Middle age has a significant impact on gene expression during skin wound healing in male mice

Yanai, Hagai; Lumenta, David B.; Vierlinger, Klemens; Hofner, Manuela; Kitzinger, Hugo B.; Kamolz, Lars‐Peter; Nöhammer, Christa; Chilosi, Marco; Fraifeld, Vadim E.

doi:10.1007/s10522-016-9650-z

Cited by 4 publications

(3 citation statements)

References 30 publications

(36 reference statements)

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“…These data indicate that the changes of the signal network in the skin tissue under the action of electroacupuncture involve many critical signaling pathways related to skin healing. The metabolic, immune-inflammatory and growth-promoting pathways have been over-activated in the skin wound healing of the middle-aged mice, reported in previous study (36). Omega-3 polyunsaturated fatty acids (PUFAs) can promote wound healing by affecting the synthesis and activity of cytokines (37).…”

Section: Discussionmentioning

confidence: 91%

An mRNA sequencing analysis of the healing-promoting role of electroacupuncture a in rat skin wound model

Bao

et al. 2020

Ann Palliat Med

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Background: Many studies have confirmed that electroacupuncture can regulate the body's environment to treat a variety of diseases. However, there are few reports on the mechanism of electroacupuncture therapy for diseases involving skin injury. Transcriptome sequencing can reveal changes in gene expression within cells and the signaling pathways involved. In this study, we used transcriptome sequencing to study the molecular mechanisms by which electroacupuncture promotes the healing of skin lesions.Methods: A total of 10 SD rats were divided into two groups of 5: a control group and an electroacupuncture treatment group. The wound-healing area was compared between the two groups after 3 and 14 days. Then, mRNA sequencing and bioinformatics were used to analyze the changes in gene expression profiles in skin tissue after electroacupuncture stimulation.Results: (I) The wound area was significantly reduced after 3 and 14 days of electroacupuncture compared with the control group (P<0.05). (II) There was a total of 694 gene expression changes, 496 of which were upregulated and 198 of which were downregulated. Analysis of variable gene-related signaling pathways by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG), identified immuneinflammatory response, cell proliferation, tissue remodeling, cell metabolism, graft-versus-host disease, antigen processing and presentation, Th17 cell differentiation, cytokine-cytokine receptor interaction, PPAR signaling pathway, Wnt signaling pathway and other signaling pathways were changed.Conclusions: Electroacupuncture can promote wound repair, as shown by the changes in gene expression profiles during the healing of skin wounds under electroacupuncture. This study provides a scientific basis that deepens the understanding of the mechanism underlying electroacupuncture.

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“…Skin wound healing is generally divided into three stages, namely, inflammation, proliferation, and remodeling. At any of these stages of injury, age and other factors lead to obstacles and changes in skin repair [ 194 , 195 ]. In recent years, instances of hard-to-heal wounds also increases, and if not handled properly, this will affect wound healing, resulting in scars, which affect the patient's appearance.…”

Section: Application Of Microspheres In Diseasesmentioning

confidence: 99%

Progress in the application of sustained-release drug microspheres in tissue engineering

Ruan¹,

Su²,

Qin³

et al. 2022

Materials Today Bio

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“…Because of these regenerative effects, stem cells in their specific niches repair tissue after injury/disease and maintain tissue throughout life. Generally, stem cell stemness and tissue repair ability decrease with age [ 4 , 9 – 12 ]. The reduced regenerative capacity is considered as an expected response, in which wound healing takes precedence over functional recovery, resulting in scar tissue formation [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Injury-induced interleukin-1 alpha promotes Lgr5 hair follicle stem cells de novo regeneration and proliferation via regulating regenerative microenvironment in mice

Yang

Chen

et al. 2023

Inflamm Regener

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Background The hair follicles (HFs) are barely regenerated after loss in injuries in mammals as well as in human beings. Recent studies have shown that the regenerative ability of HFs is age-related; however, the relationship between this phenomenon and the stem cell niche remains unclear. This study aimed to find a key secretory protein that promotes the HFs regeneration in the regenerative microenvironment. Methods To explore why age affects HFs de novo regeneration, we established an age-dependent HFs regeneration model in leucine-rich repeat G protein-coupled receptor 5 (Lgr5) + /mTmG mice. Proteins in tissue fluids were analyzed by high-throughput sequencing. The role and mechanism of candidate proteins in HFs de novo regeneration and hair follicle stem cells (HFSCs) activation were investigated through in vivo experiments. The effects of candidate proteins on skin cell populations were investigated by cellular experiments. Results Mice under 3-week-old (3W) could regenerate HFs and Lgr5 HFSCs, which were highly correlated with the immune cells, cytokines, IL-17 signaling pathway, and IL-1α level in the regeneration microenvironment. Additionally, IL-1α injection induced de novo regeneration of HFs and Lgr5 HFSCs in 3W mouse model with a 5 mm wound, as well as promoted activation and proliferation of Lgr5 HFSCs in 7-week-old (7W) mice without wound. Dexamethasone and TEMPOL inhibited the effects of IL-1α. Moreover, IL-1α increased skin thickness and promoted the proliferation of human epidermal keratinocyte line (HaCaT) and skin-derived precursors (SKPs) in vivo and in vitro, respectively. Conclusions In conclusion, injury-induced IL-1α promotes HFs regeneration by modulating inflammatory cells and oxidative stress-induced Lgr5 HFSCs regeneration as well as promoting skin cell populations proliferation. This study uncovers the underlying molecular mechanisms enabling HFs de novo regeneration in an age-dependent model.

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Middle age has a significant impact on gene expression during skin wound healing in male mice

Cited by 4 publications

References 30 publications

An mRNA sequencing analysis of the healing-promoting role of electroacupuncture a in rat skin wound model

Progress in the application of sustained-release drug microspheres in tissue engineering

Injury-induced interleukin-1 alpha promotes Lgr5 hair follicle stem cells de novo regeneration and proliferation via regulating regenerative microenvironment in mice

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