volume 10, issue 6, P1474-1488 2018
DOI: 10.18632/aging.101484
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Ronghan Liu, Wenzhao Wang, Shuya Wang, Wei Xie, Hongfei Li, Bin Ning

Abstract: Astrogliosis following spinal cord injury (SCI) was considered as a negative factor for neural regeneration. We found that miR-21 was significantly upregulated after SCI. So, we aim to determine whether miR-21 acts in a positive manner post SCI. In vitro, we measured the proliferation, apoptosis and cytokine secretion of primary cultured astrocytes after modulating the expression of miR-21 by western blot, RT-PCR and immunofluorescence. In vivo, we performed a modified Allen’s weight drop model. Manipulation o…

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