Microsatellite primers in <i>Oenothera harringtonii</i> (Onagraceae), an annual endemic to the shortgrass prairie of Colorado

Skogen, Krissa A.; Hilpman, Evan T.; Todd, Sadie L.; Fant, Jeremie B.

doi:10.3732/ajb.1200003

Cited by 7 publications

(6 citation statements)

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“…After repeating these simulations with different combinations of the microsatellite markers described in Skogen et al . (), a set of seven markers was identified that maximized the resolving power of simulated paternity tests. Offspring were therefore assayed at these loci, and the parental data set (Rhodes et al .…”

Section: Methodsmentioning

confidence: 99%

“…Simulations were based on observed allele frequencies in the parental data set (Rhodes et al 2014) and were parameterized as follows: 10 000 simulated offspring, 350 candidate fathers, 95% of candidate fathers sampled, genotyping error rate of 1% and a minimum of four typed loci. After repeating these simulations with different combinations of the microsatellite markers described in Skogen et al (2012), a set of seven markers was identified that maximized the resolving power of simulated paternity tests. Offspring were therefore assayed at these loci, and the parental data set (Rhodes et al 2014) was truncated to match.…”

Section: Dna Extraction and Genotypingmentioning

confidence: 99%

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Pollinator identity and spatial isolation influence multiple paternity in an annual plant

Rhodes

Fant²,

Skogen³

2017

Molecular Ecology

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The occurrence and extent of multiple paternity is an important component of variation in plant mating dynamics. However, links between pollinator activity and multiple paternity are generally lacking, especially for plant species that attract functionally diverse floral visitors. In this study, we separated the influence of two functionally distinct floral visitors (hawkmoths and solitary bees) and characterized their impacts on multiple paternity in a self-incompatible, annual forb, Oenothera harringtonii (Onagraceae). We also situated pollinator-mediated effects in a spatial context by linking variation in multiple paternity to variation in plant spatial isolation. We documented pronounced differences in the number of paternal sires as function of pollinator identity: on average, the primary pollinator (hawkmoths) facilitated mating with nearly twice as many pollen donors relative to the secondary pollinator (solitary bees). This effect was consistent for both isolated and nonisolated individuals, but spatial isolation imposed pronounced reductions on multiple paternity regardless of pollinator identity. Considering that pollinator abundance and pollen dispersal distance did not vary significantly with pollinator identity, we attribute variation in realized mating dynamics primarily to differences in pollinator morphology and behaviour as opposed to pollinator abundance or mating incompatibility arising from underlying spatial genetic structure. Our findings demonstrate that functionally distinct pollinators can have strongly divergent effects on polyandry in plants and further suggest that both pollinator identity and spatial heterogeneity have important roles in plant mating dynamics.

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Section: Methodsmentioning

confidence: 99%

Section: Dna Extraction and Genotypingmentioning

confidence: 99%

Pollinator identity and spatial isolation influence multiple paternity in an annual plant

Rhodes

Fant²,

Skogen³

2017

Molecular Ecology

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“…DNA was quantified using a Nanodrop (Nano-Drop Technologies, Wilmington, Delaware, U.S.A.) and diluted to a final concentration of 50-100 mg/ml. All individuals were genotyped using 12 nuclear microsatellite markers (Skogen et al, 2012;Rhodes et al, 2014) and four plastid microsatellite makers (Lewis et al, 2016). The nuclear microsatellite markers were fluorescently labeled (WellRed D2, D3, or D4, Sigma-Proligo, St. Louis, Missouri, U.S.A.) while the plastid samples were labeled using a two-step polymerase chain reaction (PCR) (Skogen et al, 2012;Lewis et al, 2016).…”

Section: Molecular Datamentioning

confidence: 99%

Hawkmoth Pollination Facilitates Long-distance Pollen Dispersal and Reduces Isolation Across a Gradient of Land-use Change

Skogen

Overson

Hilpman

et al. 2019

annals

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Land-use change is among the top drivers of global biodiversity loss, which impacts the arrangement and distribution of suitable habitat for species. Population-level effects include increased isolation, decreased population size, and changes to mutualistic and antagonistic interactions. However, the extent to which species are impacted is determined by life history characteristics including dispersal. In plants, mating dynamics can be changed in ways that can negatively impact population persistence if dispersal of pollen and/or seed is disrupted. Long-distance dispersal has the potential to buffer species from the negative impacts of land-use change. Biotic vectors of long-distance dispersal have been less frequently studied, though specific taxa are known to travel great distances. Here, we describe population genetic diversity and structure in a sphingophilous species that is experiencing habitat fragmentation through land-use change, Oenothera harringtonii W. L. Wagner, Stockh. & W. M. Klein (Onagraceae). We use 12 nuclear and four plastid microsatellite markers and show that pollen dispersal by hawkmoths drives high gene flow and low population differentiation despite a range-wide gradient of land-use change and habitat fragmentation. By separating the contributions of pollen and seed dispersal to gene flow, we show that most of the genetic parameters are driven by hawkmoth-facilitated long-distance pollen dispersal, but populations with small, effective population sizes experience higher levels of relatedness and inbreeding. We discuss considerations for conservation efforts for this and other species that are pollinated by long-distance dispersers.

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“…However, given the small number of individuals used to perform the analysis (three per population), we cannot make any assumptions about the species' genetic diversity and genetic structure. When three populations were evaluated, the genetic variation found was lower than that reported for Oenothera harringtonii and Oenothera biennis, for which the same primers were developed and tested [47,48]. The number of alleles found in O. drummondii was lower for most markers tested.…”

Section: Discussionmentioning

confidence: 68%

“…We tested a combination of plastid and nuclear microsatellite loci and screened 28 published markers (nuclear: Oenbi2diA_C10, Oenbi2diA_E9, Oenbi2triA_A1, Oenbi2triA_A5, Oenbi2triA_C6, Oenbi2triA_D3, Oenbi2triA_E4, Oenbi2triA_F5, Oenbi2triA_H1, Oenbi2triA_H2, Oenbi39tri10, OenhaB105, OenhaC4, OenhaC105, OenhaC106, OenhaC126, OenhaD2, OenhaD5, OenhaD102, OenhaD111, OenhaD118, Oenbi2tri2, Oenbi39di2, Oenbi39tri4, plastid: OenelCp3, OenelCp5, OenelCp11, OenelCp12) [47][48][49]. Ten markers were designed for Oenothera harringtonii W.L.…”

Section: Primer Selectionmentioning

confidence: 99%

Transferability of Microsatellite Markers Developed in Oenothera spp. to the Invasive Species Oenothera drummondii Hook. (Onagraceae)

et al. 2020

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Oenothera drummondii Hook. (Onagraceae) has life-history traits that make it an invasive species. Native populations are distributed along the coastal dunes from North Carolina in the United States to Tabasco in the Gulf of Mexico. It has been reported as an invasive species in Spain, Israel, and China, where this species can successfully colonize and dominate if the environmental conditions are appropriate. In South Africa, Australia, New Zealand, and France, it is reported to be naturalized. In this study, 28 microsatellite markers developed for other Oenothera species were evaluated for cross-amplification in O. drummondii. Nine primers showed consistent amplification and were polymorphic. Polymorphism was assessed in three populations from both native and invaded areas. Results indicated generalized low genetic variability. Three loci showed significant deviations from the Hardy Weinberg equilibrium, associated with null alleles’ presence. The observed heterozygosity and inbreeding coefficient reflected a generalized excess of homozygotes, particularly in the invaded population “El Dique”, likely due to allele fixation. High genetic differentiation was found between the three populations. These results highlight the accuracy of these markers for future population genetic studies in O. drummondii.

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Microsatellite primers in Oenothera harringtonii (Onagraceae), an annual endemic to the shortgrass prairie of Colorado

Abstract: These results indicate the utility of these markers in O. harringtonii for future studies of genetic structure, gene flow, and parentage as well as their applicability in other members of the O. caespitosa species complex.

Cited by 7 publications

References 10 publications

Pollinator identity and spatial isolation influence multiple paternity in an annual plant

Pollinator identity and spatial isolation influence multiple paternity in an annual plant

Hawkmoth Pollination Facilitates Long-distance Pollen Dispersal and Reduces Isolation Across a Gradient of Land-use Change

Transferability of Microsatellite Markers Developed in Oenothera spp. to the Invasive Species Oenothera drummondii Hook. (Onagraceae)

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