Migration of breast cancer cell lines in response to pulmonary laminin 332

Carpenter, Philip M.; Sivadas, Priyanka; Hua, Spencer S.; Xiao, Cally; Gutierrez, Alyssa B.; Ngo, Tuan; Gershon, Paul D.

doi:10.1002/cam4.957

Cited by 20 publications

(16 citation statements)

References 51 publications

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“…Such noncollagenous ECM molecules can activate a series of signal transduction pathways by interacting with various receptors, thereby regulating many cellular processes that include adhesion, migration, invasion, proliferation, differentiation, and morphogenesis-for which accumulating evidence indicates roles in carcinogenesis and malignancy for various cancers. [26][27][28][29][30][31][32] TMG contains multiple ECM molecules including fibronectin, laminin, fibrillin, proteoglycan, and so on. 19 Therefore, the enhanced cancer cell migration in TMG may be promoted by one or more of these ECM molecules.…”

Section: Discussionmentioning

confidence: 99%

Direct comparison of five different 3D extracellular matrix model systems for characterization of cancer cell migration

Shinsato

Doyle

et al. 2020

Cancer Reports

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Background: Three-dimensional (3D) in vitro model systems can bridge the gap between regular two-dimensional cell culture and whole-animal studies. Analyses of cancer cell migration and invasion increasingly use differing 3D systems, which may produce conflicting findings. Aims: We directly compared different 3D extracellular matrix systems for studying cancer cell migration/invasion by analyzing cell morphologies and quantifying aspects of cell migration including speed and directional persistence using automated computer-based cell tracking. Methods and results: We performed direct comparisons of five different 3D extracellular matrix cell culture systems using both HT1080 fibrosarcoma and MDA-MB-231 breast carcinoma cell lines. The reconstituted 3D systems included two types of collagen hydrogel and tissue matrix gel (TMG) vs cell-derived matrices extracted from cultured primary human or cancer-associated fibroblasts. The fibrillar matrix architecture of these systems differed. 3D rat tail collagen and TMG matrices had short, randomly oriented collagen fibrils; bovine collagen had long, larger fibril bundles; and the cellderived matrices were strongly oriented. HT1080 cells displayed rounded morphologies in all three reconstituted 3D matrices but became spindle shaped in the two cell-derived matrices. MDA-MB-231 cell morphologies were elongated in all matrices. Quantitative measures of cell migration parameters differed markedly between the different types of 3D matrix. Comparing the reconstituted matrices, cells migrated the most rapidly and furthest in TMG. Comparing TMG with cell-derived matrices, cells migrated more efficiently in the cell-derived matrices. The most notable differences were in directional persistence of migration, which was greatest in the two cell-derived matrices. Conclusion: The morphologies of matrix fibrils and cell shape, and particularly the efficiency and directionality of cell migration, differed substantially depending on the type of 3D matrix system. We suggest that it is important to employ the 3D model system that most closely resembles the matrix environment being studied for analyses of cancer cell migration and invasion.

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Section: Discussionmentioning

confidence: 99%

Direct comparison of five different 3D extracellular matrix model systems for characterization of cancer cell migration

Shinsato

Doyle

et al. 2020

Cancer Reports

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“…Generally, tumors derived from tissues normally express LM-332 might have high expression level of LM-332, such as cutaneous, esophageal, thyroid, and colon carcinomas ( 17 – 19 ). However, there is also generally decreased LM-332 expression in some tumors, such as advanced breast and prostate cancers ( 20 , 21 ).…”

Section: Discussionmentioning

confidence: 99%

Overexpression of α3, β3 and γ2 chains of laminin‑332 is associated with poor prognosis in pancreatic ductal adenocarcinoma

Zhang

Luo

et al. 2018

Oncol Lett

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Pancreatic ductal adenocarcinoma (PDA) is a worldwide health problem. Early diagnosis and assessment may enhance the quality of life and survival of patients. The present study investigated the potential correlations between the gene and protein expression of laminin-332 (LM-332 or laminin-5) and clinicopathological factors as well as evaluating its influence on the survival of patients with PDA. The expression of LM-332 subunit mRNAs in pancreatic carcinoma specimens from 37 patients was investigated by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis. Using immunohistochemical methods, the protein expressions of the three chains of LM-322 (LNα3, LNβ3 and LNγ2) were determined in 96 pancreatic carcinoma specimens, for association analysis with clinicopathological characteristics from patient data. The results of the prognosis analysis of three mRNAs expression datasets were validated in The Cancer Genome Atlas datasets. RT-qPCR results indicated that the overall relative values of LNα3 and LNγ2 mRNAs were increased in pancreatic carcinoma compared with the control. In immunostaining analyses LNα3 and LNγ2 expression was observed in all tumor tissues from the 96 patient samples. The expression levels of LNα3, LNβ3 and LNγ2 were associated with each other. LNα3 and LNγ2 positivity was significantly associated with differentiation, depth of invasion and advanced stage (P<0.05). The samples were classified into three groups: Basement membrane (B) type, cytoplasmic (C) type and mixed (M) type, according to their LNγ2 immunohistochemical expression patterns. The B type correlated significantly with differentiation (P=0.010) and the M type was significantly associated with hepatic metastasis (P=0.031). Patients with B-type LNγ2 demonstrated significantly better outcomes than patients with the C or M type (P=0.012 and P=0.003, respectively). Overexpression of the α3, β3 and γ2 chains of LM-332 may serve an important role in the progression and prognosis of PDA.

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“…Laminin-332 (LM-332) is a primary member of the laminin family. It is composed of LNα3, LNβ3 and LNγ2 chains, which are encoded by the LAMA3, LAMB3 and LAMC2 genes, respectively (8,9). The three genes each express three strands, and the subsequent formation of a heterotrimer is a key step in the formation of the LM-332 complex.…”

Section: Introductionmentioning

confidence: 99%

Laminin‑332 mediates proliferation, apoptosis, invasion, migration and epithelial‑to‑mesenchymal transition in pancreatic ductal adenocarcinoma

Huang

2020

Mol Med Rep

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The poor prognosis of patients with pancreatic ductal adenocarcinoma (PDAC) is primarily due to the invasive and metastatic behaviors of this disease. Laminin-332 (LM-332) is a key component of the basement membrane barrier, and is associated with tumor metastasis. The present study provides evidence towards the potential function of LM-332 in carcinoma, indicating the distinct roles of the three LM-332 subunits (α3, β3 and γ2) in cell proliferation, migration, invasion, apoptosis and the epithelial-to-mesenchymal transition (EMT) in cancer. The roles of the α3, β3 and γ2 subunits in the malignant biological behavior of PDAC were investigated in the present study. It was revealed that the α3, β3 and γ2 subunits were upregulated in PDAC. Inhibition of all LM-332 subunits abrogated the tumorigenic outcomes, which included cell proliferation, apoptosis, invasion, migration and EMT in vitro. However, the three LM-332 subunits had different degrees of effects on biological behavior. It was observed that LAMA3 (α3) had a stronger effect on cell proliferation, migration and invasion. In addition, LAMB3 (β3) knockdown significantly increased E-cadherin levels and decreased vimentin levels, indicating that LAMB3 was associated with EMT. Likewise, LAMC2 (γ2) mediated proliferation, apoptosis, invasion and migration. However, small interfering (si)-LAMC2 promoted the progression of EMT, which was the opposite effect to that of si-LAMB3. The LM-332 subunits (α3, β3 and γ2) may be novel therapeutic targets of PDAC in the future.

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Migration of breast cancer cell lines in response to pulmonary laminin 332

Cited by 20 publications

References 51 publications

Direct comparison of five different 3D extracellular matrix model systems for characterization of cancer cell migration

Direct comparison of five different 3D extracellular matrix model systems for characterization of cancer cell migration

Overexpression of α3, β3 and γ2 chains of laminin‑332 is associated with poor prognosis in pancreatic ductal adenocarcinoma

Laminin‑332 mediates proliferation, apoptosis, invasion, migration and epithelial‑to‑mesenchymal transition in pancreatic ductal adenocarcinoma

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