Microscopic investigations of <i>Ocotea paranaensis</i>―A Brazilian endemic and endangered species

Gribner, Caroline; Barbosa, Vanessa; Monteiro, Luciane Mendes; Swiech, Juliane Nadal Dias; Raman, Vijayasankar; Budel, Jane Manfron; Miguel, Obdúlio Gomes; Montrucchio, Deise Prehs; Dias, Josiane de Fátima Gaspari

doi:10.1002/jemt.24186

Cited by 3 publications

(5 citation statements)

References 35 publications

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“…It consists of a mass of small (2-5 μm) individual crystals within a single cell, assuming different forms (Upton et al, 2011). Most studies have described these structures simply as crystal sands without detailing the micromorphology of the crystals (Konyar et al, 2014;Santos et al, 2015;Capacio & Belonias, 2018;Gribner et al, 2022). In the present study, nine morphotypes of crystal sands are recorded in the perimedullary region of stems of Baccharisspecies: arrow-shaped (sar) (Fig.…”

Section: Morphology and Distribution Of Crystalsmentioning

confidence: 63%

“…However, other compositions can also occur, such as calcium carbonate (CaCO 3 ) crystals, or even silica crystals (SiO 2 ) (Bosqueiro, 1995;Webb, 1999). Crystals in plants have been reported as calcium oxalate using EDS in many studies (Lersten & Horner, 2011;Saulle et al, 2018;Santos et al, 2018;Almeida et al, 2020;Klider et al, 2020;Brito et al, 2021;D'Almeida et al, 2021;Pauzer et al, 2021;Gribner et al, 2022). Only a few studies have reported calcium sulfate crystals (Storey & Thomson, 1994;Pritchard et al, 2000;He et al, 2012).…”

Section: Morphology and Distribution Of Crystalsmentioning

confidence: 99%

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Calcium oxalate crystal macropattern and its usefulness in the taxonomy of Baccharis (Asteraceae)

Raeski

Heiden

Novatski

et al. 2023

Preprint

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This study provides a comprehensive account of the various types of calcium oxalate crystals found in the genus Baccharis and assesses the exceptional value of crystal macropatterns for the taxonomy of the genus. The morphotype, occurrence and chemical composition of the crystals found in the stems and leaves are studied. The 44 species included in this study were selected based on a broad phylogeny-based sampling covering seven subgenera and 31 sections. These species were chosen to represent all the main phylogenetic lineages of Baccharis; thus, the sampling also represents a comprehensive coverage concerning evolutionary significance for such a large and environmentally and economically important plant group. The samples were analyzed by light microscopy, scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDS). Several morphotypes of crystals, including druses, crystal sand, styloids and prisms, were present. Based on their chemical composition, the crystals were classified as pure calcium oxalate, mixtures of oxalates and sulfates, and mixtures of oxalates, sulfates and silica. The crystal macropatterns observed in this study aid in the species identification and provide novel data for the taxonomy of Baccharis.

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Section: Morphology and Distribution Of Crystalsmentioning

confidence: 63%

Section: Morphology and Distribution Of Crystalsmentioning

confidence: 99%

Calcium oxalate crystal macropattern and its usefulness in the taxonomy of Baccharis (Asteraceae)

Raeski

Heiden

Novatski

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…It consists of a mass of small (2–5 μm) individual crystals within a single cell, assuming different forms (Upton et al, 2011). Most studies have described these structures simply as crystal sands without detailing the micromorphology of the crystals (Capacio & Belonias, 2018; Gribner et al, 2022; Konyar & Dane, 2014; Santos et al, 2015). In the present study, nine morphotypes of crystal sands are recorded in the perimedullary region of stems of Baccharis species: arrow‐shaped (sar) (Figure 2a) in B. pauciflosculosa , B. platypoda , B. reticularioides and B. thymifolia ; bipyramidal (sbp) (Figure 2e–g) in B. paniculata B. potrerillana , and B. pluricapitulata ; cuneiform (scn) (Figure 2h) in B. thymifolia ; rounded (sro) (2i, j) in B .…”

Section: Resultsmentioning

confidence: 99%

“…However, other compositions can also occur, such as calcium carbonate (CaCO 3 ) crystals, or even silica crystals (SiO 2 ) (Bosqueiro, 1995; Webb, 1999). Crystals in plants have been reported as calcium oxalate using EDS in many studies (Lersten & Horner, 2011; Saulle et al, 2018; Santos et al, 2018; Almeida et al, 2020; Klider et al, 2020; Brito et al, 2021; D'Almeida et al, 2021; Pauzer et al, 2021; Gribner et al, 2022). Only a few studies have reported calcium sulfate crystals (He et al, 2012; Pritchard et al, 2000; Storey & Thomson, 1994).…”

Section: Resultsmentioning

confidence: 99%

Calcium oxalate crystal macropattern and its usefulness in the taxonomy of Baccharis (Asteraceae)

Raeski

Heiden

Novatski

et al. 2023

Microscopy Res & Technique

Self Cite

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This study provides a comprehensive account of the various types of calcium oxalate crystals found in the genus Baccharis and assesses the exceptional value of crystal macropatterns for the taxonomy of the genus. The morphotype, occurrence, and chemical composition of the crystals found in the stems and leaves are studied. The 44 species included in this study were selected based on a broad phylogeny‐based sampling covering seven subgenera and 31 sections. These species were chosen to represent all the main phylogenetic lineages of Baccharis; thus, the sampling also represents a comprehensive coverage concerning evolutionary significance for such a large and environmentally and economically important plant group. The samples were analyzed by light microscopy, scanning electron microscopy (SEM), and energy‐dispersive x‐ray spectroscopy (EDS). Several morphotypes of crystals, including druses, crystal sand, styloids and prisms, were present. Based on their chemical composition, the crystals were classified as pure calcium oxalate, mixtures of oxalates and sulfates, and mixtures of oxalates, sulfates, and silica. The crystal macropatterns observed in this study aid in species identification and provide novel data for the taxonomy of Baccharis. Research Highlights Most species of Baccharis have a specific crystalline pattern. Each species produces a crystal morphotype or a set of morphotypes specific to it. The crystals observed are formed by calcium oxalate.

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“…The SAQ and SAT samples were used to prepare TSs. Color, taste, texture, and TS of stems were investigated (Gribner et al, 2022).…”

Section: Methodsmentioning

confidence: 99%

Pharmacognostic standardization and machine learning–based investigations on Akebia quinata and Akebia trifoliata

Qiu

Shi

2023

Biomedical Chromatography

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Currently, Akebiae Caulis is being used in clinical practice, but there are few reseaches on its different varieties. To ensure the accuracy and effectiveness of clinical practice, this study distinguished the Akebia quinata (Thunb.) Decne. and Akebia trifoliata (Thunb.) Koidz, using organoleptic evaluation, microscopic observation, fluorescence reaction, physicochemical properties, thin‐layer chromatography, IR spectroscopy, HPLC, four machine learning models, and in vitro antioxidant methods. Analysis of the powders of these two varieties using optical microscopy revealed the presence of starch granules, cork cells, crystal fibers, scalariform vessels, and wood fibers. Scanning electron microscopy revealed the presence of scalariform vessels, pitted vessels, wood fibers, and calcium oxalate crystals. Several tissues, including the cork layer, fiber population, cortex, phloem, pith, xylem, and ray, were found in the transverse section. In addition, thin‐layer chromatography was used to identify two components: oleanolic acid and calceolarioside B; 11 common peaks were identified in 15 batches of SAQ and 5 batches of SAT by using HPLC. Support vector machine, BP neural networks, and GA‐bp neural networks were able to predict 100% accurately of the different origins of stem of Akebia quinate (Thunb.) Decne (SAQ) and Akebia trifoliata (Thunb.) Koidz (SAT). Extreme learning machine achieved a correct rate of 87.5%. Meanwhile, Fourier‐transform infrared spectroscopy fingerprint identified nine characteristic absorption peaks of the secondary metabolites of SAQ and SAT. 2,2‐Diphenyl‐1‐1‐picrylhydrazyl experiment revealed that the IC50 values of SAQ and SAT extracts were 155.49 and 128.75 μg/ml, respectively. For the 2,2′‐azino‐bis‐(3‐ethylbenzothiazoline‐6‐sulfonic acid) assay, the IC50 value of SAT extract was found to be 269.24 μg/ml, which was lower than that of SAQ extract (IC50 = 358.99 μg/ml). This study successfully used different methods to differentiate between A. quinata (Thunb.) Decne. and A. trifoliata (Thunb.) Koidz., to help decide on which type to use for clinical application.

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Microscopic investigations of Ocotea paranaensis―A Brazilian endemic and endangered species

Cited by 3 publications

References 35 publications

Calcium oxalate crystal macropattern and its usefulness in the taxonomy of Baccharis (Asteraceae)

Calcium oxalate crystal macropattern and its usefulness in the taxonomy of Baccharis (Asteraceae)

Calcium oxalate crystal macropattern and its usefulness in the taxonomy of Baccharis (Asteraceae)

Pharmacognostic standardization and machine learning–based investigations on Akebia quinata and Akebia trifoliata

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