MicroRNA-24 promotes 3T3-L1 adipocyte differentiation by directly targeting the MAPK7 signaling

Jin, Min; Wu, Yutao; Wang, Jing; Chen, Jian; Huang, Yi‐Ting; Rao, Jiahui; Feng, Chun

doi:10.1016/j.bbrc.2016.04.073

Cited by 33 publications

(15 citation statements)

References 34 publications

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“…miR-24-3p, predicted for three GPxs, plays a complex role in adipogenesis and mature adipocytes. This miRNA promotes adipocyte differentiation by direct targeting MAPK7 signaling [ 69 ]. On the other hand, Kang et al reported the inhibitory effect of miR-24 on adipocyte differentiation and maturity through targeting the fatty acid-binding protein 4 [ 148 ].…”

Section: Discussionmentioning

confidence: 99%

“…Many other miRNAs have been identified as possible GPx3 regulators reported in connection to oxidative stress and related pathologies. Examples include miR-34c-5p, reported as induced by hypoxia in mouse lungs [ 64 ] and significantly over-expressed in T2DM monocytes [ 65 ]; miR-379-5p, upregulated in mice fed with a high-fat diet [ 1 ]; miR-575, expressed in the amnion of obese pregnant woman only, whereas not detected in the amnion of controls [ 66 ]; miR-642a-3p, an adipocyte-specific miRNA upregulated in the fat depots of obese subjects [ 67 ]; an exosomal miR-4269 downregulated in obese donors [ 68 ]; miR-24-3p, which promotes adipocyte differentiation by directly targeting mitogen-activated protein kinase 7 (MAPK7) signaling [ 69 ] and with a high fat diet shows upregulated expression in mice livers, with the miR-24 inhibitor suggested as potential therapeutic agent for nonalcoholic fatty liver disease [ 70 ].…”

Section: Bioinformatic Analysis: Micrornas and Gpxsmentioning

confidence: 99%

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MicroRNAs as Potential Regulators of Glutathione Peroxidases Expression and Their Role in Obesity and Related Pathologies

Matoušková

Hanousková

Skálová

2018

IJMS

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Glutathione peroxidases (GPxs) belong to the eight-member family of phylogenetically related enzymes with different cellular localization, but distinct antioxidant function. Several GPxs are important selenoproteins. Dysregulated GPx expression is connected with severe pathologies, including obesity and diabetes. We performed a comprehensive bioinformatic analysis using the programs miRDB, miRanda, TargetScan, and Diana in the search for hypothetical microRNAs targeting 3′untranslated regions (3´UTR) of GPxs. We cross-referenced the literature for possible intersections between our results and available reports on identified microRNAs, with a special focus on the microRNAs related to oxidative stress, obesity, and related pathologies. We identified many microRNAs with an association with oxidative stress and obesity as putative regulators of GPxs. In particular, miR-185-5p was predicted by a larger number of programs to target six GPxs and thus could play the role as their master regulator. This microRNA was altered by selenium deficiency and can play a role as a feedback control of selenoproteins’ expression. Through the bioinformatics analysis we revealed the potential connection of microRNAs, GPxs, obesity, and other redox imbalance related diseases.

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Section: Discussionmentioning

confidence: 99%

Section: Bioinformatic Analysis: Micrornas and Gpxsmentioning

confidence: 99%

MicroRNAs as Potential Regulators of Glutathione Peroxidases Expression and Their Role in Obesity and Related Pathologies

Matoušková

Hanousková

Skálová

2018

IJMS

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“…Interestingly, some miRNAs have been verified to affect adipocyte biology, adipocyte differentiation, and adipose tissue function. For instance, miR-143, miR-17-92 cluster, miR-30c, and miR-24 promote adipocyte differentiation by negatively regulating extracellular signal-regulated kinase 5 ( ERK5 ) (Esau et al, 2004), tumor-suppressor Rb2/p130 (Wang et al, 2008), activin receptor-like kinase 2 ( ALK2 ) (Karbiener et al, 2011), and mitogen-activated protein kinase 7 ( MAPK7 ) (Jin et al, 2016), respectively. miR-27b, miR-200b, miR-26b, and miR-215 impair adipocyte differentiation by targeting PPARγ (Karbiener et al, 2009), Kruppel like factor 4 ( Klf4 ) (Shen et al, 2018), phosphatase, tensin homolog gene ( PTEN ) (Song et al, 2014), and fibronectin type III domain containing 3B ( FNDC3B ) (Peng et al, 2016), respectively.…”

Section: Introductionmentioning

confidence: 99%

miR-144-3p Promotes Adipogenesis Through Releasing C/EBPα From Klf3 and CtBP2

Shen

Wang

et al. 2018

Front. Genet.

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MicroRNAs (miRNAs), a class of small non-coding RNAs, have been proved as novel and potent regulators of adipogenesis. A previous study has found out that miR-144-3p was a biomarker of type 2 diabetes, but the role of miR-144-3p in regulating adipogenesis was still unclear. In the present study, the expression of miR-144-3p increased in obese mice and during the 3T3-L1 differentiation process. Overexpression of miR-144-3p suppressed the expression of cell cycle regulatory factors and inhibited pre-adipocytes proliferation. Besides, overexpression of miR-144-3p accelerated lipid accumulation in adipocytes and positively regulated adipogenesis, which was also accompanied by increasing the expression of genes related to fatty acid synthesis and decreasing the expression of genes involved in fatty acid oxidation. Furthermore, luciferase activity assays indicated that miR-144-3p directly targeted Klf3 and CtBP2. The process was also confirmed by the mRNA and protein expression of Klf3 and CtBP2, which were suppressed by miR-144-3p. Furthermore, miR-144-3p targeting Klf3/CtBP2 would induce C/EBPα activity by releasing corepressors (Klf3 and CtBP2) from its promoter region. Moreover, we also observed that miR-144-3p could promote adipogenesis in mice injected with miR-144-3p agomir through tail-vein injection. Taken together, these results support that miR-144-3p can facilitate adipogenesis both in vitro and in vivo, which implies that miR-144-3p could be a target for therapeutic intervention in obesity and metabolic syndrome in the future.

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“…In turn, the mirPath v.3 and TarBase v7.0 DIANA bioinformatic tools found that this microRNA modulates expression of the SCD gene, as well as fatty acid synthase ( FASN ), acetyl-CoA carboxylase α ( ACACA) , acetyl-coA acyltransferase 1 and 2 ( ACAA1 and ACAA2 ), and malonyl coA-acyl carrier protein transacylase ( MCAT ). miR-24-3p has been established to play a role in adipogenesis via activating adipocyte differentiation by targeting genes engaged in MAPK7-signaling pathways [ 44 , 45 ]. To date, only a few reports have been published concerning the possible involvement of miR-24-3p in lipid metabolism processes and obesity development.…”

Section: Discussionmentioning

confidence: 99%

Identification of Molecular Mechanisms Related to Pig Fatness at the Transcriptome and miRNAome Levels

Ropka‐Molik

Pawlina-Tyszko

Żukowski

et al. 2020

Genes

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Fat deposition and growth rate are closely related to pork quality and fattening efficiency. The next-generation sequencing (NGS) approach for transcriptome and miRNAome massive parallel sequencing of adipocyte tissue was applied to search for a molecular network related to fat deposition in pigs. Pigs were represented by three breeds (Large White, Pietrain, and Hampshire) that varied in fat content within each breed. The obtained results allowed for the detection of significant enrichment of Gene Ontology (GO) terms and pathways associated directly and indirectly with fat deposition via regulation of fatty acid metabolism, fat cell differentiation, inflammatory response, and extracellular matrix (ECM) organization and disassembly. Moreover, the results showed that adipocyte tissue content strongly affected the expression of leptin and other genes related to a response to excessive feed intake. The findings indicated that modification of genes and miRNAs involved in ECM rearrangements can be essential during fat tissue growth and development in pigs. The identified molecular network within genes and miRNAs that were deregulated depending on the subcutaneous fat level are proposed as candidate factors determining adipogenesis, fatness, and selected fattening characteristics in pigs.

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MicroRNA-24 promotes 3T3-L1 adipocyte differentiation by directly targeting the MAPK7 signaling

Cited by 33 publications

References 34 publications

MicroRNAs as Potential Regulators of Glutathione Peroxidases Expression and Their Role in Obesity and Related Pathologies

MicroRNAs as Potential Regulators of Glutathione Peroxidases Expression and Their Role in Obesity and Related Pathologies

miR-144-3p Promotes Adipogenesis Through Releasing C/EBPα From Klf3 and CtBP2

Identification of Molecular Mechanisms Related to Pig Fatness at the Transcriptome and miRNAome Levels

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