MicroRNA as a Therapeutic Target in Cardiac Remodeling

Chen, Chao; Ponnusamy, Murugavel; Liu, Cuiyun; Gao, Jinling; Wang, Kun; Li, Peifeng

doi:10.1155/2017/1278436

Cited by 72 publications

(66 citation statements)

References 197 publications

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“…Next, we sought to identify miR-27b targets and the potential physiological relevance of E2- and BPA-induced regulation of miR-27b. This particular miR was chosen because (i) the magnitude of suppression and time course of suppression at 8 and 16 h by E2 and BPA were similar and (ii) miR-27b has been shown to be important for other tissues that undergo significant remodeling and vascularization ( Kang et al 2013 , Chen et al 2017 b ). TargetScan ( www.targetscan.org ) was used to screen potential miR-27b targets.…”

Section: Resultsmentioning

confidence: 99%

ESTROGEN-REGULATED miRNA-27b IS ALTERED BY BISPHENOL A IN ENDOMETRIAL STROMAL CELLS

Reed

Babayev²,

Chen

et al. 2018

Reproduction

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MicroRNAs (miRs) are small molecules important for regulation of transcription and translation. The objective was to identify hormonally regulated miRs in human endometrial stromal cells and to determine the impact of the endocrine disruptor, bisphenol A (BPA), on those miRs. miR microarray analysis and multiple confirmatory cell preparations treated with 17β-estradiol (E2) and BPA altered miR-27b, let-7c, let-7e and miR-181b. Further, decidualization downregulated miR-27b. VEGFB and VEGFC were validated as targets of miR-27b. Identification of miR-27b target genes suggests that BPA and E2 downregulate miR-27b thereby leading to upregulation of genes important for vascularization and angiogenesis of the endometrium during the menstrual cycle and decidualization.

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Section: Resultsmentioning

confidence: 99%

ESTROGEN-REGULATED miRNA-27b IS ALTERED BY BISPHENOL A IN ENDOMETRIAL STROMAL CELLS

Reed

Babayev²,

Chen

et al. 2018

Reproduction

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“…miR-199a-5p is localized in the introns of the dynamin genes and is expressed in cancer, brain, liver and cardiovascular system, particularly in cardio myocytes and endothelial cells [25,26]. It is also associated with cancer and cardiovascular diseases [27,28]. miR-199a-5p decreased in cardio myocytes exposed to hypoxia [27, 29[.…”

Section: Discussionmentioning

confidence: 99%

Effective microRNAs in Ischemia/Reperfusion Before and After Bypass Graft Surgery in Coronary Artery Patients

Kandilli¹,

Şg²,

Yardımcı³

et al. 2020

Preprint

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BackgroundCardiovascular diseases (CVD) are among the causes of morbidity and mortality in the world. Significant advances have been made in the diagnosis, treatment and prognosis of CVD. New biomarkers and therapeutic targets are needed to reduce the incidence of this disease. Recently, there is growing evidence that circulating microRNAs can be used as diagnostic biomarkers in this disease. Methods We compared five microRNA (hsa-miR-21-5p, hsa-mi181a-5p, hsa-miR-199a-5p, hsa-miR-199b-5p and hsa-miR-320a) expression levels associated with ischemia/reperfusion before and after bypass graft surgery in serum samples of patients (N=46) with coronary artery disease and healthy control subjects (N=48). Expression measurements were made for each miRNA preoperatively and postoperatively at 1. and 24. hours, and then compared with the control subjects. Troponin I, creatine phosphate kinase and creatine kinase myocardial band cardiac markers were measured before and 1 and 24 hours postoperatively and compared to miRNA expressions and controls. Quantitative real-time PCR was used for expression analysis. The data were analyzed by Mann-Whitney test, chi-squared test, Logistic Regression analysis, and Kruskal-Wallis test with the statistical package SPSS. Results The five miRNAs were down-regulated compared to controls. The expression level for miR-199a at 24 h postoperatively was significantly lower than at 1 h (p=0.001). Receiver operating characteristic analysis showed that the area under the curve of miR-199a-5p was 0.810 (sensitivity 87% and specificity 68.5%) in preoperative patients. Conclusions: miR-199a and miR-199b in serum are a novel non-invasive biomarker candidate for coronary artery disease.

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“…miRNAs have recently become a focus of research. Their main function is post-transcriptional gene regulation in plants and animals (32)(33)(34), and studies have increasingly focused on their function in heart development (35)(36)(37). We identified previously that miR-29c-3p is upregulated in the serum of pregnant women carrying fetuses with CHD (9).…”

Section: Discussionmentioning

confidence: 99%

Akt3 is a target of miR-29c-3p and serves an important function in the pathogenesis of congenital heart disease

Chen

et al. 2018

Int J Mol Med

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Our previous studies identified that the expression of microRNA-29c (miR-29c-3p) was significantly increased in the serum of pregnant women carrying fetuses with congenital heart disease (CHD) compared with in that of normal pregnant women. However, the mechanism by which miR-29c-3p affects development of the embryonic heart remained unclear. The aim of the present study was to investigate the effect and potential molecular mechanism of miR-29c-3p overexpression on P19 cell proliferation, apoptosis and differentiation. miR-29c-3p-overexpression and protein kinase Bγ (Akt3)-knockdown cell lines were constructed using transfection technology. The function of miR-29c-3p and Akt3 in cardiomyocyte development was investigated by determining the proliferation, apoptosis and differentiation of P19 cells, which can differentiate into cardiomyocytes induced by dimethylsulfoxide. Bioinformatic analysis and luciferase assays were performed to explore the association between Akt3 and miR-29c-3p. The results of the present study revealed that miR-29c-3p overexpression and Akt3 knockdown suppressed proliferation, and promoted apoptosis and differentiation in P19 cells. Akt3 was also demonstrated to be a target of miR-29c-3p. Therefore, overexpression of miR-29c-3p may inhibit proliferation, and promote apoptosis and differentiation in P19 cells by inhibiting the expression of Akt3. miR-29c-3p may be a potential therapeutic target for the treatment of CHD.

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MicroRNA as a Therapeutic Target in Cardiac Remodeling

Cited by 72 publications

References 197 publications

ESTROGEN-REGULATED miRNA-27b IS ALTERED BY BISPHENOL A IN ENDOMETRIAL STROMAL CELLS

ESTROGEN-REGULATED miRNA-27b IS ALTERED BY BISPHENOL A IN ENDOMETRIAL STROMAL CELLS

Effective microRNAs in Ischemia/Reperfusion Before and After Bypass Graft Surgery in Coronary Artery Patients

Akt3 is a target of miR-29c-3p and serves an important function in the pathogenesis of congenital heart disease

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