MicroRNA-148b is frequently down-regulated in gastric cancer and acts as a tumor suppressor by inhibiting cell proliferation

Song, Yongxi; Yue, Zhenyu; Wang, Zhenning; Xu, Yingying; Luo, Yang; Xu, Hui; Zhang, Xue; Jiang, Li; Xing, Chengzhong; Zhang, Yong

doi:10.1186/1476-4598-10-1

Cited by 269 publications

(251 citation statements)

References 48 publications

(52 reference statements)

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“…As shown on the miRBase website, miR-152 and miR-148a have the same seed region, which is 2nt-8nt of miRNA, and is considered the most important recognition site (37). Other studies have shown that miR-148a, miR-148b, and miR-152, all from the same family, have the same seed sequences as well, and also regulate the same target, namely CCKBR, in gastric cancer (38). Stumpel et al also demonstrated that miR-148 and miR-152 have the same seed sequence, and target DNMT1 together (39).…”

Section: Mir-152 and Mir-148a Have No Effect On Invasion Of Skov3 Cellsmentioning

confidence: 99%

Altered expression of miR-152 and miR-148a in ovarian cancer is related to cell proliferation

Zhou

Zhao²,

Wang

et al. 2011

Oncol Rep

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Abstract. microRNAs (miRs) are endogenous small noncoding RNAs that are aberrantly expressed in various carcinomas. miR-152 and miR-148a have not been comprehensively investigated in ovarian cancer. Thus, the aim of this study was to identify the role of miR-152 and miR-148a in epithelial ovarian cancer. Total RNA was extracted from tissues of 78 patients with epithelial ovarian cancer, 17 normal ovarian epithelium tissues and two ovarian cancer cell lines. Using quantitative real-time PCR (qRT-PCR) followed by the 2 -ΔΔCT method for calculating the results, we found that the expression levels of miR-152 were significantly decreased in ovarian cancer tissues compared to normal ovarian epithelium tissues (p<0.05). However, although the expression of miR148a was also decreased in 65% of patients, no statistically significant difference in expression was found. A strong correlation was found between the expression of miR-152 and miR-148a (p<0.001, Pearson's correlation). The relationship between miR-152 or miR-148a expression levels in ovarian cancer and clinicopathological features, response to therapy and short-term survival was analyzed and the results showed that no correlation existed. In addition, we found that both miR-152 and miR-148a were down-regulated in ovarian cancer cell lines. After miR-152 or miR-148a mimics were transfected into ovarian cancer cell lines, the MTT cell proliferation assay showed that cell proliferation was significantly inhibited. Taken together, miR-152 and miR-148a may be involved in the carcinogenesis of ovarian cancer through deregulation of cell proliferation. They may be novel biomarkers for early detection or therapeutic targets of ovarian cancer. IntroductionOvarian cancer is one of the most common causes of death from gynecological malignancies. In addition, with an incidence of ~15,000 deaths annually, it is the fifth leading cause of cancer-related deaths among women in the United States (1). More than 90% of ovarian cancers are epithelial ovarian cancers (EOCs), which are derived from the ovarian surface epithelium (2). Due to a lack of effective biomarkers, ineffective tools for ovarian cancer screening, and non-specific symptoms in its early stages, more than two-thirds of patients with ovarian cancer are not diagnosed until the disease is in an advanced stage (3). Despite advances in early detection and the current standard treatment for advanced ovarian cancer, the 5-year survival rate is only 20-25% (4,5), which makes the development of alternative approaches urgent. Understanding the molecular alterations of ovarian cancer will help identify novel diagnostic markers or therapeutic targets, thereby improving the survival rates in this population of cancer patients.microRNAs (miRNAs or miRs) are a class of highly conserved, non-coding RNAs, approximately 19-25 nucleotides in length. They function as post-transcriptional regulators by binding to the 3'UTR regions of protein-encoding genes, which results in translational repression and gene silencing (6,7). Almost 30% ...

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Section: Mir-152 and Mir-148a Have No Effect On Invasion Of Skov3 Cellsmentioning

confidence: 99%

Altered expression of miR-152 and miR-148a in ovarian cancer is related to cell proliferation

Zhou

Zhao²,

Wang

et al. 2011

Oncol Rep

Self Cite

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“…The expression of let-7f has also been proven to be decreased in metastatic gastric cancer tissues, while let-7f overexpression in gastric cancer was able to inhibit the invasion and migration of gastric cancer cells (14). Another study demonstrated the significant downregulation of miR-148b in 106 gastric cancer tissues and the consequent suppression of gastric cancer cell growth, suggesting that miR-148b could become a potential biomarker and therapeutic target against gastric cancer (15). (16)(17)(18)(19).…”

Section: Discussionmentioning

confidence: 99%

Expression and clinical significance of the microRNA-200 family in gastric cancer

Chang

Huo

et al. 2015

Oncology Letters

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Abstract. Gastric cancer is one of the most common malignant tumors and one of the leading causes of cancer-related mortality. Recent studies have revealed that there is a difference in microRNA (miR/miRNA) profiles between cancerous and normal tissues. To find a potentially useful prognostic predictor and a promising therapeutic tool for gastric cancer, the present study investigated the expression and clinical significance of the miR-200 family in gastric cancer. The miR-200 family has five members: hsa-miR-200a, hsa-miR-200b, hsa-miR-200c, hsa-miR-141 and hsa-miR-429. In 46 clinical samples of gastric cancer and paired non-cancerous tissues, the present study observed that the expression levels of the miR-200 family in the cancer tissues were significantly lower than those in the non-cancerous tissues (P<0.001). Lower levels of the five family members were associated with histological grade and the presence of an intravascular cancer embolus (P<0.05). The results revealed that the miR-200 family is downregulated in gastric cancer, and that there are significant differences in the expression of the miR-200 family between normal and cancer tissues. The miR-200 family may therefore become a potentially useful prognostic predictor of the aggressiveness of gastric cancer and a possible therapeutic tool in affected patients.

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“…Recent studies have indicated that several miRNAs, including miR-34b/c, miR-129, miR-137, miR-181C, miR-199a, miR-212, miR-512 and miR-516, were dysregulated and showed aberrant methylation patterns in gastric cancer cells. 6,9,[14][15][16][17][18][19][20][21] The three pri-miR-9 transcripts are all located within non-protein coding transcripts (Fig. 2A).…”

Section: Cell Lines and 5-aza-dc Treatmentmentioning

confidence: 99%

Aberrant hypermethylation ofmiR-9genes in gastric cancer

Tsai

Liao²,

Wu³

et al. 2011

Epigenetics

105

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MicroRNA-148b is frequently down-regulated in gastric cancer and acts as a tumor suppressor by inhibiting cell proliferation

Cited by 269 publications

References 48 publications

Altered expression of miR-152 and miR-148a in ovarian cancer is related to cell proliferation

Altered expression of miR-152 and miR-148a in ovarian cancer is related to cell proliferation

Expression and clinical significance of the microRNA-200 family in gastric cancer

Aberrant hypermethylation ofmiR-9genes in gastric cancer

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