MicroRNA‐148b suppresses cell growth by targeting cholecystokinin‐2 receptor in colorectal cancer

Song, Yongxi; Xu, Yingying; Wang, Zhenning; Chen, Yue; Yue, Zhenyu; Gao, Peng; Xing, Chengzhong; Xu, Hui

doi:10.1002/ijc.26485

Cited by 83 publications

(73 citation statements)

References 42 publications

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“…The expression of miR-203 is associated with tumor size, advanced Borrmann type and pT category of the GC (22). In addition, miR-148b is able to suppress cell growth in GC and colorectal cancer by targeting cholecystokinin B receptor (8,23), indicating that a number of miRNAs have the same function in different types of cancer.…”

Section: Discussionmentioning

confidence: 99%

“…They are endogenous non-coding small molecule RNAs, between 19 and 24 ribonucleotides in length, which post-transcriptionally regulate gene expression in plants and animals (2,4). miRNAs have important roles in regulating a number of cell processes, including cell differentiation, apoptosis and cell cycle progression (5)(6)(7)(8)(9). miRNAs serve as biomarkers in various types of cancer, including GC and prostate cancer (10,11).…”

Section: Introductionmentioning

confidence: 99%

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MicroRNA-1258: An invasion and metastasis regulator that targets heparanase in gastric cancer

et al. 2017

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Abstract. Numerous microRNAs (miRNAs/miRs) are involved in suppressing or promoting the formation of cancer. However, to the best of our knowledge, the role of miRNA-1258 in gastric cancer (GC) has not previously been investigated. Our previous study demonstrated an increased expression of heparanase (HPSE) in GC tissues and HPSE-facilitated invasion and metastasis of GC cells. Consequently, in the present study, the function of miR-1258 in the invasion and metastasis of GC cells was investigated to determine whether miR-1258 is associated with GC through HPSE. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to determine miR-1258 expression in GC cell lines and tissues. A Transwell cell invasion assay and an MTT proliferation assay were used to investigate the effects of miR-1258 on the invasion and proliferation of one of the cell lines, SGC-7901 cells, in vitro. The effect of miR-1258 on SGC-7901 cell metastasis was investigated using an in vivo tumor metastasis formation assay. Western blot analysis and RT-qPCR were used to investigate whether HPSE was a target of miR-1258 in GC. The expression of miR-1258 was significantly downregulated in 3 GC cell lines and 116 GC tissues compared with controls (all P<0.001). An association was identified between decreased miR-1258 expression level and increased age (P=0.042), advanced pathological tumor stage (P=0.027) and positive lymphatic vessel invasion (P=0.044) in patients with GC. Furthermore, the upregulation of miR-1258 expression suppressed SGC-7901 cell invasion in vitro (P<0.001) and inhibited SGC-7901 cell metastasis in vivo (P=0.016). The western blot analysis and RT-qPCR results indicated that miR-1258 downregulated the expression of HPSE at the translational level. The results of the present study indicate that miR-1258 acts as a tumor suppressor to inhibit invasion and metastasis by targeting HPSE. Therefore, miR-1258 may serve as a novel biomarker and therapeutic target in the treatment of GC.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

MicroRNA-1258: An invasion and metastasis regulator that targets heparanase in gastric cancer

et al. 2017

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“…In plasma, Cuk et al noted that miR- 148b was significantly upregulated in breast cancer patients [37]. Especially, the studies of Song et al showed that miR-148b was downregulated in gastric cancer [38], colorectal cancer [19] and suppressed cell growth by targeting cholecystokinin-2 receptor(CCK2R). Differently, down-expression of miR-152 could lead to the impaired anti-tumor effect of killer cell in hepatocellular carcinoma via upregulation of HLA-G [39].…”

Section: Discussionmentioning

confidence: 99%

MiR-148b, MiR-152/ALCAM Axis Regulates the Proliferation and Invasion of Pituitary Adenomas Cells

Huang

et al. 2017

Cell Physiol Biochem

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Background/Aims: Aberrant expression of miRNA has been found in many tumor tissues to regulate the tumorigenesis by binding to the 3`- untranslated region (3`-UTR) of the target genes. The aim of this study is to investigate the role of miR-148b, miR-152/ALCAM axis in human pituitary adenomas (PAs). Methods: First, we detected the expression level of miR-148b-3p and miR-152 in human PAs samples by using qRT-PCR. Then we studied the role of miR-148b-3p, miR-152 on human PAs cell proliferation, invasion and apoptosis by using MTS assay, Transwell invasion assay and Annexin V/PI Staining Test. To study the relationship between miR-148b-3p, miR-152 and activated leukocyte antigen molecule (ALCAM), we overexpressed miR-148-3p or miR-152 by transfecting specific mimics. Lucifearase reporter assay was then performed to confirm the target. Next, we studied the biological functions of ALCAM in human PAs cells. Finally, the role of miR-148b-3p, miR-152/ALCAM axis in PAs cells was studied. Results: The expression level of miR-148-3p and miR-152 in invasive PAs samples was lower than those in noninvasive samples. Overexpression of miR-148b-3p, miR-152 could repress proliferation and invasion, and promote apoptosis. Moreover, miR-148b-3p and miR-152 could repress activated leukocyte antigen molecule (ALCAM) expression. Knockdown of ALCAM could repress proliferation and invasion and promote apoptosis. By contrary, overexpression of ALCAM promoted proliferation and invasion. Further, the rescue experiments indicated that overexpression of ALCAM significantly restored the proliferation, apoptosis, and invasion influenced by miR-148b-3p and miR-152. Conclusions: Our study suggests that miR-148b-3p, miR-152 may serve as suppressors in PAs through downregulating ALCAM expression. miR-148b, miR-152/ ALCAM axis may be a new therapeutic target in the future.

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“…Microarray studies have identified a number of miRNAs that are up-or downregulated in pancreatic cancer. Recently, miR148b has been shown to be deregulated in some other types of cancers, such as overexpressed in ovarian cancer (12) and lung cancer (13), whereas downregulated in pancreatic cancer (14,15), gastric cancer (16), and colorectal cancer (17). However, the exact role of miR-148b in carcinogenesis of pancreatic cancer has not been revealed yet.…”

Section: Introductionmentioning

confidence: 99%

miR-148b Functions as a Tumor Suppressor in Pancreatic Cancer by Targeting AMPKα1

Zhao

Zhang

Liu

et al. 2013

Molecular Cancer Therapeutics

106

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miRNAs are small noncoding RNAs that participate in a variety of biologic processes, and dysregulation of miRNA is always associated with cancer development and progression. Aberrant expression of miR-148b has been found in some types of cancer, but its expression and potential biologic role in pancreatic cancer are still largely unknown. In this study, our data showed that miR-148b was significantly downregulated in 48 pairs of human pancreatic cancer tissues and five cell lines. Furthermore, the deregulated miR-148b was correlated with increased tumor size, late tumor-node-metastasis stage, lymphatic invasion, distant metastasis, and worse prognosis in pancreatic cancer. Functional studies indicated overexpression of miR-148b dramatically suppressed the growth of cancer cells, attributable to induction of apoptosis and cell-cycle arrest at S-phase. Meanwhile, miR-148b remarkably inhibited invasion and enhanced chemosensitivity of pancreatic cancer cells. Moreover, ectopic expression of miR-148b was able to inhibit tumorigenicity in nude mice. Further studies revealed that AMPKa1 might be the direct target gene of miR-148b, and overexpressed AMPKa1 inversely correlated with miR-148b in pancreatic cancer. Silencing of AMPKa1 with RNA interference inhibited the growth of pancreatic cancer cells in vitro and in vivo and also induced apoptosis, cell-cycle arrest, and inhibited invasion of cancer cells, which is consistent with the effects of miR-148b overexpression. In conclusion, miR-148b can inhibit cell proliferation, invasion, and enhance chemosensitivity of pancreatic cancer by targeting AMPKa1. Our present results implicate the potential effects of miR-148b on prognosis and treatment of pancreatic cancer.

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MicroRNA‐148b suppresses cell growth by targeting cholecystokinin‐2 receptor in colorectal cancer

Cited by 83 publications

References 42 publications

MicroRNA-1258: An invasion and metastasis regulator that targets heparanase in gastric cancer

MicroRNA-1258: An invasion and metastasis regulator that targets heparanase in gastric cancer

MiR-148b, MiR-152/ALCAM Axis Regulates the Proliferation and Invasion of Pituitary Adenomas Cells

miR-148b Functions as a Tumor Suppressor in Pancreatic Cancer by Targeting AMPKα1

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