Microsatellite analysis indicates the specific genetic basis of Czech bolting garlic

Ovesná, Jaroslava; Leišová‐Svobodová, Leona; Kučera, L.

doi:10.17221/82/2014-cjgpb

Cited by 17 publications

(11 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, the remaining primers showed lower diversity. Ovesna et al [25] also reported that high number of allele was in accordance with high gene diversity in the study aiming to identify of Czech garlic varieties using 14 SSR markers. The number of allele and gene diversity in the present study coincided with Ma et al [26], who found the former between 2 and 9, and the latter between 0.0476 and 0.8304 in the study employing 32 accessions of V. faba and 21 microsatellite markers.…”

Section: Resultsmentioning

confidence: 86%

Genetic diversity in some faba bean (Vicia faba L.) genotypes assessed by simple sequence repeats

Tufan

Erdoğan

2016

Biotechnology & Biotechnological Equipment

View full text Add to dashboard Cite

The success of the breeding programmes depends on the dimension of genetic variability. Simple sequence repeats (SSR) have been extensively used in the studies including genetic characterization of many plant species. The objective of this study was to investigate the genetic variation in 22 faba bean genotypes, 18 of which were originated from International Center for Agricultural Research in the Dry Areas (ICARDA) and 4 of which were cultivated genotypes in Turkey, using SSR markers. Of 41 SSR markers used, 25 produced bands. As a result of SSR amplification, a total of 39 bands, 25 of which were polymorphic and 14 of which were monomorphic, were obtained. The mean gene diversity and polymorphism information content values were 0.27 and 0.24, respectively. The faba bean genotypes cultivated in Turkey had greater genetic diversity than those that originated from ICARDA. The faba bean genotypes FLIP10-and FLIP03-were successfully separated, using the un-weighted pair group method with arithmetic average dendrogram constructed via Jaccard similarity coefficients. These results were further supported by factor analysis substantially. The results indicated that there is sufficient genetic diversity among the tested faba bean genotypes (especially cultivated in Turkey) and could be used in faba bean breeding programmes.

show abstract

Section: Resultsmentioning

confidence: 86%

Genetic diversity in some faba bean (Vicia faba L.) genotypes assessed by simple sequence repeats

Tufan

Erdoğan

2016

Biotechnology & Biotechnological Equipment

View full text Add to dashboard Cite

show abstract

“…The primers are listed in Table 2 and include the annealing temperature and the number of detected alleles per microsatellite locus across the analysed set. PCR with fluorescently labelled primers (6-fam, vic, ned and pet; Life Technologies, Foster City, USA) was performed in a reaction as described by Ovesná et al (2014). The amplification products were separated by capillary electrophoresis in the sequencer ABI PRISM 3130 (Applied Biosystems, Foster City, USA).…”

Section: Methodsmentioning

confidence: 99%

The selection and validation of a marker set for the differentiation of onion cultivars from the Czech Republic

Mitrová¹,

Svoboda²,

Ovesná³

2015

Czech J. Genet. Plant Breed.

Self Cite

View full text Add to dashboard Cite

Mitrová K., Svoboda P., Ovesná J. (2015): The selection and validation of a marker set for the differentiation of onion cultivars from the Czech Republic. Czech J. Genet. Plant Breed., 51: 62-67.The onion (Allium cepa L.) constitutes an important part of the human diet and is of economic importance on the vegetable market. Methods that allow unambiguous differentiation of cultivars and breeding lines are required for market control and the protection of plant breeders' rights. DNA analyses, such as single sequence repeat polymorphism (SSRs), represent such a tool. Based on the evaluation of polymorphism in 21 SSR loci, we established a panel of 15 easy to score SSR markers that differentiated 16 commercial onion cultivars in the Czech Republic. The polymorphism ranged from 2-3 alleles per locus, indicating a low level of diversity among the cultivars. The panel can also be used to evaluate genetic resources.

show abstract

“…The same set of cultivars was subjected to evaluation of DNA variability by the SSR length polymorphism analysis. DNA extraction was performed from leaves as identical results are obtained regardless of the tissue type used for analysis (Ovesná et al 2014). The varieties were clearly identified, and each was assigned a typical DNA profile.…”

Section: Resultsmentioning

confidence: 99%

“…Total genomic DNA was extracted from frozen pooled leaves in parallel using the CTAB (cetyltrimethylammonium bromide) method, as described by Ovesná et al (2014). DNA quality was examined by electrophoresis, and the amount was quantified by spectrophotometry.…”

Section: Methodsmentioning

confidence: 99%

Antioxidant activity, S-alk(en)yl-l-cysteine sulfoxide and polyphenol content in onion (Allium cepa L.) cultivars are associated with their genetic background

Mitrová¹,

Hrbek²,

Svoboda³

et al. 2016

Czech J. Food Sci.

Self Cite

View full text Add to dashboard Cite

The onion (Allium cepa L.) is one of the most valuable vegetables in the world and was known already in ancient times. The onion is one of the classic examples of Allium species used not only for culinary preparations but also for medicinal purposes. Compounds in onions have been reported to exhibit a range of health benefits, including anticancer properties, antiplatelet activity, antithrombotic activity and antibiotic effects (Suleria et al. 2015). These benefits are associated with sulphur compounds called cysteine sulfoxides (Starkenman et al. 2011). Onions also synthesise significant amounts of other metabolites including fructo-oligosaccharides (FOS), flavonoids, and saponins that contribute to their taste and health benefits (Pérez-Gregorio et al. 2014).Several studies have indicated that the secondary metabolite content in Allium vegetables is associated both with environmental factors and with the genetic background of the respective cultivars (Huchette et al. 2005;Crosby et al. 2007). Perner et al. (2008) provided clear evidence that antioxidant activity, quercetin glycosides and organosulphur compounds can be increased in onion plants sufficiently supplied with nitrates or mycorrhizal fungi. Jabbes et al. (2012) described an association between organosulphur compounds and yield. The yield is generally considered to be genotype and environment dependent. Bystricka et al. (2015) observed differences between cultivars and a statistically significant increase of quercetin formation in all cultivars during vegetation. Also Bernaert et al. (2012) reported differences in S-alk(en)yl-l-cysteine sulfoxide (ASCO) content in various garlic cultivars. Lee et al. (2015) showed differences in secondary metabolite content Six onion cultivars Bingo, Dormo, Elenka, Elbrus, Spirit, and Sturon grown in the Czech Republic for commercial purposes were analysed to investigate the content of health-promoting compounds. The results showed that at harvest time, cysteine sulfoxide content varied from 32.38 to 44.16 g/kg of dry weight, polyphenol content was between 2.66 and 3.37 g/kg of dry weight, and antioxidant activity ranged from 0.75 to 0.83 g/kg. Cv. Bingo had the highest level of the analysed compounds. The cultivars were concurrently analysed by DNA (microsatellite) markers. Dendrograms based on the chemical composition and DNA analysis were almost identical. This finding confirms the dependence of the secondary metabolite content on onion genotype. Antioxidant Activity, S-alk(en)yl-l-cysteine

show abstract

Microsatellite analysis indicates the specific genetic basis of Czech bolting garlic

Cited by 17 publications

References 33 publications

Genetic diversity in some faba bean (Vicia faba L.) genotypes assessed by simple sequence repeats

Genetic diversity in some faba bean (Vicia faba L.) genotypes assessed by simple sequence repeats

The selection and validation of a marker set for the differentiation of onion cultivars from the Czech Republic

Antioxidant activity, S-alk(en)yl-l-cysteine sulfoxide and polyphenol content in onion (Allium cepa L.) cultivars are associated with their genetic background

Contact Info

Product

Resources

About