Micropropagation ofDalbergia sissoo from nodal explants of mature trees

Abstract

A method for micropropagation of Dalbergia sissoo has been developed. Single node segments obtained from coppice shoots of a mature tree (20 -25 year old) produced 3 -4 shoots per explant on Murashige and Skoog (MS) medium containing 4.4 x 10 -6 M benzylaminopurine (BAP) and 4.4 x 10 -7 M of 13-naphthoxy acetic acid (NOA) (shoot multiplication medium) within 4 weeks. The in vitro regenerated shoots were 3 -4 cm in length and provided 2 to 3 culturable nodal segments which on shoot multiplication medium again produced 3 -4 shoots. Following this procedure 18 -24 shoots were produced from single nodal segment within 60 d. 80 % of the shoots directly produced five roots when they were firstly treated with MS medium supplemented with 10 -5 M indole-3-butyric acid (IBA) and subsequently transferred to half strength liquid MS medium containing 1% activated charcoal followed by half strength liquid MS free hormones, vitamins and activated charcoal. The in vitro raised plants were hardened for survival after transplantation to soil by exposing them to various humidity conditions, gradually from higher to low, with nearly I00 % transplant success.

Effect of exogenous phytohormones and sucrose on micropropagation and microtuberisation of Manihot esculenta Crantz var. TMS 96/0023

“…The nodal cuttings when cultured on MS supplemented with BAP or NAA budded after 11 days with higher percentages of 98% (0.07 mg l -1 BAP) and 73% (0.05 mg l -1 NAA). Comparable results have been showed in many herbaceous and ligneous species (Blagwat et al, 1996;Gulati and Jaiwal, 1996;Fotso, 2005). But in general, the rate of budding varies not only according to the species but also according to the nature and the concentration of phytohormone used.…”

Rapid multiplication of Dalbergia sissoo Roxb.: a timber yielding tree legume through axillary shoot proliferation and ex vitro rooting

“…1d; Table 3). The rate of shoot amplification achieved for D. sissoo in the liquid medium was higher than reported earlier (Datta and Datta 1983;Gulati and Jaiwal 1996;Joshi et al 2003;Thirunavoukkarasu et al 2010). Liquid culture offers many potential advantages over solid cultures like faster growth rates, rapid uptake of nutrients by tissues, and dilution of exuded growth inhibitors, i.e., phenolics released by explants, (Kim et al 2003;Gupta and Timmis 2005;Preece 2010).…”

“…Till date, in vitro plant regeneration of D. sissoo from explants of mature tree has also been reported, but these explants produced callus (Datta and Datta 1983) and less number of shoots (Joshi et al 2003;Thirunavoukkarasu et al 2010). Datta et al (1982) and Gulati and Jaiwal (1996) rooted the microshoots of D. sissoo under in vitro condition by two-step method. Generally, woody plant species are recalcitrant to adventitious regeneration during their maturation stage as the vigor for shoot production and competence for rooting declines (Singh et al 2002;Bonga et al 2010).…”

In vitro plant regeneration system for Cassia siamea Lam., a leguminous tree of economic importance

“…Micropropagation techniques can be used as an alternative tool for rapid establishment and conservation of control pollinated seeds. Although, the regeneration of forest trees in general and legumes in particular have been a difficult task (Lakshmisita et al 1992), the strategies to regenerate legumes by exploiting tissue culture techniques have been evolved steadily during the past few years (Rhagavaswamy et al 1992;Gulati and Jaiswal 1996;Shahzad et al 2006;Husain et al 2008). Thus, to ensure an availability of large number of planting propagule for afforestation programmes, biomass production and germplasm conservation an alternative and feasible method (micropropagation) is of great value.…”