MicroRNA-214 protects L6 skeletal myoblasts against hydrogen peroxide-induced apoptosis

Chen, Wei; Zhang, Yanan; Jia, Qiongqiong; J, An; Shao, Suxia; Zhang, Lei; Gong, Miao; Yin, Qing; Huang, Xinli

doi:10.1080/10715762.2020.1730828

Cited by 7 publications

(3 citation statements)

References 47 publications

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“…Activated effector caspases complete apoptosis through the breakdown of proteins necessary for cell survival [28,31]. Indeed, we observed the release of cytochrome c from mitochondria to the cytoplasm due to disruption of mitochondrial membrane stability in H 2 O 2 -treated C2C12 cells, consistent with previous studies [35,36]. In addition, caspase-9 and caspase-3 were activated, which correlated with the degradation of PARP, a representative substrate protein cleaved by activated effector caspases [31,36].…”

Section: Discussionsupporting

confidence: 91%

Aqueous extracts of Corni Fructus protect C2C12 myoblasts from DNA damage and apoptosis caused by oxidative stress

2022

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Background Although the various pharmacological effects of Corni Fructus are highly correlated with its antioxidant activity, the blocking effect against oxidative stress in muscle cells is not clear. The purpose of this study was to investigate the effect of aqueous extracts of Corni Fructus (CFE) against oxidative stress caused by hydrogen peroxide (H 2 O 2 ) in murine skeletal C2C12 myoblasts.Methods and results MTT assay for cell viability, DCF-DA staining for reactive oxygen species (ROS) production, Comet assay for DNA damage, annexin V-FITC and PI double staining for apoptosis, JC-1 staining and caspase assay for monitor mitochondrial integrity, and western blotting for related protein levels were conducted in H 2 O 2 oxidative stressed C2C12 cells. Our results showed that CFE pretreatment signi cantly ameliorated the loss of cell viability and inhibited apoptosis in H 2 O 2 -treated C2C12 cells in a concentration-dependent manner. DNA damage induced by H 2 O 2 was also markedly attenuated in the presence of CFE, which was associated with suppression of ROS generation. In addition, H 2 O 2 reduced mitochondrial membrane potential and caused downregulation of Bcl-2 and upregulation of Bax expression, although these were abrogated by CFE pretreatment. Moreover, CFE blocked H 2 O 2 -induced cytosolic release of cytochrome c, activation of caspase-9 and caspase-3, and degradation of poly (ADPribose) polymerase.Conclusion Taken together, the present results demonstrate that CFE could protect C2C12 cells from H 2 O 2 -induced damage by eliminating ROS generation, thereby blocking mitochondria-mediated apoptosis pathway. These results indicate that CFE has therapeutic potential for the prevention and treatment of oxidative stress-mediated myoblast injury.

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Section: Discussionsupporting

confidence: 91%

Aqueous extracts of Corni Fructus protect C2C12 myoblasts from DNA damage and apoptosis caused by oxidative stress

2022

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“…[153] whereas in rat cardiomyocytes miR-181a which targets GPX1 is downregulated below 100µM H 2 O 2 and upregulated above this concentration [116]. Similar dose-dependent effects were also observed for miRNAs targeting transcripts of other redox regulating enzymes including NOX, NOS, CAT, GPX, GSR, PRDX, TXN and TXNRD [83,116,154,155] as summarized in Table 4. [141,147] ** miR-17 and miR-148a levels were decreased, and thus a pro-apoptotic effect is expected.…”

Section: Mir-17 Mir-148asupporting

confidence: 53%

“…For example, in ARPE-19 cells miR-23a which targets SOD2 and TXNRD1 is upregulated by H2O2 at concentrations up to 200 µM, but downregulated at higher concentrations [153] whereas in rat cardiomyocytes miR-181a which targets GPX1 is downregulated below 100µM H2O2 and upregulated above this concentration [116]. Similar dose-dependent effects were also observed for miRNAs targeting transcripts of other redox regulating enzymes including NOX, NOS, CAT, GPX, GSR, PRDX, TXN and TXNRD [83,116,154,155] as summarized in Table 4. Pro-apoptotic Myocardial cells of diabetic mice, hepatocytes [141,147] ** miR-17 and miR-148a levels were decreased, and thus a pro-apoptotic effect is expected.…”

Section: Changes Of Cellular H2o2 Levels Are Accompanied By Changes In Levels Of Mirnas and Their Targetsmentioning

confidence: 63%

Micro RNAs in Regulation of Cellular Redox Homeostasis

Ciesielska

Ślęzak-Prochazka

Bil

et al. 2021

IJMS

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In living cells Reactive Oxygen Species (ROS) participate in intra- and inter-cellular signaling and all cells contain specific systems that guard redox homeostasis. These systems contain both enzymes which may produce ROS such as NADPH-dependent and other oxidases or nitric oxide synthases, and ROS-neutralizing enzymes such as catalase, peroxiredoxins, thioredoxins, thioredoxin reductases, glutathione reductases, and many others. Most of the genes coding for these enzymes contain sequences targeted by micro RNAs (miRNAs), which are components of RNA-induced silencing complexes and play important roles in inhibiting translation of their targeted messenger RNAs (mRNAs). In this review we describe miRNAs that directly target and can influence enzymes responsible for scavenging of ROS and their possible role in cellular redox homeostasis. Regulation of antioxidant enzymes aims to adjust cells to survive in unstable oxidative environments; however, sometimes seemingly paradoxical phenomena appear where oxidative stress induces an increase in the levels of miRNAs which target genes which are supposed to neutralize ROS and therefore would be expected to decrease antioxidant levels. Here we show examples of such cellular behaviors and discuss the possible roles of miRNAs in redox regulatory circuits and further cell responses to stress.

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The Inhibitory Effect of Corni Fructus against Oxidative Stress-induced Cellular Damage in C2C12 Murine Myoblasts

Kim

Jeung²,

Park

et al. 2022

Biotechnol Bioproc E

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MicroRNA-214 protects L6 skeletal myoblasts against hydrogen peroxide-induced apoptosis

Cited by 7 publications

References 47 publications

Aqueous extracts of Corni Fructus protect C2C12 myoblasts from DNA damage and apoptosis caused by oxidative stress

Aqueous extracts of Corni Fructus protect C2C12 myoblasts from DNA damage and apoptosis caused by oxidative stress

Micro RNAs in Regulation of Cellular Redox Homeostasis

The Inhibitory Effect of Corni Fructus against Oxidative Stress-induced Cellular Damage in C2C12 Murine Myoblasts

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