Microwave-assisted three-liquid-phase salting-out extraction of docosahexaenoic acid (DHA)-rich oil from cultivation broths of Schizochytrium limacinium SR21

Zeb, Liaqat; Wang, Xiaojia; Zheng, Wei‐Long; Teng, Xin‐Nan; Shafiq, Muhammad; Mu, Ying; Chi, Zhanyou; Xiu, Zhilong

doi:10.1016/j.fbp.2019.09.008

Cited by 15 publications

(17 citation statements)

References 51 publications

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“…Simultaneously, 99.8% of cells and 63.3% of proteins were removed from the fermentation broth. This property that SOE could remove all of cells and most of proteins from the fermentation broth has also been proven in our previous studies [17, 28, 31, 38]. The above results demonstrated an efficient method for the recovery of CA from untreated fermentation broth, which integrated the solid–liquid separation and primary purification processes into a single unit operation, thus reducing the operation steps and cost.…”

Section: Resultssupporting

confidence: 78%

“…Because the phase compositions have significant effects on partition behavior of target molecules in SOE [17–30], the influences of phosphate and ethanol concentrations on the partitioning behaviors of CA were investigated. Moreover, the effects of pH, by changing the proportion of K 2 HPO 4 and KH 2 PO 4 , on the partition and degradation behaviors of CA were also investigated.…”

Section: Resultsmentioning

confidence: 99%

“…However, the polymer has relatively high viscosity and cost, which leads to this ATPS being rarely used on an industrial scale. As an alternative, hydrophilic organic solvent (e.g., a short‐chain alcohol)/salt based SOES, which has the advantages such as low solvent viscosity and cost, easy solvent recovery, fast phase separation rate and easy scale up, has been proven as an efficient method for primary purification of low concentration and high hydrophilicity molecules from complex substrates [17–31]. Therefore, this SOES approach is a worth trying to separate the CA from S. clavuligerus fermentation broth because of the similar features of these complex substrates.…”

Section: Introductionmentioning

confidence: 99%

“…On the other hand, process integration, which emphasizes the combination of two or more unit operations by one single‐unit operation to reduce operating steps and costs [32], is also an alternative. Interestingly enough, this SOES technology has been efficiently used to integrate the solid–liquid separation and primary purification of some target biomolecules from complex fermentation broth into a single unit operation [17, 28, 31].…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous solid–liquid separation and primary purification of clavulanic acid from fermentation broth of Streptomyces clavuligerus using salting‐out extraction system

Wang

Qin

et al. 2021

Engineering in Life Sciences

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Clavulanic acid (CA) is usually used together with other β‐lactam antibiotics as combination drugs to inhibit bacterial β‐lactamases, which is mainly produced from the fermentation of microorganism such as Streptomyces clavuligerus. Recently, it is still a challenge for downstream processing of low concentration and unstable CA from fermentation broth with high solid content, high viscosity, and small cell size. In this study, an integrated process was developed for simultaneous solid–liquid separation and primary purification of CA from real fermentation broth of S. clavuligerus using salting‐out extraction system (SOES). First, different SOESs were investigated, and a suitable SOES composed of ethanol/phosphate was chosen and further optimized using the pretreated fermentation broth. Then, the optimal system composed of 20% ethanol/15% K2HPO4 and 10% KH2PO4 w/w was used to direct separation of CA from untreated fermentation broth. The result showed that the partition coefficient (K) and recovery yield (Y) of CA from untreated fermentation broth were 29.13 and 96.8%, respectively. Simultaneously, the removal rates of the cells and proteins were 99.8% and 63.3%, respectively. Compared with the traditional method of membrane filtration or liquid–liquid extraction system, this developed SOES showed the advantages of simple operation, shorter operation time, lower process cost and higher recovery yield of CA. These results demonstrated that the developed SOES could be used as an attractive alternative for the downstream processing of CA from real fermentation broth.

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Section: Resultssupporting

confidence: 78%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Simultaneous solid–liquid separation and primary purification of clavulanic acid from fermentation broth of Streptomyces clavuligerus using salting‐out extraction system

Wang

Qin

et al. 2021

Engineering in Life Sciences

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“…The efficient incorporation of KO into the cell membrane makes it a better choice than fish oil [12]. The EPA and DHA are omega‐3 polyunsaturated fatty acids which are the important component of the human cerebral cortex, skin, and brain [13–15]. Moreover, EPA and DHA have been documented for nutritional and health‐related applications [16].…”

Section: Introductionmentioning

confidence: 99%

Three‐liquid‐phase salting‐out extraction of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)‐rich oils from Euphausia superba

Zeb

Teng

Shafiq

et al. 2021

Engineering in Life Sciences

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The TLPSOES parameters were optimized by response surface methodology using Box–Behnken design, which were 16.5% w/w of ammonium citrate, 17.5% w/w of ethanol, and 46% w/w of n‐hexane at 70 min of stirring time. Under optimized conditions the extraction efficiency attained was 90.91 ± 0.97% of EPA, 90.02 ± 1.04% of DHA, and 91.85 ± 1.11% of KO in the top n‐hexane phase. The highest extraction efficiency of proteins and flavonoids, i.e. 88.34 ± 1.35% and 79.67 ± 1.13%, was recorded in the solid interface and ethanol phase, respectively. The KO extracted by TLPSOES system consisted of lowest fluoride level compared to the conventional method and whole wet krill biomass. The TLPSOES is a potential candidate for nutraceutical industry of KO extraction from wet krill biomass.

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Simultaneous extraction of DHA‐ and EPA‐rich oil and separation of proteins from Antarctic krill using three‐phase partitioning system of cosolvents and organic salt

Zeb

Teng

Shafiq

et al. 2022

J of Chemical Tech & Biotech

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BACKGROUND The nutritional and functional potential of eicosapentaenoic acid (EPA)‐ and docosahexaenoic acid (DHA)‐rich oil from Antarctic krill has continued to attract attention worldwide. Therefore, a highly effective approach is essential for extracting Antarctic krill's DHA/EPA‐rich oil and proteins from wet biomass in the same system. RESULTS A cosolvent three‐phase partitioning system (TPPS) was developed to simultaneously extract DHA/EPA‐rich oil and separate proteins (PT) from wet Antarctic krill (Euphausia superba) biomass. The COSMO‐RS was used to screen cosolvents for the extraction of EPA and DHA. The TPPS parameters were optimized by response surface methodology using a central‐composite design, which consisted of 52% (w/w) of cosolvents (t‐butanol:n‐hexane (4:6)), 20% (w/w) of ammonium citrate, 500 rpm of stirring speed, 37 °C of temperature, and 60 min of extraction time. Under the optimized conditions, the extraction efficiency of EPA and DHA was 95.36 ± 0.94% and 93.85 ± 1.31% in the top cosolvents phase, respectively, as well as 86.57 ± 0.98% of proteins in the middle interface. Compared to supercritical CO2 (SCO2) and three‐liquid‐phase salting‐out extraction system (TLPSOES), the recovery rate of krill oil (KO) extracted by TPPS (96.48 ± 1.14%) was much higher than that of the SCO2 (67.8%) and of the TLPSOES method (91%). CONCLUSION This study demonstrates that cosolvent TPPSs can extract krill oil from wet krill biomass with higher extraction efficiency, and that the obtained krill oil showed potential antioxidant effects compared to commercial fish oil. Therefore, the cosolvent TPPS could be an alternative in the nutraceutical industry. © 2022 Society of Chemical Industry (SCI).

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Microwave-assisted three-liquid-phase salting-out extraction of docosahexaenoic acid (DHA)-rich oil from cultivation broths of Schizochytrium limacinium SR21

Cited by 15 publications

References 51 publications

Simultaneous solid–liquid separation and primary purification of clavulanic acid from fermentation broth of Streptomyces clavuligerus using salting‐out extraction system

Simultaneous solid–liquid separation and primary purification of clavulanic acid from fermentation broth of Streptomyces clavuligerus using salting‐out extraction system

Three‐liquid‐phase salting‐out extraction of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)‐rich oils from Euphausia superba

Simultaneous extraction of DHA‐ and EPA‐rich oil and separation of proteins from Antarctic krill using three‐phase partitioning system of cosolvents and organic salt

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