MicroRNA in pancreatic cancer

Yonemori, Keiichi; Kurahara, Hiroshi; Maemura, Kosei

doi:10.1038/jhg.2016.59

Cited by 138 publications

(139 citation statements)

References 128 publications

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“…This panel was designed and validated with the intent to be incorporated into the routine clinical testing. Table 1 not only summarizes the role and biological function of each miRNA present in this panel but also describes their clinical relevance [16,33,34,42,43]. miRNA is obtained using the miRNeasy FFPE Kit (Qiagen, Hilden, Germany), and it requires four to six FFPE (formalin-fixed, paraffin-embedded) unstained slides of 5 μm each.…”

Section: Mirna and Pancreatic Diseases: The Dartmouth-hitchcock Medicmentioning

confidence: 99%

miRNA analysis in pancreatic cancer: the Dartmouth experience

Abreu

Tsongalis

2017

Clinical Chemistry and Laboratory Medicine (CCLM)

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Pancreatic cancer is considered one of the most lethal cancers being the fourth leading cause of cancer deaths in adults in the United States because of the lack of early signs and symptoms and the lack of early detection. Pancreatic ductal adenocarcinoma (PDAC) is the most common histological type among pancreatic cancers, representing 80%-90% of all solid tumors of the pancreas. The majority of PDAC develops from three precursor lesions: pancreatic intraepithelial neoplasia, intraductual papillary mucinous neoplasm and mucinous cystic neoplasm.

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Section: Mirna and Pancreatic Diseases: The Dartmouth-hitchcock Medicmentioning

confidence: 99%

miRNA analysis in pancreatic cancer: the Dartmouth experience

Abreu

Tsongalis

2017

Clinical Chemistry and Laboratory Medicine (CCLM)

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“…All these modes of actions constitute a complicated, multi-node and multi-layer regulatory network. In addition to its normal functions, assembling evidences suggest that miRNA are also involved in various pathological conditions such as cardiomyopathies [8], atherosclerosis [9], obesity [10] and cancer [11]. Most importantly, emerging evidences suggest that miRNAs play crucial roles in inflammation responses [12], as well as in acute lung injury and ARDS [13].…”

Section: Introductionmentioning

confidence: 99%

NF-κB-Induced MicroRNA-211 Inhibits Interleukin-10 in Macrophages of Rats with Lipopolysaccharide-Induced Acute Respiratory Distress Syndrome

Wang

Chen

et al. 2018

Cell Physiol Biochem

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Background/Aims: The present study addressed the potential involvement of microRNAs in acute respiratory distress syndrome (ARDS)-related inflammation and elucidates the underlying molecular mechanism. Methods: ARDS rat model was established by lipopolysaccharide, with compromised gas exchange capacity and lung edema. The inflammatory cells from bronchoalveolar lavage fluid (BALF) were profiled with automatic blood cell analyzer. The relative fluorescence intensity of BALF-derived macrophages was analyzed by flow cytometry. The relative microRNA expression was determined using microarray and Taqman assay. The secretory interleukin (IL)-10 was measured by enzyme-linked immunosorbent assay. Luciferase reporter assay was performed to determine the regulatory effects of miR-211 and NF-κB on IL-10 and miR-211 expressions, respectively. Chromatin immunoprecipitation (ChIP) was conducted to detect the direct binding of NK-κB on miR-211 promoter. The protein level was determined by Western blot. Results: The provoked acute inflammation was characterized with increased total cells, macrophages, neutrophils and lymphocytes. The relative expression of miR-211 was aberrantly up-regulated in BALF-derived macrophages from ARDS rats, which was accompanied with reduction of secretory IL-10. We further demonstrated that miR-211 inhibited IL-10 expression by binding to its 3’-UTR. The expression of miR-211 was modulated by NF-κB. Conclusion: Here we elucidated a crucial role of NF-κB/miR-211/IL-10 signaling axis in ARDS-related inflammation.

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“…Therefore, there is an urgent requirement for novel therapeutic treatments for patients with pancreatic cancer. A number of studies have indicated that miRNAs contribute to the carcinogenesis and progression of numerous types of human cancer via targeting multiple mRNAs (22)(23)(24). Therefore, identification of specific miRNAs may provide therapeutic implications and may be exploited to improve treatments for patients with pancreatic cancer.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-452 suppresses pancreatic cancer migration and invasion by directly targeting B-cell-specific Moloney murine leukemia virus insertion site 1

Li¹,

Wu²,

Li³

2017

Oncology Letters

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Abstract. Pancreatic cancer, one of the most common cancers globally, is the fourth most common cause of cancer-associated mortality in the USA. The 5-year relative survival rate for patients with pancreatic cancer is ~5% and the median survival time is only 6 months. The poor prognosis is mainly due to early and aggressive local invasion and metastasis, as well as dissemination of the pancreatic cancer cells. The present study demonstrated that microRNA-452 (miR-452) was markedly downregulated in pancreatic cancer tissues, particularly in metastatic tumors and pancreatic cancer cell lines. Overexpression of miR-452 significantly inhibited migration and invasion in pancreatic cancer cells. In addition, the molecular mechanism underlying the inhibitory functions of miR-452 in pancreatic cancer was also investigated. The results indicated that B-cell-specific Moloney murine leukemia virus insertion site 1 (BMI1) was a direct target gene of miR-452 in pancreatic cancer. Overexpression of miR-452 inhibited the migration and invasion of pancreatic cancer, at least partially by knockdown of BMI1 expression. The results provided novel insight with potential therapeutic applications for the treatment of metastatic pancreatic cancer.

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MicroRNA in pancreatic cancer

Cited by 138 publications

References 128 publications

miRNA analysis in pancreatic cancer: the Dartmouth experience

miRNA analysis in pancreatic cancer: the Dartmouth experience

NF-κB-Induced MicroRNA-211 Inhibits Interleukin-10 in Macrophages of Rats with Lipopolysaccharide-Induced Acute Respiratory Distress Syndrome

MicroRNA-452 suppresses pancreatic cancer migration and invasion by directly targeting B-cell-specific Moloney murine leukemia virus insertion site 1

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