Mimicking the liver function in micro-patterned units: Challenges and perspectives in 3D bioprinting

Heydari, Zahra; Pooyan, Paria; Bikmulina, Polina; Pozdnyakov, A A; Fоmin, Victоr; Seydi, Homeyra; Shpichka, Anastasia; Vosough, Massoud

doi:10.1016/j.bprint.2022.e00208

Cited by 12 publications

(10 citation statements)

References 122 publications

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“…The fast but soft gelation of PEG-fibrin conjugates may provide more favorable conditions for ''gentle'' cell types (e.g. hepatocytes) 50,51 that may increase their survivability within a construct.…”

Section: Discussionmentioning

confidence: 99%

PEG-fibrin conjugates: the PEG impact on the polymerization dynamics

et al. 2023

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Section: Discussionmentioning

confidence: 99%

PEG-fibrin conjugates: the PEG impact on the polymerization dynamics

et al. 2023

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“…[76] When fabricating in vitro models of the hepatic environment that aim to consider the contribution of the hepatic vascular network, primary liver sinusoidal endothelial cells are commonly substituted with Human Umbilical Vein Endothelial Cells (HUVECs). [77][78][79] The main reason pushing their exploitation is the preservation of a strong angiogenic activity, leading to their proliferation on lumen-like structures, also in vitro. [80] Liver sinusoids are also studded with Kupffer cells, which are hepatic macrophages.…”

Section: Cells For Liver Bioinksmentioning

confidence: 99%

Toward 3D‐Bioprinted Models of the Liver to Boost Drug Development

Guagliano

Volpini

Briatico‐Vangosa

et al. 2022

Macromolecular Bioscience

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The main problems in drug development are connected to enormous costs related to the paltry success rate. The current situation empowered the development of high-throughput and reliable instruments, in addition to the current golden standards, able to predict the failures in the early preclinical phase. Being hepatotoxicity responsible for the failure of 30% of clinical trials, and the 21% of withdrawal of marketed drugs, the development of complex in vitro models (CIVMs) of liver is currently one of the hottest topics in the field. Among the different fabrication techniques, 3D-bioprinting is emerging as a powerful ally for their production, allowing the manufacture of three-dimensional constructs characterized by computer-controlled and customized geometry, and inter-batches reproducibility. Thanks to these, it is possible to rapidly produce tailored cell-laden constructs, to be cultured within static and dynamic systems, thus reaching a further degree of personalization when designing in vitro models. This review highlights and prioritizes the most recent advances related to the development of CIVMs of the hepatic environment to be specifically applied to pharmaceutical research, with a special focus on 3D-bioprinting, since the liver is primarily involved in the metabolism of drugs.

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“…Several applications of bioprinted liver-on-chips have already been reported [ 78 , 79 ], but there are still various issues concerning the recapitulation of liver tissues due to the complex microenvironment of the latter.…”

Section: Applications Of Bioprinting In Oocmentioning

confidence: 99%

Bioprinting on Organ-on-Chip: Development and Applications

2022

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Organs-on-chips (OoCs) are microfluidic devices that contain bioengineered tissues or parts of natural tissues or organs and can mimic the crucial structures and functions of living organisms. They are designed to control and maintain the cell- and tissue-specific microenvironment while also providing detailed feedback about the activities that are taking place. Bioprinting is an emerging technology for constructing artificial tissues or organ constructs by combining state-of-the-art 3D printing methods with biomaterials. The utilization of 3D bioprinting and cells patterning in OoC technologies reinforces the creation of more complex structures that can imitate the functions of a living organism in a more precise way. Here, we summarize the current 3D bioprinting techniques and we focus on the advantages of 3D bioprinting compared to traditional cell seeding in addition to the methods, materials, and applications of 3D bioprinting in the development of OoC microsystems.

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Mimicking the liver function in micro-patterned units: Challenges and perspectives in 3D bioprinting

Cited by 12 publications

References 122 publications

PEG-fibrin conjugates: the PEG impact on the polymerization dynamics

PEG-fibrin conjugates: the PEG impact on the polymerization dynamics

Toward 3D‐Bioprinted Models of the Liver to Boost Drug Development

Bioprinting on Organ-on-Chip: Development and Applications

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