MicroRNA-323-3p inhibits cell invasion and metastasis in pancreatic ductal adenocarcinoma<i>via</i>direct suppression of SMAD2 and SMAD3

Wang, Chunyou; Liu, Pian; Wu, Heshui; Cui, Pengfei; Li, Yongfeng; Liu, Yao; Liu, Zhiqiang; Gou, Shanmiao

doi:10.18632/oncotarget.7482

Cited by 58 publications

(56 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consistent with this model, suppression of miR-323a-3p augments and overexpression attenuates wound closure of a monolayer of cells (39). These findings imply that miR-323a-3p downregulation is necessary for the wound healing process.…”

Section: Discussionsupporting

confidence: 72%

miR-323a-3p regulates lung fibrosis by targeting multiple profibrotic pathways

Ge¹,

Habiel²,

Hansbro³

et al. 2016

JCI Insight

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 72%

miR-323a-3p regulates lung fibrosis by targeting multiple profibrotic pathways

Ge¹,

Habiel²,

Hansbro³

et al. 2016

JCI Insight

View full text Add to dashboard Cite

“…SMADs can integrate multiple signaling pathways and regulate the expression of target genes in transforming growth factor‐β‐activated cells . In PC cells, SMAD2 overexpression could increase proliferation and invasion . Identification of the upstream regulators of SMAD2 will help elucidate its critical role in tumorigenesis.…”

Section: Discussionmentioning

confidence: 99%

Silencing of DLEU2 suppresses pancreatic cancer cell proliferation and invasion by upregulating microRNA‐455

Gong

et al. 2019

Cancer Science

View full text Add to dashboard Cite

Long noncoding RNA (lnc RNA ) DLEU 2 has been shown to be dysregulated in several type of tumor. However, the potential biological roles and molecular mechanisms of DLEU 2 in pancreatic cancer ( PC ) progression are poorly understood. In this study, we found that the DLEU 2 level was substantially upregulated in PC tissues and PC cell lines, and significantly associated with poor clinical outcomes in PC patients. Overexpression of DLEU 2 significantly induced PC cell proliferation and invasion, whereas knockdown of DLEU 2 impaired cell proliferation and invasion in vitro. Furthermore, bioinformatics analysis, luciferase reporter assay, and RNA immunoprecipitation assay revealed that DLEU 2 directly bond to microRNA‐455 (miR‐455) and functioned as an endogenous sponge for miR‐455, which could remarkably suppress cell growth and invasion. We also determined that SMAD 2 was a direct target of miR‐455, and the restoration of SMAD 2 rescued cell growth and invasion that were reduced by DLEU 2 knockdown or miR‐455 overexpression. In addition, low miR‐455 expression and high SMAD 2 expression was correlated with poor patient survival. These results indicate that DLEU 2 is an important promoter of PC development, and targeting the DLEU 2/miR‐455/ SMAD 2 pathway could be a promising therapeutic approach in the treatment of PC .

show abstract

“…In this study, luciferase reporter assays demonstrated that miR‐323‐3p directly regulated Smad2 in myoblast differentiation, and our research showed that overexpression of miR‐323‐3p restrained Smad2 expression. Interestingly, Smad2 was associated with miR‐323‐3p in another study . Besides, Smad2 is connected with the TGF‐β signal pathway, which regulates multiple cellular processes, such as cell proliferation and differentiation .…”

Section: Discussionmentioning

confidence: 92%

MicroRNA‐323‐3p promotes myogenesis by targeting Smad2

Qin

Sun

Liu

et al. 2019

J of Cellular Biochemistry

View full text Add to dashboard Cite

Skeletal muscle is an important and complex organ with multiple biological functions in humans and animals. Proliferation and differentiation of myoblasts are the key steps during the development of skeletal muscle. MicroRNA (miRNA) is a class of 21‐nucleotide noncoding RNAs regulating gene expression by combining with the 3′‐untranslated region of target messenger RNA. Many studies in recent years have suggested that miRNAs play a critical role in myogenesis. Through high‐throughput sequencing, we found that miR‐323‐3p showed significant changes in the longissimus dorsi muscle of Rongchang pigs in different age groups. In this study, we discovered that overexpression of miR‐323‐3p repressed myoblast proliferation and promoted differentiation, whereas the inhibitor of miR‐323‐3p displayed the opposite results. Furthermore, we predicted Smad2 as the target gene of miR‐323‐3p and found that miR‐323‐3p directly modulated the expression level of Smad2. Then luciferase reporter assays verified that Smad2 was a target gene of miR‐323‐3p during the differentiation of myoblasts. These findings reveal that miR‐323‐3p is a positive regulator of myogenesis by targeting Smad2. This provides a novel mechanism of miRNAs in myogenesis.

show abstract

MicroRNA-323-3p inhibits cell invasion and metastasis in pancreatic ductal adenocarcinomaviadirect suppression of SMAD2 and SMAD3

Cited by 58 publications

References 47 publications

miR-323a-3p regulates lung fibrosis by targeting multiple profibrotic pathways

miR-323a-3p regulates lung fibrosis by targeting multiple profibrotic pathways

Silencing of DLEU2 suppresses pancreatic cancer cell proliferation and invasion by upregulating microRNA‐455

MicroRNA‐323‐3p promotes myogenesis by targeting Smad2

Contact Info

Product

Resources

About