Milk Components Inhibit Acanthamoeba -Induced Cytopathic Effect

Abstract

These data, in conjunction with published findings showing that amebic proteinases are responsible for the induction of Acanthamoeba CPE, led us to propose that human mucosal secretions have the potential to provide protection against Acanthamoeba-induced CPE by an additional mechanism that is independent of IgA and that involves the inhibition of cytotoxic proteinases of amebae.

Pathogenesis of Acanthamoeba Keratitis

“…We demonstrated that IgA-depleted tears inhibit the amoeba-induced CPE, but unfractionated complete tears are three times more potent in inhibiting the amoeba-induced CPE than the IgA-depleted tears 60. Additional studies using breast milk as a model of human mucosal secretions revealed that the IgA-depleted milk also inhibited the Acanthamoeba -induced CPE 61. IgA-depleted milk proteins were fractionated into four major fractions (F1 to F4) by gel filtration on Sephadex G200.…”

Pathogenesis of Acanthamoeba Keratitis

“…We demonstrated that IgA-depleted tears inhibit the amoeba-induced CPE, but unfractionated complete tears are three times more potent in inhibiting the amoeba-induced CPE than the IgA-depleted tears 60. Additional studies using breast milk as a model of human mucosal secretions revealed that the IgA-depleted milk also inhibited the Acanthamoeba -induced CPE 61. IgA-depleted milk proteins were fractionated into four major fractions (F1 to F4) by gel filtration on Sephadex G200.…”

Camel Milk Modulates the Expression of Aryl Hydrocarbon Receptor-Regulated Genes,Cyp1a1, Nqo1, andGsta1, in Murine hepatoma Hepa 1c1c7 Cells

“…Camel samples were transported to the laboratory as soon as practical (<4 h) and were frozen at −80°C. Aqueous portion (fat-free, skimmed milk) was removed from the lipids (cream) as described before [12]. Briefly, aliquots of pooled milk samples were centrifuged at 1400 ×g for 30 minutes at 4°C, thereafter, the creamy layer consisting largely of fat was removed by filtration through a glass wool plug in a Pasteur pipette.…”

Human Milk in Dry Eye Disease: A Narrative Review with Historical Translation from the Ebers Papyrus

“…48,49 One study was excluded because it used only a rabbit corneal epithelial cellular model to test the effects of milk fractions on Acanthamoeba cytopathy. 50 Reference mining for each pertinent article was also performed, which did not reveal further pertinent studies. The nine reviewed studies were too heterogeneous in design for meta-analysis, so a narrative approach was chosen.…”

Pathogenesis of Acanthamoeba Keratitis

“…We demonstrated that IgA-depleted tears inhibit the amoeba-induced CPE, but unfractionated complete tears are three times more potent in inhibiting the amoeba-induced CPE than the IgA-depleted tears 60. Additional studies using breast milk as a model of human mucosal secretions revealed that the IgA-depleted milk also inhibited the Acanthamoeba -induced CPE 61. IgA-depleted milk proteins were fractionated into four major fractions (F1 to F4) by gel filtration on Sephadex G200.…”

Camel Milk Modulates the Expression of Aryl Hydrocarbon Receptor-Regulated Genes,Cyp1a1, Nqo1, andGsta1, in Murine hepatoma Hepa 1c1c7 Cells

“…Camel samples were transported to the laboratory as soon as practical (<4 h) and were frozen at −80°C. Aqueous portion (fat-free, skimmed milk) was removed from the lipids (cream) as described before [12]. Briefly, aliquots of pooled milk samples were centrifuged at 1400 ×g for 30 minutes at 4°C, thereafter, the creamy layer consisting largely of fat was removed by filtration through a glass wool plug in a Pasteur pipette.…”

Human Milk in Dry Eye Disease: A Narrative Review with Historical Translation from the Ebers Papyrus

“…48,49 One study was excluded because it used only a rabbit corneal epithelial cellular model to test the effects of milk fractions on Acanthamoeba cytopathy. 50 Reference mining for each pertinent article was also performed, which did not reveal further pertinent studies. The nine reviewed studies were too heterogeneous in design for meta-analysis, so a narrative approach was chosen.…”

Pathogenesis of Acanthamoeba Keratitis

“…We demonstrated that IgA-depleted tears inhibit the amoeba-induced CPE, but unfractionated complete tears are three times more potent in inhibiting the amoeba-induced CPE than the IgA-depleted tears 60. Additional studies using breast milk as a model of human mucosal secretions revealed that the IgA-depleted milk also inhibited the Acanthamoeba -induced CPE 61. IgA-depleted milk proteins were fractionated into four major fractions (F1 to F4) by gel filtration on Sephadex G200.…”

Camel Milk Modulates the Expression of Aryl Hydrocarbon Receptor-Regulated Genes,Cyp1a1, Nqo1, andGsta1, in Murine hepatoma Hepa 1c1c7 Cells

“…Camel samples were transported to the laboratory as soon as practical (<4 h) and were frozen at −80°C. Aqueous portion (fat-free, skimmed milk) was removed from the lipids (cream) as described before [12]. Briefly, aliquots of pooled milk samples were centrifuged at 1400 ×g for 30 minutes at 4°C, thereafter, the creamy layer consisting largely of fat was removed by filtration through a glass wool plug in a Pasteur pipette.…”

Human Milk in Dry Eye Disease: A Narrative Review with Historical Translation from the Ebers Papyrus

“…48,49 One study was excluded because it used only a rabbit corneal epithelial cellular model to test the effects of milk fractions on Acanthamoeba cytopathy. 50 Reference mining for each pertinent article was also performed, which did not reveal further pertinent studies. The nine reviewed studies were too heterogeneous in design for meta-analysis, so a narrative approach was chosen.…”