Microscope imaging mass spectrometry with a reflectron

Abstract

Multiple-ion-ejection multi-reflection time-of-flight mass spectrometry for singlereference mass measurements with lapping ion species Review of Scientific Instruments 91, 023201 (2020);

High-Resolution Ion Microscope Imaging over Wide Mass Ranges Using Electrodynamic Postextraction Differential Acceleration

“…Figure a demonstrates that this property holds for electrodynamic PEDA as well, as the average recorded spatial resolution is 25 ± 3 μm between 300 and 600 Da. These results are consistent with the simulation (red trace) and with the expected resolution of the ion microscope with and without a PEDA pulse applied. ,, …”

“…These results are consistent with the simulation (red trace) and with the expected resolution of the ion microscope with and without a PEDA pulse applied. 21 , 28 , 33 …”

“…The instrument has an adaptable chamber that can be configured as an approximately linear ion microscope or as a two-stage system where the temporal PEDA focus is used as a pseudosource for a reflectron. 33 The linear configuration was used here to better compare the data with previous experiments, which shared the same ion optical system but a shorter drift distance. 28 The ion optical dimensions are therefore the same as those used for Figures 1 b and 2 but with L D ≃ 240 cm.…”

“…Electrodynamic PEDA was tested using a time-of-flight microscope imaging mass spectrometer. The instrument has an adaptable chamber that can be configured as an approximately linear ion microscope or as a two-stage system where the temporal PEDA focus is used as a pseudosource for a reflectron . The linear configuration was used here to better compare the data with previous experiments, which shared the same ion optical system but a shorter drift distance .…”

“…These results are consistent with the simulation (red trace) and with the expected resolution of the ion microscope with and without a PEDA pulse applied. 21,28,33 The spatial resolution was determined independently for each recorded ion by gating the CCD camera over the m/z range of interest to produce images of the gridded dye samples, such as the one shown in Figure 5b. The spatial resolutions were then determined by averaging the 20−80% intensity rises (Figure 5c) of the grid patterns to determine the corresponding Gaussian standard deviations σ 36 and then dividing them by the image magnification M according to 37…”