MicroRNAs of rat articular cartilage at different developmental stages identified by Solexa sequencing

Sun, Jian; Zhong, Nan; Li, Q.; Min, Zixin; Zhao, Wenran; Sun, Qi; Tian, Liang; Yu, Hua; Shi, Qi; Zhang, F.; Lu, Shemin

doi:10.1016/j.joca.2011.07.002

Cited by 29 publications

(36 citation statements)

References 43 publications

(38 reference statements)

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“…In previous reports, miR-26a was on the list of the top 10% of miRNAs constitutively expressed at a high level in rat spleen [30], and also found to be considerable abundant in rat articular cartilage using Solexa sequencing from our previous study [31]. Its outstanding sufficiency in major immune organs and cartilage suggests its potential implication in arthritis development.…”

Section: Discussionmentioning

confidence: 81%

MicroRNA-26a negatively regulates toll-like receptor 3 expression of rat macrophages and ameliorates pristane induced arthritis in rats

Jiang

Zhu

et al. 2014

Arthritis Res Ther

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IntroductionAbnormal toll-like receptor (TLR)3 signaling plays an indispensable role in pathogenesis of both experimental and human rheumatoid arthritis, and microRNAs (miRNAs) might orchestrate this signaling pathway. This study was performed to determine the relationship between miR-26a and TLR3 in rat macrophages and to observe effects of miR-26a mimic on pristane induced arthritis (PIA) in rats.MethodsDual luciferase reporter assay was used to validate the direct interaction between miR-26a (a candidate miRNA to target tlr3 mRNA) and tlr3 3′UTR. MiR-26a regulation on TLR3 gene expression was determined using RT-qPCR and Western blotting after miR-26a mimics and inhibitors were transfected into rat macrophage line NR8383 cells. Poly I:C (TLR3 ligand) was used to trigger TLR3 activation, and mRNA expression of its downstream cytokines interferon (ifn)-β and tumor necrosis factor (tnf)-α was accordingly detected to determine the regulation of TLR3 signaling. Expressions of TLR3 and miR-26a were detected during rat bone marrow derived macrophage (BMDM) induction, in pristane stimulated NR8383 cells and spleens from methotrexate (MTX) treated PIA rats. A miR-26a mimic was administrated intraperitoneally to PIA rats, and arthritis severity was evaluated by macroscopic or microscopic observations.ResultsDirect target relationship between miR-26a and tlr3 mRNA in rats was confirmed. Modifications of miR-26a function by transfection of miR-26a mimics and inhibitors exhibited corresponding repression and augmentation of TLR3 and its signaling downstream cytokine expressions in NR8383 cells. The alteration of miR-26a expression was negatively related with TLR3 expression during BMDM induction, in pristane-primed NR8383 cells and PIA rat spleens. Moreover, both abnormal expressions were rescued in MTX treated arthritis rat spleens. The miR-26a mimic treatment displayed the depression of TLR3 expression and ameliorated the disease severity in the rats with pristane induced arthritis.ConclusionsMiR-26a negatively regulates TLR3 signaling via targeting of TLR3 itself in rat macrophages, and this finding provides a novel insight into abnormal TLR3 overexpression during experimental arthritis.

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Section: Discussionmentioning

confidence: 81%

MicroRNA-26a negatively regulates toll-like receptor 3 expression of rat macrophages and ameliorates pristane induced arthritis in rats

Jiang

Zhu

et al. 2014

Arthritis Res Ther

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“…In this context, it is of interest to review a study that identified microRNA profiles of rat articular cartilage at different developmental stages (194). Three small RNA libraries were constructed from the femoral head cartilage of Sprague-Dawley rats at postnatal day 0, day 21 and day 42 and sequenced by a deep sequencing approach.…”

Section: New Perspective Targets For Temporal Modulation In Cartilmentioning

confidence: 99%

Time-Dependent Processes in Stem Cell-Based Tissue Engineering of Articular Cartilage

Gadjanski

Spiller

Vunjak-Novakovic

2011

Stem Cell Rev and Rep

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Articular cartilage (AC), situated in diarthrodial joints at the end of the long bones, is composed of a single cell type (chondrocytes) embedded in dense extracellular matrix comprised of collagens and proteoglycans. AC is avascular and alymphatic and is not innervated. At first glance, such a seemingly simple tissue appears to be an easy target for the rapidly developing field of tissue engineering. However, cartilage engineering has proven to be very challenging. We focus on time-dependent processes associated with the development of native cartilage starting from stem cells, and the modalities for utilizing these processes for tissue engineering of articular cartilage.

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“…There are several advanced methods to study the profile of miRNAs in OA from human and animals such as microarray, Solexa-based deep sequencing and next generation sequencing (NGS) [37-39]. Recently, Dr. Desjardin showed 609 and 622 miRNAs expression profiles in cartilage and bone, respectively, including 282 and 293 putative new miRNAs in cartilage and bone from equine [38].…”

Section: Resultsmentioning

confidence: 99%

“…Dr. Sun identified expression profiles of miRNAs of articular cartilage from rat femoral head cartilage. They made it possible to highlight 310 known miRNAs including 86 novel miRNAs candidates [39]. Current, we used NGS to analysis the miRNA expression profiles from articular cartilage and subchondral bone in normal, OA and OA+shockwave (SW).…”

Section: Resultsmentioning

confidence: 99%

Next-generation sequencing identifies articular cartilage and subchondral bone miRNAs after ESWT on early osteoarthritis knee

Cheng

Wang

et al. 2016

Oncotarget

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Extracorporeal shockwave therapy (ESWT) has shown chondroprotective effects on the initiation of the osteoarthritis (OA) changes of the rat knee. This study evaluated 69 significant expressed profiles of microRNA (miRNA) in the articular cartilage and subchondral bone after ESWT. There were 118 target genes identified for miRNAs of interest in articular cartilage and 214 target genes in subchondral bone by next generation sequencing (NGS). In principal component analysis (PCA), the relationships of miRNA expression in bone and cartilage were improved after ESWT. Global functional annotation showed that predicted targets were involved in cartilage development, inflammatory and immune response, ion binding, angiogenesis, cell adhesion, cell cycle, transcription and translation, gene expression, NTP binding, signal transduction, collagen fibril organization, apoptotic process, chondrocyte differentiation, cell differentiation, bone development as well as cell proliferation. The miRNAs profile and the target genes were comprehensively surveyed and compared in articular cartilage and subchondral bone of early OA knee before and after ESWT. Our study represents the direct assessment to date of miRNA expression profiling in early OA articular cartilage and subchondral bone. The results provide insights that could contribute to the development of new biomarkers and therapeutic strategies for OA changes and the treatment with ESWT.

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MicroRNAs of rat articular cartilage at different developmental stages identified by Solexa sequencing

Abstract: Our study provided a unique opportunity to decipher how the elaboration of the miRNA repertoire contributes to the development process of articular cartilage.

Cited by 29 publications

References 43 publications

MicroRNA-26a negatively regulates toll-like receptor 3 expression of rat macrophages and ameliorates pristane induced arthritis in rats

MicroRNA-26a negatively regulates toll-like receptor 3 expression of rat macrophages and ameliorates pristane induced arthritis in rats

Time-Dependent Processes in Stem Cell-Based Tissue Engineering of Articular Cartilage

Next-generation sequencing identifies articular cartilage and subchondral bone miRNAs after ESWT on early osteoarthritis knee

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