Microsatellite markers for the fungal banana pathogens Mycosphaerella fijiensis, Mycosphaerella musicola and Mycosphaerella eumusae

Mf, Zapater; Duchemin, Myriam; Jf, Dussart; D, Coste; Brottier, Philippe; Carlier, Jean

doi:10.1111/j.1755-0998.2008.02180.x

Cited by 19 publications

(24 citation statements)

References 12 publications

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“…() in which Southeast Asia was postulated to be the centre of origin and the source of global migrations of this pathogen. SSR markers were also developed to assess genetic variation in M. musicola (Molina et al ., ; Zapater et al ., ), but no study aimed at elucidating the genetic structure of the pathogen population in Brazil has been conducted using such markers.…”

Section: Introductionmentioning

confidence: 99%

Yellow sigatoka epidemics caused by a panmictic population of Mycosphaerella musicola in Brazil

Gomes

Douhan²,

Lehner

et al. 2017

Plant Pathology

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The genetic structure of Mycosphaerella musicola has never been investigated in Brazil to address epidemiologically related questions associated with yellow sigatoka. A total of 223 single‐conidium isolates from four regions (North, South, Zona da Mata and Triângulo Mineiro) of Minas Gerais State, Brazil, were used to assess the population genetic structure of M. musicola. Isolates were characterized regarding the frequency of the MAT1‐1‐1 or MAT1‐2‐1 idiomorphs and polymorphism at nine microsatellite loci. The mating‐type ratio in three of the four subpopulations was c. 1:1. A total of 87 alleles and 216 multilocus genotypes were identified. The overall population was in linkage equilibrium. Most (93.9%) genetic variation was detected within the subpopulations and there was weak differentiation between them. In total, eight genetic groups were detected and isolates of seven groups were present in all regions. The population of M. musicola in Minas Gerais seems to have high evolutionary potential: it is panmictic and both sexual reproduction and gene flow affect genetic variability. Strategies to avoid fungicide resistance should be enforced and breeding programmes need to consider quantitative resistance in the banana cultivars.

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Section: Introductionmentioning

confidence: 99%

Yellow sigatoka epidemics caused by a panmictic population of Mycosphaerella musicola in Brazil

Gomes

Douhan²,

Lehner

et al. 2017

Plant Pathology

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“…Genotyping was carried out using four PCR multiplex sets comprising 20 microsatellite loci (Zapater et al, 2008;Robert et al, 2010). PCR reaction conditions are also detailed in Robert et al (2010).…”

Section: Molecular Analysismentioning

confidence: 99%

Recent range expansion and agricultural landscape heterogeneity have only minimal effect on the spatial genetic structure of the plant pathogenic fungus Mycosphaerella fijiensis

Rieux

Bellaire²,

et al. 2012

Heredity

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Understanding how geographical and environmental features affect genetic variation at both the population and individual levels is crucial in biology, especially in the case of pathogens. However, distinguishing between these factors and the effects of historical range expansion on spatial genetic structure remains challenging. In the present study, we investigated the case of Mycosphaerella fijiensis-a plant pathogenic fungus that has recently colonized an agricultural landscape characterized by the presence of potential barriers to gene flow, including several commercial plantations in which disease control practises such as the use of fungicides are applied frequently, and low host density areas. We first genotyped 300 isolates sampled at a global scale on untreated plants in two dimensions over a 50 Â 80-km area. Using two different clustering algorithms, no genetic structure was detected in the studied area, suggesting expansion of large populations and/or no influence of potential barriers. Second, we investigated the potential effect of disease control practises on M. fijiensis diversity by comparing populations sampled in commercial vs food-crop plantations. At this local scale, we detected significantly higher allelic richness inside commercial plantations compared with the surrounding food-crop plantation populations. Analysis of molecular variance indicated that 99% of the total genetic variance occurred within populations. We discuss the suggestion that high population size and/or high migration rate between populations might be responsible for the absence of any effect of disease control practises on genetic diversity and differentiation.

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“…The development of simple sequence repeat markers, also known as microsatellites, with 2-5 nucleotide repeats for M. fijiensis, has recently been undertaken (Yang and Zhong, 2008;Zapater et al, 2008); however, genetic diversity or population structure studies using more robust DNA markers are only at the early stages and more DNA markers are still needed (Molina and Kahl, 2004).…”

Section: Resultsmentioning

confidence: 99%

Variable number of tandem repeat markers in the genome sequence of Mycosphaerella fijiensis, the causal agent of black leaf streak disease of banana (Musa spp)

Garcia¹,

Lee²,

Ferreira³

et al. 2010

Genet. Mol. Res.

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ABSTRACT. We searched the genome of Mycosphaerella fijiensis for molecular markers that would allow population genetics analysis of this plant pathogen. M. fijiensis, the causal agent of banana leaf streak disease, also known as black Sigatoka, is the most devastating pathogen attacking bananas (Musa spp). Recently, the entire genome sequence of M. fijiensis became available. We screened this database for VNTR markers. Forty-two primer pairs were selected for validation, based on repeat type and length and the number of repeat units. Five VNTR markers showing multiple alleles were validated with a reference set of isolates from different parts of the world and a population from a banana plantation in Costa Rica. Polymorphism information content values varied from 0.6414 to 0.7544 for the reference set and from 0.0400 and 0.7373 for the population set. Eighty percent of the polymorphism information content values were above 0.60, indicating that the markers are highly informative. These markers allowed robust scoring of agarose gels and proved to be useful for variability and population genetics studies. In conclusion, the strategy we developed to identify and validate VNTR markers is an efficient means to incorporate markers that can be used for fungicide resistance management and to develop breeding strategies to control banana black leaf streak disease. This is the first report of VNTR-minisatellites from the M. fijiensis genome sequence.

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Microsatellite markers for the fungal banana pathogens Mycosphaerella fijiensis, Mycosphaerella musicola and Mycosphaerella eumusae

Cited by 19 publications

References 12 publications

Yellow sigatoka epidemics caused by a panmictic population of Mycosphaerella musicola in Brazil

Yellow sigatoka epidemics caused by a panmictic population of Mycosphaerella musicola in Brazil

Recent range expansion and agricultural landscape heterogeneity have only minimal effect on the spatial genetic structure of the plant pathogenic fungus Mycosphaerella fijiensis

Variable number of tandem repeat markers in the genome sequence of Mycosphaerella fijiensis, the causal agent of black leaf streak disease of banana (Musa spp)

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