Micropropagation of Caralluma adscendens var. fimbriata-An Indigenous Medicinal Plant of India

Abstract

Present study describes micro-propagation of traditionally important medicinal plant in northern interior part of Karnataka, Caralluma adscendens var. fimbriata, for its conservation. Results showed that the survival ability of regenerated plants was 84 per cent. Proliferation of C. adscendens var. fimbriata shoots was achieved on medium supplemented with various concentrations of different hormones. The effective combination of 6-Benzylaminopurine (BA) 2.0 mg/l along with 0.5 mg/l kinetin (Kn) and 0.2 mg/l Indole-3-acetic acid (IBA) produced highest number of shoot sprouting frequency (91%) with a mean of 2.60 ± 0.17 shoot number per explants and a mean shoot length of 3.88 ± 0.20 cm from the mature nodal explants. A good frequency of callus was induced in 1.5 to 2.0 mg/l of 1-Naphthaleneacetic acid (NAA) and in 2.0 mg/l of 2,4-dichlorophenoxy acetic acid (2, 4-D) were transferred to root induction media. The NAA showed positive response of rooting when compared with IAA and IBA in the present study. The best root and shoot sprouted plants were successfully acclimatized in the greenhouse and transferred to open field conditions. This micropropagation protocol can be successfully used for large-scale multiplication and conservation of germplasm of this threatened plant.

One Pot Facile Bio-Synthesis of Anti-angiogenic Iron Oxide Nanoparticles Using the Bio-Reductant Sida spinosa

“…From the DLS distribution graph the size of the iron oxide nanoparticles is measured with the diameter of 91.3 nm. The size factor is very important in the nano field, which makes the nanoparticles to possess efficient properties and applications [35]. The nanoparticles dispersed in water with the dielectric constant (78.3), refractive index (1.33) and viscosity (0.88 cP) at 25°C.…”

Caesalpinia bonduc seeds assisted biosynthesis of Fe/ZnO photo-catalyst for degradation of methyl orange dye in aqueous medium

“…These phytochemicals possess antioxidant, anti-in ammatory, and antimicrobial properties [38,39]. These phytochemicals have shown to be excellent reducing and capping agents for synthesizing various metal nanoparticles [40]. In one of our recent studies, we successfully synthesized Ag/Bi/Sno 2 nanocomposite material using seed extracts of C. bonduc [41].…”