Micropropagation of Some Watermelon Cultivars by Using Tissue Culture Technique

Amer, Mona G.; Arisha, H.; Ahmed, Aisha; Nawar, Dalia A.S.

doi:10.21608/zjar.2020.95903

Cited by 2 publications

(2 citation statements)

References 12 publications

(10 reference statements)

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“…In comparison to a semisolid MS medium containing 5 mg/l BAP, the same medium supplemented with different concentrations of moringa leaf extract decreased the number of shoots/explant. The retardation effect of moringa leaf explants was detected by Amer et al [67]. Antioxidants such as ascorbic acid were detected at high concentrations in moringa chloroplasts and other cellular components [68].…”

Section: Discussionmentioning

confidence: 92%

Alleviation of Tissue Browning During Clonal Propagation of Banana cv. Grand Naine

Hassanein,

Salem,

El-Deep

et al. 2023

Sohag Journal of Sciences

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Banana micropropagation is commercially established by shoot tips obtained from intact plants on a shoot multiplication medium under optimal culture conditions. However, this is somewhat associated with tissue browning, which may be associated with reduced efficiency of the shoot multiplication process. It was found that changes in culture conditions such as increase or decrease of incubation temperature around 27 ºC, change in the texture of the media from the solid or liquid to semisolid state, addition of ascorbic acid, and substitution of optimal concentration of benzyl amino purine (5 mg/l BAP) with 2-isopentyl adenine (4 mg/l 2ip), could reduce tissue browning but decreased shoot multiplication and growth. The present results indicated that the chemical composition of the MS medium containing 5 mg/l BAP was ideal for banana shoot multiplication. Adding some natural ingredients rich in many important components (yeast extract, coconut endosperm milk, or moringa leaf extract) was unsuitable for in vitro multiplication of bananas. The observed exception was that the growth parameters of shoots formed on MS medium of 4 mg/l 2ip were better than those formed on MS of 5 mg/l BAP. In addition, shoot multiplication on 2-isopentenyl adenine (2ip) containing media showed root formation, adaptation, and transfer of plants to soil.

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Section: Discussionmentioning

confidence: 92%

Alleviation of Tissue Browning During Clonal Propagation of Banana cv. Grand Naine

Hassanein,

Salem,

El-Deep

et al. 2023

Sohag Journal of Sciences

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“…Hasil serupa terjadi pada penelitian Erawati et al (2021), yang menunjukkan bahwa perlakuan BAP menghasilkan bobot basah kultur vanili (Vanilla planifolia) yang lebih berat dibandingkan dengan perlakuan Kinetin. Hal yang sama terjadi pada penelitian Amer et al (2013) yang menunjukkan bahwa perlakuan BAP menghasilkan bobot basah kultur yang lebih berat dibandingkan perlakuan Kinetin dalam konsentrasi yang sama pada tanaman stroberi. KESIMPULAN Berdasarkan hasil penelitian dapat disimpulkan bahwa penggunaan sitokinin jenis BAP lebih disarankan daripada penggunaan sitokinin jenis Kinetin untuk mendapatkan jumlah tunas tertinggi, jumlah daun tertinggi, dan bobot basah kultur tertinggi pada stroberi kultivar Sweet Charlie.…”

Section: Bobot Basahunclassified

Perbandingan Efek Bap Dan Kinetin Terhadap Laju Multiplikasi Stroberi Kultivar Sweet Charlie

Andriani,

Kadapi,

Suminar

et al. 2023

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Seed propagation of strawberry by in vitro culture is needed to obtain high quality seeds of strawberry in large quantities. In in vitro culture, a large number of shoots is expected can be produced at the multiplication stage. Cytokinins type of PGR can be added to culture media to produce a large number of shoot. The focus of this research was to compare the effect of BAP and Kinetin affected Sweet Charlie strawberry multiplication. The experiment was located at the Seed Technology Tissue Culture Laboratory, Faculty of Agriculture, Padjadjaran University. The experimental design was a Completely Randomized Design (CRD) with two treatments of different types of cytokinin, namely Benzyl Amino Purine (BAP) and Kinetin at 1 mg L-1 on Murashige and Skoog (MS) basic media, each treatment was repeated four times. This study showed that BAP had a better effect than Kinetin on the number of shoots, number of leaves, and wet weight of strawberry Sweet Charlie cultivar. The addition of BAP was recommended over Kinetin to obtain the highest number of shoots, the highest number of leaves, and the highest wet weight of culture on strawberry Sweet Charlie cultivar.

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Micropropagation of Some Watermelon Cultivars by Using Tissue Culture Technique

Cited by 2 publications

References 12 publications

Alleviation of Tissue Browning During Clonal Propagation of Banana cv. Grand Naine

Alleviation of Tissue Browning During Clonal Propagation of Banana cv. Grand Naine

Perbandingan Efek Bap Dan Kinetin Terhadap Laju Multiplikasi Stroberi Kultivar Sweet Charlie

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