Migration of LHRH Neurons Derived from the Olfactory Placode in Rats.

Daikoku, Shigeo; Koide, Ikue; Chikamori-Aoyama, Mika; Shimomura, Yuichi

doi:10.1679/aohc.56.353

Cited by 31 publications

(21 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Nevertheless, although we have expected that the NAP tissue cultured in the present study does not contain components of the GnRH pulse generator other than GnRH neurons, it cannot be denied that other neurons, except for GnRH neurons, contributed to the generation of pulsatile GnRH release. In fact, we found synaptic junctions between GnRH neurons and unidentified neuronal processes in the terminal ganglion and in the septum of 16.5-day-old rat embryos [22]. Similar synaptic junctions were also seen in 7-day cultured VNO of 13.5-day-old rat embryos [Daikoku, unpubl.…”

Section: Discussionmentioning

confidence: 74%

See 1 more Smart Citation

Pulsatile Gonadotropin-Releasing Hormone (GnRH) Secretion Is an Inherent Function of GnRH Neurons, as Revealed by the Culture of Medial Olfactory Placode Obtained from Embryonic Rats

Funabashi

Daikoku²,

Shinohara³

et al. 2000

Neuroendocrinology

Self Cite

View full text Add to dashboard Cite

To determine whether gonadotropin-releasing hormone (GnRH) neurons in culture without the hypothalamus secrete GnRH in a pulsatile fashion, the nasal placode (NAP) was obtained at day 13.5 of gestation and cultured by a roller tube method. If the GnRH release occurs in a pulsatile fashion, it can be said that the pulse generator of GnRH exists inherently in each cell or community of cells in the culture. The concentration of GnRH in the NAP culture medium collected at 8-min intervals for 160 min after 2- to 4-week cultures showed that GnRH release occurred in a pulsatile fashion with a mean interpulse interval of 29.8 ± 2.3 min (n = 9). When the NAP was cultured with tissues of the forebrain vesicle (n = 3) or the hypothalamus (n = 4), GnRH was also released in a pulsatile fashion with similar intervals (27.3 ± 1.0 min for the NAP+forebrain vesicle culture and 36.0 ± 6.3 min for the NAP+hypothalamus culture) as those in cultures without brain tissues. It is concluded that pulsatile GnRH release is an inherent function of GnRH neurons.

show abstract

Section: Discussionmentioning

confidence: 74%

“…To date, primary cell cultures of GnRH neurons derived from embryonic olfactory placode have been performed in mice [21], rats [22], rhesus monkeys [23], and sheep [24]. Quite recently, it was reported that the cultured olfactory placode of rhesus monkeys releases GnRH in a pulsatile fashion [10].…”

Section: Discussionmentioning

confidence: 99%

Pulsatile Gonadotropin-Releasing Hormone (GnRH) Secretion Is an Inherent Function of GnRH Neurons, as Revealed by the Culture of Medial Olfactory Placode Obtained from Embryonic Rats

Funabashi

Daikoku²,

Shinohara³

et al. 2000

Neuroendocrinology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Recently, we found that the embryonal medial basal hypothalamus (MBH), co-cultured with the vomeronasal organ (VNO), stimulates the development and migration of LHRH neurons from the VNO. However, coculturing with the olfactory cortex, the septo-preoptic area or the cerebral cortex showed no such stimulatory activities (DAIKOKU et a!., 1993). Further, we found in vivo that most of the hypothalamic peptidergic neurons differentiate around Day 18.5 of gestation (E18.5), and that LHRH neurons establish migration to the septopreoptic area project to the median eminence on E18.5 in rats (DAIKOKU et al, 1989).…”

mentioning

confidence: 67%

“…Insulin-like growth factor I mRNA and nerve growth factor mRNA were detected in the fetal rat hypothalamus (BACH et al, 1991;OJEDA et al, 1991). In a previous study, we found that the medial basal hypothalamus co-cultured with the vomeronasal organ had stimulatory effects on the development of LHRH neurons (DAIKOKU et al, 1993). As is currently accepted, neuronal growth and survival depend on largely target-derived factors (LEIBROCK et al, 1989;DAVIES,1994;LINDEN, 1994;LINDSAY et al, 1994).…”

Section: Discussionmentioning

confidence: 91%

See 1 more Smart Citation

How the Developing Septo-Preoptic Medial Basal Hypothalamus Stimulates the Development of Placode-Derived LHRH Neurons.

Daikoku

Koide

Yoshinaka

et al. 1995

Archives of Histology and Cytology

Self Cite

View full text Add to dashboard Cite

Summary. We examined the effects of the developing cerebral cortex (CC) and septo-preoptic medial basal hypothalamus (S-MBH) on the development of LHRH neurons in vitro. The serum-free basal culture medium (BCM) was supplemented with CC or S-MBH extracts prepared from 18.5-day-old embryos or from 2-day-old newborns, and the olfactory placode (NAP) of 12-dayold embryos was cultured. The migration of LHRH neurons was found on Day 3 in the cultures supplemented with the embryonic S-MBH extract (Group 3), where the cell development proceeded showing a numerical increase of the cells and the elongation of neurites. In cultures supplemented with the newborn S-MBH extract (Group 5), the cell development was less intensive in comparison with that of Group 3, while in cultures which had no brain extracts (Group 1), the neurons failed to survive a long term culture. The effects of the CC were less than of S-MBH extracts. Analysis of the protein composition of the extracts by electrophoretic and immunoblotting examinations demonstrated a protein spot of 70-kD in the embryonic S-MBH extract. Because the protein spot was identified to be alphafetoprotein (AFP), we further examined the effects of AFP. When the anti-AFP immunoglobulin was added to the Group 3 culture, the stimulative effects of the embryonal extract were inhibited, and the addition of AFP to Group 1 cultures did not show stimulative effects. We conclude that the developing S-MBH, the migrating target of LHRH neurons, contains some essential factors for the development of LHRH neurons, but further analysis is needed to determine the chemical natures of these factors.

show abstract

Spatiotemporal appearance of developing LHRH neurons in the rat brain

Daikoku

Koide

1998

J. Comp. Neurol.

Self Cite

View full text Add to dashboard Cite

To obtain insight into the development of the heterogeneous intracerebral populations of luteinizing hormone-releasing hormone (LHRH) neurons, their spatiotemporal appearance was examined at different stages in normal rat embryos, in nasal epithelial explants in vitro, and in intrauterine nasal-operated embryos. Following the appearance of nerve cell adhesion molecule in the nasal placode at embryonic day (E) 12.5, LHRH neurons, generated in the nasal placode at E13.5, penetrated the forebrain vesicle (FV) by E14.5-15.5. After E16.5, as the FV elongated to form the olfactory bulb, the migrating neurons traversed posteriorly through the interhemispheric space to penetrate the septopreoptic (S-P) area. By E18.5, LHRH neurons were detected in the preoptic-diagonal band (P-D) area as well as in the S-P region, along with some scattered extrahypothalamic LHRH neurons. To determine the source of these neurons, we separately cultured dissected parts of E12.5 nasal pit epithelium. Neuronal generation was predominantly from the medial wall epithelium (NAP), but some LHRH neurons originated in the roof epithelium. Cocultures of the NAP (E12.5) with the FV, median eminence-arcuate complex, Rathke's pouch, mesencephalon, or medulla oblongata from E14.5 embryos revealed the ability of LHRH cells to penetrate all of these tissues. Uni- or bilateral nasal destruction was conducted at E16.5 or E15.5, respectively, and examined at E18.5 and E21.5. In the operated embryos, most LHRH neurons were present in the P-D system and some in the S-P area. This finding suggests that the neurons generated before E15.5 are primarily predisposed to form the P-D system, whereas those derived afterward form the S-P system.

show abstract

Migration of LHRH Neurons Derived from the Olfactory Placode in Rats.

Cited by 31 publications

References 33 publications

Pulsatile Gonadotropin-Releasing Hormone (GnRH) Secretion Is an Inherent Function of GnRH Neurons, as Revealed by the Culture of Medial Olfactory Placode Obtained from Embryonic Rats

Pulsatile Gonadotropin-Releasing Hormone (GnRH) Secretion Is an Inherent Function of GnRH Neurons, as Revealed by the Culture of Medial Olfactory Placode Obtained from Embryonic Rats

How the Developing Septo-Preoptic Medial Basal Hypothalamus Stimulates the Development of Placode-Derived LHRH Neurons.

Spatiotemporal appearance of developing LHRH neurons in the rat brain

Contact Info

Product

Resources

About