Miniaturized most probable number for the enumeration of Salmonella sp in artificially contaminated chicken meat

Colla, F. L.; Mion, Ligiani; Parizotto, Luana; Rodrigues, L. B.; Pilotto, Fernando; Dickel, EL; Nascimento, Vladimir Pinheiro do; Santos, Luciana R.

doi:10.1590/s1516-635x2014000100006

Cited by 3 publications

(7 citation statements)

References 14 publications

(16 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Fravalo et al (2003) propuseram uma metodologia de mNMP utilizando o MSRV para seleção de salmonelas móveis e posterior desenvolvimento em meios cromogênicos enquanto Pavic et al Os resultados do presente trabalho, que utilizou a metodologia de Fravalo et al (2003) adaptada por Colla et al (2014), podem ser inicialmente atribuídos a heterogeneidade das amostras, em concordância com Skjerve & Olsvik (1991), ao citar que a matriz afeta a sensibilidade e a especificidade de um protocolo de isolamento e representaria ambientes críticos avícolas, que variam de amostras com baixa umidade e altamente úmidas (carcaças e água do tanque da escaldagem) até ambientes altamente competitivos (cecos e fezes) (USDA-FSIS 2008). Também o limite de detecção do mNMP (0,13 NMP/mL, dados não mostrados) ou os volumes diminutos utilizados no pré-enriquecimento e enriquecimento seletivo, em comparação com a microbiologia convencional, podem ter influenciado negativamente o isolamento e quantificação das amostras.…”

Section: Resultsunclassified

“…nas amostras naturalmente contaminadas foi utilizado o método do mNMP proposto por Fravalo et al (2003) e adaptado por Colla et al (2014), que contém as mesmas fases da microbiologia convencional mas executadas em placas de 24 poços com capacidade para 2,5mL. Previamente ao experimento foram realizados ensaios para determinar o limite de detecção do mNMP em amostras de carne de frango artificialmente contaminadas com Salmonella Enteritidis ATCC 13076, que apontaram um limite de 0,13 NMP/mL.…”

Section: Quantificação De Salmonella Spp Por Número Mais Provável MIunclassified

“…em diferentes pontos da tecnologia de abate de frangos de corte bem como relatar os sorovares isolados nos pontos positivos para a bactéria. Colla et al (2014).…”

Section: Introductionunclassified

See 2 more Smart Citations

Número mais provável miniaturizado e microbiologia convencional para isolamento de Salmonella spp. em abatedouros de frangos de corte

et al. 2015

View full text Add to dashboard Cite

ABSTRACT.-Santos L.A., Mion L., Marotzki M., Parizotto L., Rodrigues L.B., Nascimento V. Poultry products can be important modes of transmission of Salmonella spp. to humans and, among several parameters used to determine food quality, microbiological characteristics play an essential role. The aim of this study was to determine and quantify Salmonella spp. at broiler slaughtering facilities. This was done by conventional microbiology and by the miniaturized most probable number (mMPN) methods. Three federally-inspected slaughterhouses were visited, where samples were collected in triplicate from six sites: reception of live birds (cloacal swabs and sponge samples from transport cages before and after sanitation) and carcass processing (after pre-chiller, after dripping, and before primary packaging and refrigeration at -12 o C for 24h), totaling 108 samples. Three of the six surveyed sites and two of the three slaughterhouses were contaminated with Salmonella spp., showing an infection rate of 5.5% independently of the method used, and revealing that transport cages were contaminated after sanitation. No correlation could be established between the results of conventional microbiology and mMPN methods, and contamination along the slaughtering line could not quantified. This indicates the importance of combining qualitative and quantitative methods for the enumeration of Salmonella when detection rates are lower than the proposed mMPN limit (0.13 MPN/mL). Typhimurium, Panama, Lexington and Rissen, which are paratyphoid organisms and are potentially infectious to humans, were identified. However, these serovars were isolated at the reception of live birds (from cloacal swabs and from transport cages) rather than from the end products. Given that Salmonella spp. was detected in transport cages after sanitation, it is paramount that automated washing procedures currently used in slaughterhouses be reassessed and adjusted.INDEX TERMS: Salmonella spp., miniaturized most probable number, serovars, poultry slaughterhouses.

show abstract

Section: Resultsunclassified

Section: Quantificação De Salmonella Spp Por Número Mais Provável MIunclassified

See 1 more Smart Citation

Número mais provável miniaturizado e microbiologia convencional para isolamento de Salmonella spp. em abatedouros de frangos de corte

et al. 2015

View full text Add to dashboard Cite

show abstract

“…The carcasses were stored in sealed individual bags, kept under refrigeration and sent to the laboratory, where they were rinsed with 400 mL of BPW 1%. Aliquots of 17.5 mL were obtained from each sample, of which 7.5 mL was submitted to mMPN [5,8] and 10 mL underwent conventional microbiology for Salmonella spp. isolation [10].…”

Section: Sampling and Collectionmentioning

confidence: 99%

“…Quantitative methods, such as the most probable number (MPN), allow estimating bacterial colonization based on statistical probabilities [16]. However, as it employs multiple tube series, the MPN method is time-consuming and financially unrewarding, and therefore impractical for hazard analysis, especially when several samples are analyzed simultaneously [5]. Enumeration methods using miniaturized most probable number (mMPN) with microtiter plates may be appropriate for microbial quantification, as they are quicker, more accurate and less expensive than the conventional MPN method [17].…”

Section: Introductionmentioning

confidence: 99%

Salmonella spp. Isolated by Miniaturized Most Probable Number and Conventional Microbiology in Poultry Slaughterhouses

Mion

Parizotto

Santos

et al. 2016

Acta Scientiae. Vet.

View full text Add to dashboard Cite

Background: Salmonella spp. are frequently isolated from fowls, and their detection in poultry products varies according to the breeding system and the slaughtering process, bringing risks to the consumer and compromising the marketability. The control of Salmonella in poultry slaughterhouses is based on the detection of bacteria, but the quantification of the agent would be important in assessing risk, as well as in obtaining data to determine the capacity of each step of the process to decrease or increase bacterial contamination. The aims of this study were to propose a method for the quantification of Salmonella in poultry slaughterhouses, frequency of isolation and serovars identified.Materials, Methods & Results: Twenty-one broiler flocks from seven federally inspected slaughterhouses in southern Brazil, totaling 1,071 samples, were assessed by miniaturized most probable number (mMPN) and conventional microbiology. The samples were collected in triplicate at 17 points, which included cloacae, transportation cages before and after sanitization, water (scald tank, supply, pre-chiller and chiller), and carcasses (before and after scalding, defeathering, rinsing, evisceration, final rinsing, chilling at 4ºC, and freezing at -12°C for 24 h, 30 and 60 days). Typical Salmonella colonies were submitted to TSI, LIA, SIM, urea, and polyvalent anti-O antiserum tests, and to final identification by Microarray by Check&Trace. Nine of the 1,071 (0.83%) samples analyzed by mMPN and by conventional microbiology were positive for Salmonella and the following serovars were identified: Anatum, Brandenburg, Agona, Tennessee, Bredeney, Schwarzengrund and Infantis.Discussion: This positive rate was lower than that described by other authors, whose rates ranged from 3% and 39% for the isolation of Salmonella spp. from different sources, such as slaughterhouses and retail sales in samples collected in Brazil. The low frequency of isolation of Salmonella in this study can be attributed to the efficiency of control systems used from the field to the slaughterhouse, such as Good Manufacturing Practices (GMP) and Sanitation Standard Operating Procedures (SSOP), which are HACCP requirements. Also, when slaughtering technology actions are properly managed, such as water replacement and temperatures lower than 4ºC in the chiller, the initial contamination by Salmonella spp. can be reduced, with a decline in contamination from 70% to 20%, and with a reduction in the contamination of broiler carcasses after chilling from 15.8% to 3.3%. On the other hand the contamination of carcasses by Salmonella before pre-chilling and in post-chilling might be due to the automated system, inadequate temperatures during chilling, and inappropriate water chlorination in the assessed meat-packing plant. Of the 17 points evaluated, seven were positive for Salmonella, especially the cages after sanitization and frozen carcasses. The contamination by Salmonella spp. in transportation cages after sanitization indicates inefficiency of the automated system as well as possible bacterial resistance to the sanitizers used in SSOP while the isolation in carcasses frozen for 24 h and 60 days demonstrates the thermal resistance of the bacterium to a conservation method widely used in the food industry. In this work, just one of the nine positive samples for Salmonella was identified by conventional methods (CM) and mMPN. The discrepancy between methods can be explained by the heterogeneous distribution of Salmonella and other bacteria in naturally contaminated samples. Samples that were positive in the qualitative test but negative in the mMPN protocol could have had a number of Salmonella below the detection amount.

show abstract

Quick detection and confirmation of microbes in food and water

Franco-Duarte

Kadam²,

Kaushik³

et al. 2023

Present Knowledge in Food Safety

View full text Add to dashboard Cite

Miniaturized most probable number for the enumeration of Salmonella sp in artificially contaminated chicken meat

Cited by 3 publications

References 14 publications

Número mais provável miniaturizado e microbiologia convencional para isolamento de Salmonella spp. em abatedouros de frangos de corte

Número mais provável miniaturizado e microbiologia convencional para isolamento de Salmonella spp. em abatedouros de frangos de corte

Salmonella spp. Isolated by Miniaturized Most Probable Number and Conventional Microbiology in Poultry Slaughterhouses

Quick detection and confirmation of microbes in food and water

Contact Info

Product

Resources

About