MicroRNA‑217 functions as a tumor suppressor in cervical cancer cells through targeting Rho‑associated protein kinase 1

Dong, Jing; Wang, Maoxiu; Ni, Donghua; Zhang, Lixin; Wang, Wen; Cui, Xiujuan; Fu, Shoukuan; Yao, Shujuan

doi:10.3892/ol.2018.9335

Cited by 12 publications

(20 citation statements)

References 41 publications

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“…Therefore, the ability of miR-217 to suppress cervical cancer may be modulated through ROCK1. This was further supported by suppressed luciferase activity of pmirGLO-ROCK1-3'UTR-1 and pmirGLO-ROCK1-3'UTR-2 following overexpression of miR-217 (123). Lastly, western blot analysis revealed that overexpression of miR-217 significantly lowered ROCK1 expression levels, whereas reduced levels of miR-217 expression resulted in increased ROCK1 expression levels (Fig.…”

Section: Cancer Type Tlrsmentioning

confidence: 60%

“…Expression of miR-217 in cervical cancer. Previous studies have revealed that miRNAs act as tumour suppressors in (123). Therefore, the ability of miR-217 to suppress cervical cancer may be modulated through ROCK1.…”

Section: Cancer Type Tlrsmentioning

confidence: 99%

“…7) (120). Therefore, miR-217 may target ROCK1 to inhibit cervical cancer proliferation (123). The level of miRNA transcription serves an important role in cervical cancer progression, which in turn highlights the importance of broadening our knowledge on potential miRNA target genes, as well as the potential mechanisms of migration and invasion for the identification of potential prognostic and diagnostic tools, and for the development of novel immunotherapeutic strategies for cervical cancer.…”

Section: Cancer Type Tlrsmentioning

confidence: 99%

“…Previous studies have revealed that miRNAs act as tumour suppressors in cervical cancer cells by regulating specific target genes. For example, Dong et al ( 123 ) compared Rho-associated coiled-coil containing protein kinase 1 (ROCK1) expression levels in cervical cancer and non-cancerous tissues and cell lines. Significantly lower expression levels of miR-217 were observed in metastatic and non-metastatic cervical cancer tissues and cell lines compared with those in non-cancerous tissues and cell lines ( 123 ).…”

Section: Factors That Contribute To the Development Of Cervical Cancementioning

confidence: 99%

“…For example, Dong et al ( 123 ) compared Rho-associated coiled-coil containing protein kinase 1 (ROCK1) expression levels in cervical cancer and non-cancerous tissues and cell lines. Significantly lower expression levels of miR-217 were observed in metastatic and non-metastatic cervical cancer tissues and cell lines compared with those in non-cancerous tissues and cell lines ( 123 ). Reduced invasive capabilities of SiHa and HeLa cell lines, as well as reduced colony formation, was demonstrated when miR-217 was highly expressed; in addition, the effect of high miR-217 expression on apoptosis in SiHa and HeLa cell lines was analysed by flow cytometry.…”

Section: Factors That Contribute To the Development Of Cervical Cancementioning

confidence: 99%

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Cervical cancer in low and middle‑income countries (Review)

et al. 2020

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Cervical cancer is a malignant tumour that occurs in the cervix and is classified into two histological types, adenocarcinoma and squamous cell carcinoma (SCC); SCC is more common and accounts for 70% of all cases. In 2018 there were ~569,000 new cases of cervical cancer diagnosed worldwide and ~311,000 deaths were attributed to cervical cancer. Of these, between 84 and 90% occurred in low-and middle-income countries (LMICs) such as South Africa, India, China and Brazil. The most common cause of cervical cancer is persistent infection caused by the sexually transmitted human papilloma virus. Other factors that contribute to the incidence of cervical cancer include geography, traditional practices and beliefs, the screening levels, socioeconomic status, healthcare access, public awareness, use of oral contraceptives, smoking and co-infection with HIV. An estimated 11 million women from LMICs will be diagnosed with cervical cancer in the next 10-20 years. The aim of this review was to explore various types of genetic and epigenetic factors that influence the development, progression or suppression of cervical cancer. Contents

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Section: Cancer Type Tlrsmentioning

confidence: 60%

Section: Cancer Type Tlrsmentioning

confidence: 99%

Section: Cancer Type Tlrsmentioning

confidence: 99%

Section: Factors That Contribute To the Development Of Cervical Cancementioning

confidence: 99%

Section: Factors That Contribute To the Development Of Cervical Cancementioning

confidence: 99%

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Cervical cancer in low and middle‑income countries (Review)

et al. 2020

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miR‐217 alleviates high‐glucose‐induced vascular smooth muscle cell dysfunction via regulating ROCK1

Zhou

Wang

2020

J Biochem & Molecular Tox

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MicroRNA‐217 (miR‐217) has been recently reported to be abnormally expressed during atherosclerosis. Nonetheless, it still remains unknown whether miR‐217 can regulate inflammation, proliferation, migration, and apoptosis of vascular smooth muscle cells (VSMCs) in high‐glucose condition. Sprague Dawley rats were used for establishing diabetic animal models. miR‐217 mimics and miR‐217 inhibitors were transfected into VSMCs. The miR‐217 and ROCK1 expressions were measured by quantitative reverse transcription‐polymerase chain reaction and Western blot. VSMCs’ proliferation, migration, cell cycle, and apoptosis were validated using the Cell Counting Kit‐8 assay, Transwell assay, and flow cytometry analysis, respectively. The binding sites between miR‐217 and the 3′‐untranslated region of ROCK1 were predicted via miRanda, PicTar, TargetScan, and microT databases, and the targeting relationship was confirmed by dual‐luciferase reporter experiments. miR‐217 was found to be upregulated in VSMCs treated by high glucose and aorta VSMCs of diabetic rats. Transfection of miR‐217 mimics significantly induced VSMCs cycle arrest, inhibition of proliferation, reduction of migration, and enhancement of apoptosis. The bioinformatics analysis and dual‐luciferase reporter experiments identified ROCK1 as a direct target of miR‐217. miR‐217 inhibits excessive proliferation and migration of VSMCs induced by high glucose by targeting ROCK1.

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hsa_circ_0119412 overexpression promotes cervical cancer progression by targeting miR‐217 to upregulate anterior gradient 2

Wang

Chen

et al. 2022

Clinical Laboratory Analysis

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Background: Mounting evidence summarizes that circRNA is closely implicated in the development of numerous cancers. Our study aimed to investigate the role of circ_0119412 whose function was not explored in cervical cancer.Methods: RT-qPCR analysis was utilized for the expression analysis of circ_0119412, miR-217, and anterior gradient 2 (AGR2). CCK-8 assay, transwell assay, and MTT assay were employed to assess cell proliferation, migration, and adhesion, respectively.Animal study was performed to check the role of circ_0119412 in vivo. Bioinformatics analysis was applied to predict the downstream targets of circ_0119412. RIP assay was utilized to examine miRNAs potentially bound by circ_0119412. The interplays between miR-217 and circ_0119412 or AGR2 were validated by dual-luciferase reporter assay.Results: circ_0119412 expression was highly enhanced in cervical tumor tissues and cancer cells. circ_0119412 overexpression aggravated cervical cancer cell proliferation, migration, and adhesion, and its overexpression was also conducive to tumor formation and growth in animal models. AGR2 was upregulated in cervical cancer by the public bioinformatics data. circ_0119412 bound to miR-217, and miR-217 bound to AGR 3'UTR. The promoting effects of circ_0119412 overexpression on cancer cell malignant phenotypes were reversed by miR-217 enrichment. In addition, increased expression of miR-217 suppressed AGR2 expression, thus weakening the functional effects of AGR2.Conclusion: circ_0119412 functioned as an oncogenic driver to promote the malignant development of cervical cancer by targeting the miR-217/AGR2 pathway.

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MicroRNA‑217 functions as a tumor suppressor in cervical cancer cells through targeting Rho‑associated protein kinase 1

Cited by 12 publications

References 41 publications

Cervical cancer in low and middle‑income countries (Review)

Cervical cancer in low and middle‑income countries (Review)

miR‐217 alleviates high‐glucose‐induced vascular smooth muscle cell dysfunction via regulating ROCK1

hsa_circ_0119412 overexpression promotes cervical cancer progression by targeting miR‐217 to upregulate anterior gradient 2

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