Mimicking filtration and transport of rotavirus and adenovirus in sand media using DNA-labeled, protein-coated silica nanoparticles

Pang, Liping; Farkas, Kata; Bennett, Grant; Varsani, Arvind; Easingwood, Richard; Tilley, Richard D.; Nowostawska, Urszula; Lin, Shen

doi:10.1016/j.watres.2014.05.055

Cited by 48 publications

(45 citation statements)

References 52 publications

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“…With no exception, all estimated average M r(v) values for hAdV35 were lower than those of MS2 and UX174, which were reported by Syngouna and Chrysikopoulos (2013) under similar experimental conditions. Pang et al (2014) observed lower mass recovery values for hAdV type 41 (VR-930 strain) than MS2. Note that hAdV35 is less negatively charged than coliphages MS2 and UX174 at the experimental conditions of this study.…”

Section: Resultsmentioning

confidence: 79%

“…For example, MS2 has been reported to be less stable than AdV in groundwater at 20°C, while in transport experiments, eluted AdV concentrations were 2-3 orders of magnitude lower than that of MS2 and UX174, with AdV being markedly more retarded (Pang et al 2014). Although hAdVs have been widely recognized as important indicator viruses of human fecal contamination (Pina et al 1998;Bofill-Mas et al 2000), their use in transport experiments has been only recently recorded in very few manuscripts (Davies et al 2006;Horswell et al 2010;Wong et al 2012Wong et al , 2014.…”

Section: Resultsmentioning

confidence: 99%

“…However, relatively less attention has been given to pathogenic viruses (Pancorbo et al 1988;Quanrud et al 2003;Attinti et al 2010), and specifically to adenoviruses as indicator viruses (Davies et al 2006;Horswell et al 2010;Wong et al 2012Wong et al , 2014. Fluorescent polystyrene nanospheres (FPNS) have also been used as virus surrogates in studies of virus transport in porous media, where 70-nm sized silica nanoparticles were covalently coupled with specific proteins and a DNA marker for sensitive detection (Pang et al 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Due to their high prevalence and environmental stability, hAdVs have been included in the U.S. Environmental Protection Agency's contaminant candidate lists (Davies et al 2006;Wong et al 2014). Adenoviruses are more UV light and heat resistant, compared to other enteric viruses (Horswell et al 2010), and remain infectious for at least one year, in groundwater, with their DNA being detectable for as long as 22 months (Pang et al 2014). Quantitative detection of hAdVs as indicators of human fecal contamination is essential because of the frequent reuse of treated wastewater (Fong et al 2010).…”

Section: Introductionmentioning

confidence: 99%

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Transport of Human Adenoviruses in Water Saturated Laboratory Columns

et al. 2015

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Groundwater may be contaminated with infective human enteric viruses from various wastewater discharges, sanitary landfills, septic tanks, agricultural practices, and artificial groundwater recharge. Coliphages have been widely used as surrogates of enteric viruses, because they share many fundamental properties and features. Although a large number of studies focusing on various factors (i.e. pore water solution chemistry, fluid velocity, moisture content, temperature, and grain size) that affect biocolloid (bacteria, viruses) transport have been published over the past two decades, little attention has been given toward human adenoviruses (hAdVs). The main objective of this study was to evaluate the effect of pore water velocity on hAdV transport in water saturated laboratory-scale columns packed with glass beads. The effects of pore water velocity on virus transport and retention in porous media was examined at three pore water velocities (0.39, 0.75, and 1.22 cm/min). The results indicated that all estimated average mass recovery values for hAdV were lower than those of coliphages, which were previously reported in the literature by others for experiments conducted under similar experimental conditions. However, no obvious relationship between hAdV mass recovery and water velocity could be established from the experimental results. The collision efficiencies were quantified using the classical colloid filtration theory. Average collision efficiency, α, values decreased with decreasing flow rate, Q, and pore water velocity, U, but no significant effect of U on α was observed. Furthermore, the surface properties of viruses and glass beads were used to construct classical DLVO potential energy profiles. The results revealed that the experimental conditions of this study were unfavorable to deposition and that no aggregation between virus particles is expected to occur. A thorough understanding of the key processes governing virus transport is pivotal for public health protection.

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Section: Resultsmentioning

confidence: 79%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Transport of Human Adenoviruses in Water Saturated Laboratory Columns

et al. 2015

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“…Farkas et al (2015) observed that the adsorption of rotavirus viral surrogates with similar size and pI adsorbed differently to hydrophobic media. Further differences were found in the adsorption of viral surrogates with similar size, zeta potential and hydrophobicity to porous media, suggesting that the composition of viral capsid also affects viral adhesion (Pang et al, 2014; Farkas et al, 2015). Further, Samandoulgou et al (2015) found that extremes of pH and temperature can change the mechanism of norovirus association with sediment from electrostatic to predominantly hydrophobic, as loss of ordered molecular structure in the protein head results in an increase in hydrophobic attachment sites resulting in greater adsorption of norovirus.…”

Section: Fate and Behavior Of Fecally Derived Viruses In Sedimentsmentioning

confidence: 99%

Abundance and Distribution of Enteric Bacteria and Viruses in Coastal and Estuarine Sediments—a Review

et al. 2016

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The long term survival of fecal indicator organisms (FIOs) and human pathogenic microorganisms in sediments is important from a water quality, human health and ecological perspective. Typically, both bacteria and viruses strongly associate with particulate matter present in freshwater, estuarine and marine environments. This association tends to be stronger in finer textured sediments and is strongly influenced by the type and quantity of clay minerals and organic matter present. Binding to particle surfaces promotes the persistence of bacteria in the environment by offering physical and chemical protection from biotic and abiotic stresses. How bacterial and viral viability and pathogenicity is influenced by surface attachment requires further study. Typically, long-term association with surfaces including sediments induces bacteria to enter a viable-but-non-culturable (VBNC) state. Inherent methodological challenges of quantifying VBNC bacteria may lead to the frequent under-reporting of their abundance in sediments. The implications of this in a quantitative risk assessment context remain unclear. Similarly, sediments can harbor significant amounts of enteric viruses, however, the factors regulating their persistence remains poorly understood. Quantification of viruses in sediment remains problematic due to our poor ability to recover intact viral particles from sediment surfaces (typically <10%), our inability to distinguish between infective and damaged (non-infective) viral particles, aggregation of viral particles, and inhibition during qPCR. This suggests that the true viral titre in sediments may be being vastly underestimated. In turn, this is limiting our ability to understand the fate and transport of viruses in sediments. Model systems (e.g., human cell culture) are also lacking for some key viruses, preventing our ability to evaluate the infectivity of viruses recovered from sediments (e.g., norovirus). The release of particle-bound bacteria and viruses into the water column during sediment resuspension also represents a risk to water quality. In conclusion, our poor process level understanding of viral/bacterial-sediment interactions combined with methodological challenges is limiting the accurate source apportionment and quantitative microbial risk assessment for pathogenic organisms associated with sediments in aquatic environments.

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Utility of a field deployable qPCR instrument for analyzing freshwater quality

Billington

Abeysekera

Scholes

et al. 2021

Agrosystems Geosci & Env

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A significant limitation of quantitative real-time polymerase chain reaction (qPCR) technology for environmental research has been the lack of portability of the amplification and detection equipment needed to perform qPCR assays in the field. The ability to perform qPCR assays in the field would significantly improve the utility of the technology, enabling quicker risk management decisions in acute environmental emergencies, and speeding the pace of environmental research. We tested a portable qPCR instrument, Liberty16, to evaluate if this instrument would be useful for qPCR analysis in our fieldwork. Parallel testing of the Liberty16 with our existing LightCycler 480 Instrument II revealed similar repeatability and sensitivity for the detection of targets in known and blind freshwater samples by qPCR. Both instruments were able to detect all target sequences at 20 copies per reaction and up to 66% targets in a 10-fold dilution. In seven of eight blind freshwater samples, results from both instruments were consistent for detected or nondetected target. In the other sample, the target was only detected by the Liberty16. Portable instruments of this type may open up new avenues for researchers to perform qPCR analyses in the field.

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Mimicking filtration and transport of rotavirus and adenovirus in sand media using DNA-labeled, protein-coated silica nanoparticles

Cited by 48 publications

References 52 publications

Transport of Human Adenoviruses in Water Saturated Laboratory Columns

Transport of Human Adenoviruses in Water Saturated Laboratory Columns

Abundance and Distribution of Enteric Bacteria and Viruses in Coastal and Estuarine Sediments—a Review

Utility of a field deployable qPCR instrument for analyzing freshwater quality

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