Microscale Assay Monitors Algal Growth Characteristics

Bernd, Karen K.; Cook, Nathaniel

doi:10.2144/02326bm08

Cited by 6 publications

(3 citation statements)

References 2 publications

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“…To quantify C. reinhardii , we generated a reference curve using a Thoma cell counting Chamber and measured the corresponding optical density with a plate reader (Tecan infinite 200 with the software MagellanPro v7.3 2019) for OD 750 nm [21] with pure Mix-Medium as a blank, covering a C. reinhardii concentration range from 0 to 22 [10 6 cells mL -1 ] (SI Fig. S1).…”

Section: Methodsmentioning

confidence: 99%

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Herbivory can increase plant fitness via reduced interspecific competition – evidence from models and mesocosms

Böttner,

Dudenhausen,

Nouere

et al. 2024

Preprint

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Herbivores are generally considered to reduce plant fitness. However, as in natural communities herbivores often feed on several competing plant species, herbivores can also increase plant fitness by reducing interspecific competition among plants. In this study, we developed a testable model to predict plant fitness in the presence of an interspecific competitor and an herbivore that feeds on both plant species. Our model allows to predict at which herbivore and competitor densities the focal species will benefit from herbivory. This can be estimated by quantifying the effects of the herbivore on the fitness of the focal plant and on its competitor, and by estimating the levels of intra- and interspecific competition in a pair-wise fashion, respectively. We subsequently validated the model in indoor microcosms using three interacting species: an aquatic macrophyte (the giant duckweedSpirodela polyrhiza), its native competitors (green algae), and its native herbivore (the pond snailLymnaea stagnalis). Additional outdoor mesocosm experiments supported our model under natural conditions. Together, this study provides a conceptual framework to understand how herbivores shape plant fitness in a community context.

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Section: Methodsmentioning

confidence: 99%

“…As the abundance of S. polyrhiza , its herbivore L. stagnalis , and its competitor Chlamydomonas spp. can be quantified and manipulated across multiple generations [21], these interacting species are ideal for testing the direct and indirect effects of herbivores on plant fitness.…”

Section: Introductionmentioning

confidence: 99%

Herbivory can increase plant fitness via reduced interspecific competition – evidence from models and mesocosms

Böttner,

Dudenhausen,

Nouere

et al. 2024

Preprint

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“…The concentration of viable cells following alkalization was estimated with the Serial Dilution Culture -Most Probable Number (SDC -MPN) assay (McCrady, 1915;Throndsen, 1978), using the methodology developed and validated by MacIntyre et al (2018MacIntyre et al ( , 2019. The method was modified to use sterile 24-well polystyrene cell culture plates (Sigma Aldrich), based on the approach described by Bernd and Cook (2002), rather than individual tubes. The assays were set up with 8 replicates in each of 6 tiers of successive 10 -1 dilutions.…”

Section: Modified Serial Dilution Culture -Most Probable Number Assaymentioning

confidence: 99%

Assessing the impacts of simulated Ocean Alkalinity Enhancement on viability and growth of near-shore species of phytoplankton

Oberlander,

Burke,

London

et al. 2024

Preprint

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Abstract. Over the past 250 years, atmospheric CO2 concentrations have risen steadily from 277 ppm to 405 ppm, driving global climate change. In response, new technologies are being developed to reduce emissions and to remove carbon from the atmosphere using negative emission technologies (NETs). One proposed NET is Ocean Alkalinity Enhancement (OAE), which would mimic the ocean’s natural weathering processes, raising alkalinity and pH and sequestering carbon dioxide from the atmosphere. The potential impacts of OAE were assessed through an analysis of prior studies investigating the effects of elevated pH on phytoplankton growth rates and by experimental assessment of the pH-dependence of viability and growth rates in two near-shore isolates of phytoplankton. Viability was assessed with a modified Serial Dilution Culture – Most Probable Number assay. Chlorophyll a fluorescence was used to test for changes in photosynthetic competence and apparent growth rates. There were no significant impacts on the viability or growth rates of the diatom Thalassiosira pseudonana and the prymnesiophyte Diacronema lutheri (formerly Pavlova lutheri) with short-term (10-minute) exposure to elevated pH. However, there was a significant decrease in growth rates with long-term (days) exposure to elevated pH. Short-term exposure is anticipated to more closely mirror the natural systems in which OAE will be implemented because of system flushing and dilution. These preliminary findings suggest that there will be little to no impact on a variety of taxonomic groups of phytoplankton when OAE occurs in naturally flushed systems.

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References to Volume 1

2009

The Chlamydomonas Sourcebook

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Microscale Assay Monitors Algal Growth Characteristics

Cited by 6 publications

References 2 publications

Herbivory can increase plant fitness via reduced interspecific competition – evidence from models and mesocosms

Herbivory can increase plant fitness via reduced interspecific competition – evidence from models and mesocosms

Assessing the impacts of simulated Ocean Alkalinity Enhancement on viability and growth of near-shore species of phytoplankton

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