Microstructure and antibacterial properties of AISI 420 stainless steel implanted by copper ions

Zhou, Dan; Ni, Hongwei; Xu, Bin; Xiong, Juan; Ping-yuan, Xiong

doi:10.1016/j.tsf.2005.06.100

Cited by 126 publications

(57 citation statements)

References 8 publications

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“…Copper ions are absorbed onto the bacterial cell surface, imparting damage to the cell membrane by solidifying protein structure or altering enzyme function (Ohsumi et al 1988;Dan et al 2005). Bacterial cells are immobilized and become inactivated by the presence of copper nanoparticles in the growth medium, which results in hampering of their replication process, with subsequent cell death (Hu and Xia 2006).…”

Section: Resultsmentioning

confidence: 99%

Investigations into the antibacterial behavior of copper nanoparticles against Escherichia coli

et al. 2010

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Zerovalent copper nanoparticles (Cu 0 ) of 12 nm size were synthesized using an inert gas condensation method in which bulk copper metal was evaporated into an inert environment of argon with subsequent cooling for nucleation and growth of nanoparticles. Crystalline structure, morphology and estimation of size of nanoparticles were carried out by X-ray diffraction and transmission electron microscopy. The antibacterial activity of these nanoparticles against the Gram-negative bacterium Escherichia coli was assessed in liquid as well as solid growth media. It was observed from scanning electron microscopic analysis that the interaction of copper nanoparticles with E. coli resulted in the formation of cavities/pits in the bacterial cell wall. The antibacterial property of copper nanoparticles was attributed mainly to adhesion with bacteria because of their opposite electrical charges, resulting in a reduction reaction at the bacterial cell wall. Nanoparticles with a larger surface-to-volume ratio provide more efficient means for antibacterial activity.

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Section: Resultsmentioning

confidence: 99%

Investigations into the antibacterial behavior of copper nanoparticles against Escherichia coli

et al. 2010

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“…These ions damage the cell membrane and penetrate into the bacteria. Cu-ions that have the strong reduction can extract the electrons from the bacteria, causing their cytoplasm to run o and oxidizing their cell nucleus and killing them [34,35].…”

Section: Antibacterial Assessmentmentioning

confidence: 99%

Effect of Cu2+ ion on Biological Performance of Nanostructured Fluorapatite Doped with Copper

2017

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Abstract. Nanostructured copper-doped uorapatite (Cu x .Ca (10 x) .(PO 4 ) 6 .F 2 ) having crystallite sizes of 19, 29, and 34 nm at x = 0:9, 0.4, and 0.0, respectively, was synthesized by planetary ball milling of CaO, P2O5, CaF2, and CuO powders. Speci cations of the products were determined by Fourier-transform infrared spectroscopy, eld emission scanning electron microscopy, transmission electron microscopy, and X-ray di raction analyses. In-vitro studies and Mossman's Tetrazole Test (MTT) assays were also conducted by incubating Cu x .Ca (10 x) .(PO 4 ) 6 .F 2 powder into Kokubo's Simulated Body Fluid (SBF) and against BT-20 cell, respectively, to determine bioactivity and biocompatibility of the materials. Antibacterial e ects on Staphylococcus aureus were assessed by the disc di usion test method. Measurements showed that the rate of formation of uorapatite was lowered by Cu content. Besides, in-vitro experiments showed the same SBF interacted apatite precipitation for all samples. In contrast, MTT assays revealed di erent behavior for pure uorapatite and apatite with x = 0:9 Cu against BT-20 cell after 24 h of incubation. This highlights the increase of uorapatite cytotoxicity when Cu ion is present in the apatite structure. Copper-doped uorapatite was, however, desirably antibacterial. This stemmed from copper ions interactions with the bacterial metabolism which resulted in enzymes neutralization and copper-doped uorapatite antibacterial behavior.

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“…When excess copper binds to these enzymes, their activity grinds to a halt. [6,7]. Figure 4 shows the activity of copper on various microbes.…”

Section: Mechanism Of Copper's Antimicrobial Activitymentioning

confidence: 99%

Copper Thin Film Sputtered on Aisi 316l for Antimicrobial Property

Arulkumar¹,

Krishnan²,

Kedharnath³

et al. 2013

IJSEA

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Abstract:The surgical and other biological instruments are made of Stainless Steel AISI 316L which lacks antimicrobial properties. Copper is coated on Stainless Steel substrate using DC Magnetron sputtering which is used to achieve required film of thickness (0.5-8µm). The deposition pressure, substrate temperature, power supply, distance between the specimen and target are optimized and maintained constant, while the sputtering time (30-110 minutes) is varied. The sputtered copper thin film's morphology, compositional is characterized by SEM and EDAX. X-ray diffraction analysis shows copper oriented on (111) and (002) and copper oxide on (111) planes. The contact angle of copper thin film is 92° while AISI316L shows 73°. The antimicrobial studies carried in Staphylococcus aureus, Escherichia Coli, Klebsiella pneumonia and Candida albicans shows that CFU/mL was reduced to 30 after 24 hours. The cell viability is studied by MTT assay test on Vero cell line for 24 hours, 48 hours and 72 hours and average cell viability is 43.85%. The copper release from the thin film to the culture medium is 4826µg/L (maximum) is estimated from AAS studies. The bacteria and fungi are found to be destroyed by the copper thin film but do not show much reaction with living Vero cells.

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Microstructure and antibacterial properties of AISI 420 stainless steel implanted by copper ions

Cited by 126 publications

References 8 publications

Investigations into the antibacterial behavior of copper nanoparticles against Escherichia coli

Investigations into the antibacterial behavior of copper nanoparticles against Escherichia coli

Effect of Cu2+ ion on Biological Performance of Nanostructured Fluorapatite Doped with Copper

Copper Thin Film Sputtered on Aisi 316l for Antimicrobial Property

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