microRNA Expression in Women With and Without Polycystic Ovarian Syndrome Matched for Body Mass Index

Butler, Alexandra E.; Ramachandran, Vimal; Sathyapalan, Thozhukat; David, Rachel; Gooderham, Nigel J.; Benurwar, Manasi; Dargham, Soha R.; Hayat, Shahina; Najafi‐Shoushtari, S. Hani; Atkin, Stephen L.

doi:10.3389/fendo.2020.00206

Cited by 25 publications

(18 citation statements)

References 43 publications

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“…In recent years, more and more studies have investigated miRNA expression in women with PCOS ( Santamaria and Taylor, 2014 ; Butler et al, 2020 ; Gebremedhn et al, 2021 ). It has been reported that there are a variety of miRNAs in human follicular fluid, including miR-483-5p, miR-132, miR-320, miR-520c-3p, miR-24 and miR-222, which are related to estrogen secretion.…”

Section: Non-coding Rna In Reproductive Diseasesmentioning

confidence: 99%

Roles of Noncoding RNA in Reproduction

Wang

Gao

et al. 2021

Front. Genet.

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The World Health Organization predicts that infertility will be the third major health threat after cancer and cardiovascular disease, and will become a hot topic in medical research. Studies have shown that epigenetic changes are an important component of gametogenesis and related reproductive diseases. Epigenetic regulation of noncoding RNA (ncRNA) is appropriate and is a research hotspot in the biomedical field; these include long noncoding RNA (lncRNA), microRNA (miRNA), and PIWI-interacting RNA (piRNA). As vital members of the intracellular gene regulatory network, they affect various life activities of cells. LncRNA functions as a molecular bait, molecular signal and molecular scaffold in the body through molecular guidance. miRNAs are critical regulators of gene expression; they mainly control the stability or translation of their target mRNA after transcription. piRNA functions mainly through silencing genomic transposable elements and the post-transcriptional regulation of mRNAs in animal germ cells. Current studies have shown that these ncRNAs also play significant roles in the reproductive system and are involved in the regulation of essential cellular events in spermatogenesis and follicular development. The abnormal expression of ncRNA is closely linked to testicular germ cell tumors, poly cystic ovary syndrome and other diseases. This paper briefly presents the research on the reproductive process and reproductive diseases involving ncRNAs.

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Section: Non-coding Rna In Reproductive Diseasesmentioning

confidence: 99%

Roles of Noncoding RNA in Reproduction

Wang

Gao

et al. 2021

Front. Genet.

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“…Sprague Dawley (SD) rats were treated with PSSS at doses of 0.333, 0.667, or 1 g/kg for 30 days after fed with Lieber-Decarli alcohol liquid diet and CCl4 injection. PSSS treatment improved histopathological lesions in the liver and decreased the serum levels of liver fibrosis indicators (hyaluronan [HA], laminin [LM], and hydroxyproline [ 67 ]). Further, the decreased expression of hepatic MMP-13 and the increased expression of hepatic tissue inhibitor of metalloproteinase (TIMP)-1 was reversed by PSSS administration in mice.…”

Section: Liver Diseasesmentioning

confidence: 99%

Overview of Salvia miltiorrhiza as a Potential Therapeutic Agent for Various Diseases: An Update on Efficacy and Mechanisms of Action

et al. 2020

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Salvia miltiorrhiza Bunge (S. miltiorrhiza) is a medicinal herb that has been used for the treatment for various diseases such as cardiovascular and cerebrovascular diseases in East Asia including Korea. Considering its extensive usage as a therapeutic agent for multiple diseases, there is a need to review previous research regarding its therapeutic benefits and their mechanisms. Therefore, we searched PubMed and PubMed Central for articles reporting its therapeutic effects on certain disease groups including cancers, cardiovascular, liver, and nervous system diseases. This review provides an overview of therapeutic benefits and targets of S. miltiorrhiza, including inflammation, fibrosis, oxidative stress, and apoptosis. The findings on multi-functional properties of S. miltiorrhiza discussed in this article support the efficacy of S. miltiorrhiza extract on various diseases, but also call for further research on the multiple mechanisms that mediate its therapeutic effects.

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“…Let-7i expression was decreased in atretic follicles as compared to that in healthy follicles (23) and was signi cantly up-regulated in follicle activation (24). A recent study showed that the expression of let-7i was signi cantly different between PCOS patients and controls (25,26), that suggested the potential role of let-7i in the pathogenesis of PCOS.…”

Section: Introductionmentioning

confidence: 99%

Let-7i Regulates KGN Proliferation and Estradiol Biosynthesis By Directly Targeting IMP2 in Polycystic Ovary Syndrome

Shen

et al. 2021

Preprint

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Background: Increased granulosa cell division is associated with abnormal folliculogenesis in polycystic ovary syndrome (PCOS). As the most abundant microRNA molecule in the development of follicles, let-7i was found to be differentially expressed in PCOS patients and controls. This study aimed to investigate the role of let-7i in PCOS and explore its related mechanisms.Methods: The expression of let-7i was measured in GCs from women with PCOS and without PCOS. An immortalized human granulose cell line, KGN, was used for the functional study. Let-7i mimics, let-7i inhibitors, lentiviruses expressing IMP2 and small-interfering RNA were transfected respectively into KGN cells. KGN cell proliferation was determined by an EdU assay kit and cck-8. Cell cycle and apoptosis were determined by PI staining and flow cytometry analysis. Quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting were utilized to evaluate genes expression. Estradiol biosynthesis was determined by ELISA analysis. Bioinformatics analysis and luciferase reporter assay were applied to confirm the target gene of let-7i.Results: let-7i was down-regulated in PCOS GCs. Let-7i mimics (150 nM) inhibited KGN proliferation, arrested cell cycle progression, and decreased aromatase expression and estradiol production, while the let-7i inhibitors (150 nM) had the opposite effect. Bioinformatics analysis and qRT-PCR identified IMP2 was a target of let-7i. QRT-PCR and western blot analysis indicated that IMP2 was up-regulated in PCOS GCs and the expression of IMP2 was suppressed by let-7i in KGN. The further luciferase reporter assay combined with rescue assay validated that let-7i inhibited KGN proliferation and estradiol production by directly targeting IMP2 mRNA.Conclusions: Let-7i was down-regulated in PCOS GCs. Let-7i overexpression inhibited KGN proliferation and decreased estradiol production in an IMP2-dependent manner, providing insights into the pathogenesis of PCOS.

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microRNA Expression in Women With and Without Polycystic Ovarian Syndrome Matched for Body Mass Index

Cited by 25 publications

References 43 publications

Roles of Noncoding RNA in Reproduction

Roles of Noncoding RNA in Reproduction

Overview of Salvia miltiorrhiza as a Potential Therapeutic Agent for Various Diseases: An Update on Efficacy and Mechanisms of Action

Let-7i Regulates KGN Proliferation and Estradiol Biosynthesis By Directly Targeting IMP2 in Polycystic Ovary Syndrome

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