MicroRNAs – targeting and target prediction

Saito, Takaya; Sætrom, Pål

doi:10.1016/j.nbt.2010.02.016

Cited by 117 publications

(92 citation statements)

References 79 publications

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“…To identify miRNAs that potentially bind MyoD, we queried the MicroCosm targets (http://www.ebi.ac.uk/enright-srv/microcosm/) and TargetScan prediction program (http://www.targetscan.org/) based on the zebrafish genome (Alexiou et al, 2011;Rajewsky, 2006;Saito and Saetrom, 2010). We compared the sequence conservation of miRNA target sites between zebrafish and tilapia using the Basic Local Alignment Search Tool (http://blast.ncbi.nlm.nih.gov/Blast.cgi).…”

Section: Prediction Of Myod-binding Mirnasmentioning

confidence: 99%

miR-203b: a novel regulator of MyoD expression in tilapia skeletal muscle

Yan

Guo

Zhu

et al. 2012

Journal of Experimental Biology

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Section: Prediction Of Myod-binding Mirnasmentioning

confidence: 99%

miR-203b: a novel regulator of MyoD expression in tilapia skeletal muscle

Yan

Guo

Zhu

et al. 2012

Journal of Experimental Biology

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“…Even with this limitation, there are typically hundreds to thousands of targets predicted for a single miRNA, and many are shown to be false positives when interrogated by luciferase assays (Bentwich 2005;Rajewsky 2006;Baek et al 2008;Cloonan et al 2008). Along with the lack of overlap between targets predicted by different programs (Saito and Saetrom 2010), this limits streamlined analysis of biologically relevant targets and their subsequent experimental validation.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-182-5p targets a network of genes involved in DNA repair

Krishnan

Steptoe

Martin

et al. 2012

RNA

113

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MicroRNAs are noncoding regulators of gene expression, which act by repressing protein translation and/or degrading mRNA. Many have been shown to drive tumorigenesis in cancer, but functional studies to understand their mode of action are typically limited to single-target genes. In this study, we use synthetic biotinylated miRNA to pull down endogenous targets of miR-182-5p. We identified more than 1000 genes as potential targets of miR-182-5p, most of which have a known function in pathways underlying tumor biology. Specifically, functional enrichment analysis identified components of both the DNA damage response pathway and cell cycle to be highly represented in this target cohort. Experimental validation confirmed that miR-182-5p-mediated disruption of the homologous recombination (HR) pathway is a consequence of its ability to target multiple components in that pathway. Although there is a strong enrichment for the cell cycle ontology, we do not see primary proliferative defects as a consequence of miR-182-5p overexpression. We highlight targets that could be responsible for miR-182-5p-mediated disruption of other biological processes attributed in the literature so far. Finally, we show that miR-182-5p is highly expressed in a panel of human breast cancer samples, highlighting its role as a potential oncomir in breast cancer.

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“…-6mer: Pairing includes six nucleotides at the seed region only, -7mer-A1: Nucleotide pairing at the seed, and miRNA's 1st nucleotide corresponding to the Adenine (A) nucleotide, -7mer-m8: Pairing of miRNA's 8th nucleotide in addition to the nucleotide pairing at the seed, -8mer: Characterized by nucleotide pairing at seed region and miRNA Genes of the Cardiovascular Diseases nucleotide 8 of miRNA, and nucleotide A located against nucleotide 1 of miRNA [17,27].…”

Section: Micro Rna Biogenesismentioning

confidence: 99%

Unknown Aspects of Well-known Cardiovascular Diseases: Identification of Novel miRNA Genes

Çeviker¹,

Bağcı²,

Yazkan³

2016

Ann Clin Anal Med

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ÖzetKodlanmayan küçük ribonükleik asit (miRNA)'lerin bulunması ve işlevlerinin tanımlanmasıyla, RNA'ların canlı yaşamı için çok önemli süreçlerde rol oynadıkları belirlenmiştir. miRNA'larda meydana gelen bozukluklar birçok hastalı-ğa yol açmaktadır. Beklendiği üzere kanserler, nörodejeneratif hastalıklar ve immün yetmezlik gibi hastalıklarla ilişkilendirilmiş ve son yıllarda yeni yapı-lan çalışmalarla kardiyovasküler hastalıklarla olan ilişkilileri gösterilmiştir. Bu miRNA'lar, yeni tedavi yaklaşımlarında hem hedef hem de araç olarak görül-mektedirler. İnsan genomundaki tüm miRNA'ların işlevlerinin aydınlatılmasıy-la yeni tedavi yaklaşımları geliştirilebilecektir. Bu derlemede, miRNA'ların kardiyovasküler hastalıklarla ilişkili olduğu kuvvetli çalışmalarla ispatlanan çeşit-leri, kardiyovasküler hastalıklarla olan ilişkileri, ve tanı-tedavi amaçlı kullanımlarıyla ilgili literatüre dayalı değerlendirme yapılmıştır. Anahtar KelimelerKardiyovasküler Hastalık; Ateroskleroz; Miyokard Enfarktüsü (MI); Mikro RNA

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MicroRNAs – targeting and target prediction

Cited by 117 publications

References 79 publications

miR-203b: a novel regulator of MyoD expression in tilapia skeletal muscle

miR-203b: a novel regulator of MyoD expression in tilapia skeletal muscle

MicroRNA-182-5p targets a network of genes involved in DNA repair

Unknown Aspects of Well-known Cardiovascular Diseases: Identification of Novel miRNA Genes

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