum insulin, insulin resistance (IR), and β-cell function of the pancreas were determined by homeostatic model assessment-2 (HOMA-IR). Furthermore, C-reactive protein and PRL serum level were determined in patients with T2DM and healthy control men.Results:BMI of T2DM patients was higher as compared with control (P = 0.003). Combination therapy (glyburide plus metformin) in patients with T2DM showed better effect on most of glycemic indices and lipid profile than glyburide or metformin monotherapy (P < 0.05). PRL serum level was higher in patients with T2DM as compared with control (P = 0.001). PRL serum level was high in glyburide-treated patients as compared with metformin-treated patients (P = 0.002).Conclusion:This study concludes that elevated PRL serum level in patients with T2DM is associated with diabetic complications. Diabetic pharmacotherapy mainly metformin reduced PRL serum level in patients with T2DM through amelioration of IR.

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miR-124a expression contributes to the monophasic pattern of insulin secretion in islets from pregnant rats submitted to a low-protein diet

Abstract: Loss of biphasic insulin secretion in islets from LPP rats appears to have resulted from reduced [Ca] due, at least in part, to Kir6.2 underexpression and from the changes in exocytotic elements that are influenced either directly or indirectly by miR-124a.

Cited by 11 publications

References 40 publications

Protein restriction in early life increases intracellular calcium and insulin secretion, but does not alter expression of SNARE proteins during pregnancy

Protein restriction in early life increases intracellular calcium and insulin secretion, but does not alter expression of SNARE proteins during pregnancy

Low-protein diet does not alter reproductive, biochemical, and hematological parameters in pregnant Wistar rats

Metabolic profile and prolactin serum levels in men with type 2 diabetes mellitus: Old-new rubric

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