MicroRNA modulation in complex regional pain syndrome

Orlova, I. A.; Alexander, Guillermo M.; Qureshi, Rehman; Saçan, Ahmet; Graziano, Alessandro; Barrett, James E.; Schwartzman, Robert J.; Ajit, Seena K.

doi:10.1186/1479-5876-9-195

Cited by 147 publications

(135 citation statements)

References 43 publications

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“…n ¼ 6-8 per group. previous reports [10], suggesting that suppression of proinflammatory cytokine activity could be a promising clinical approach.…”

Section: Discussionmentioning

confidence: 99%

Circulating microRNA expression profile: a novel potential predictor for chronic nervous lesions

Xu¹,

Zhang²,

Pu³

et al. 2014

ABBS

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The mechanisms of chronic neuropathic pain are not clear. Serum microRNAs (miRNAs) might show a special feature for chronic nervous lesions. However, little is known about the changes in circulating miRNAs for the neuropathic pain. Therefore, changes in the circulating miRNAs expression profile for the neuropathic pain were investigated. Serum was collected from rats before and after spinal nerve ligation (SNL) surgery, and a microarray analysis was performed to determine the changes in miRNA expression profile. The expression of inflammatory cytokines in serum from the same individuals, including interleukin-6 (IL-6), tumor necrosis factor-a (TNF-a), and monocyte chemotactic protein-1 (MCP-1), was also measured. The results showed that the expression levels of IL-6, TNF-a, and MCP-1 were significantly elevated in SNL rats which were significantly correlated with pain levels. Nine miRNAs with significantly different expression levels before and after SNL surgery were identified by microarray analysis, which were further validated by quantitative real-time polymerase chain reaction analyses. Compared with naive rats without SNL surgery, the expression of five miRNAs (hsa-miR-221, hsa-miR-34c, hsa-miR-21, hsa-miR-30a-5p, and hsa-miR-206) in the serum of rats after SNL surgery was decreased and four miRNAs (hsa-miR-31-5p, hsa-miR-133b, hsamiR-22, and hsa-miRPlus-A1087) were increased, suggesting that miRNA changes may involve in the regulation of neuropathic pain. TargetScan was used to predict mRNA targets for these miRNAs, and the results showed that the transcripts with multiple predicted target sites belonged to neurologically important pathways. Bioinformatics analysis revealed that several target genes are related to the activation of cell signaling associated with nervous lesions. In this study, the changes to miRNA profiles in serum under neuropathic pain conditions were shown for the first time, suggesting that circulating miRNAs profile in serum is a potential predictor for neuropathic pain.

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“…n ¼ 6-8 per group. previous reports [10], suggesting that suppression of proinflammatory cytokine activity could be a promising clinical approach.…”

Section: Discussionmentioning

confidence: 99%

Circulating microRNA expression profile: a novel potential predictor for chronic nervous lesions

Xu¹,

Zhang²,

Pu³

et al. 2014

ABBS

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“…Leinders et al, 2016 37 recently found that several systemic mirco-RNAs (miR), specifically miR-103, miR-151, and miR-let-7d, were downregulated in patients with fibromyalgia whereas cutaneous miR-let-7d was elevated in patients with reduced small nerve fiber density. miR-let7d has previously been found to be associated with chronic pain in patients with complex regional pain syndrome 38 and to affect the endogenous opioid system 39 , which is known to be impaired in, e.g. KOA 15 or fibromyalgia 40 .…”

Section: Thermal Testing In Knee Osteoarthritismentioning

confidence: 99%

The Role of Preoperative Radiologic Severity, Sensory Testing, and Temporal Summation on Chronic Postoperative Pain Following Total Knee Arthroplasty

Petersen

Simonsen

Laursen

et al. 2018

The Clinical Journal of Pain

115

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Objectives: Knee osteoarthritis (KOA) can be associated with local and central sensitization. As an indicator of the central gain, facilitated temporal summation of pain (TSP) has been found in KOA patients. This facilitation is predictive of the development of chronic postoperative pain after total knee arthroplasty (TKA). Other studies have suggested hypoesthesia/hypoalgesia to thermal stimuli as a feature in KOA. This study investigated associations between preoperative TSP, thermal sensitivity, and radiologic severity for the development of chronic postoperative pain after TKA. Methods: Radiologic KOA (Kellgren and Lawrence), TSP, and thermal stimuli were collected, preoperatively. Clinical knee pain intensity (VAS 0-10) was assessed before and 12 months following TKA. Patients were categorized into a chronic postoperative pain group if they experienced <30% pain reduction of the initial pain after 12 months. Results: In total, 19% of the patients were categorized as chronic pain patients and presented facilitated preoperative TSP (P<0.05) and a trend towards increased heat pain threshold (P=0.077) compared with patients with normal recovery. Pearson correlations found that preoperative TSP (R=0.193; P=0.013), Kellgren and Lawrence (R=−0.168; P=0.027), warm detection threshold (R=0.195; P=0.012), and heat pain threshold (R=0.196; P=0.012) were associated with pain intensity 12 months after TKA where TSP was identified as an independent factor. Discussion: This study showed that preoperatively facilitated TSP in KOA patients was predictive of the development of chronic postoperative pain following TKA. Furthermore, this study is the first to find an association between preoperative hypoalgesia to heat and the development of chronic postoperative pain following TKA.

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“…When studying gene expression profiles in common conditions with subclinical histopathology, such as IBS, high sensitivity and accuracy (reproducibility) are needed, as perturbations in gene expression profiles are often subtle [1][2][3] . miRNAs have been identified as both diagnostic and functional targets in IBS 4,5 , and we are particularly interested in assessing their use as circulating biomarkers of IBS-associated biological perturbations 3,4,6,7 . The clinical utilization of circulating biomarkers is of current interest due to the ease and minimally invasive nature of the collection of the biomarkers' source (e.g., peripheral blood) from patients.…”

Section: Introductionmentioning

confidence: 99%

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

Fourie

Peace

Abey

et al. 2016

JoVE

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The gene expression platform assay allows for robust and highly reproducible quantification of the expression of up to 800 transcripts (mRNA or miRNAs) in a single reaction. The miRNA assay counts transcripts by directly imaging and digitally counting miRNA molecules that are labeled with color-coded fluorescent barcoded probe sets (a reporter probe and a capture probe). Barcodes are hybridized directly to mature miRNAs that have been elongated by ligating a unique oligonucleotide tag (miRtag) to the 3' end. Reverse transcription and amplification of the transcripts are not required. Reporter probes contain a sequence of six color positions populated using a combination of four fluorescent colors. The four colors over six positions are used to construct a gene-specific color barcode sequence. Post-hybridization processing is automated on a robotic prep station. After hybridization, the excess probes are washed away, and the tripartite structures (capture probe-miRNA-reporter probe) are fixed to a streptavidin-coated slide via the biotin-labeled capture probe. Imaging and barcode counting is done using a digital analyzer. The immobilized barcoded miRNAs are visualized and imaged using a microscope and camera, and the unique barcodes are decoded and counted. Data quality control (QC), normalization, and analysis are facilitated by a custom-designed data processing and analysis software that accompanies the assay software. The assay demonstrates high linearity over a broad range of expression, as well as high sensitivity. Sample and assay preparation does not involve enzymatic reactions, reverse transcription, or amplification; has few steps; and is largely automated, reducing investigator effects and resulting in high consistency and technical reproducibility. Here, we describe the application of this technology to identifying circulating miRNA perturbations in irritable bowel syndrome. Video LinkThe video component of this article can be found at

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MicroRNA modulation in complex regional pain syndrome

Cited by 147 publications

References 43 publications

Circulating microRNA expression profile: a novel potential predictor for chronic nervous lesions

Circulating microRNA expression profile: a novel potential predictor for chronic nervous lesions

The Role of Preoperative Radiologic Severity, Sensory Testing, and Temporal Summation on Chronic Postoperative Pain Following Total Knee Arthroplasty

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

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