Micropropagation and experimental field cultivation of Pulsatilla turczaninovii Kryl. et Serg. (Ranunculaceae)

Hanus-Fajerska, Ewa; Kocot, Dawid; Wiszniewska, Alina; Koźmińska, Aleksandra; Muszyńska, Ewa

doi:10.1007/s11240-021-02140-0

Cited by 3 publications

(2 citation statements)

References 53 publications

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“…Several Pulsatilla species, e.g. P. grandis , P. koreana 47 , P. patens 12 , 47 , P. pratensis 48 – 50 , P. turczaninovii 51 , P. vernalis and P. vulgaris 33 , were multiplied by in vitro organogenesis and/or somatic embryogenesis, acclimatized to ex vitro and to semi-natural conditions. However, in none of these experiments, regenerated plants were re-introduced to the natural (extinct) populations, and their genetic uniformity was not confirmed with the initial material.…”

Section: Discussionmentioning

confidence: 99%

Re-introduction of an extinct population of Pulsatilla patens using different propagation techniques

Żabicka

Żabicki

Słomka

et al. 2022

Sci Rep

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The study focuses on the propagation of a rare and endangered plant species (Pulsatilla patens) to re-introduce an extinct population from calamine area in Southern Poland. The plants were propagated from seeds, rhizome cuttings, or regenerated in vitro from shoot tips, hypocotyls with roots or cotyledons of seedlings on Murashige & Skoog (MS) medium supplemented with 0.25 or 0.50 mg L−1 BAP (Benzylaminopurine) via direct and indirect organogenesis or somatic embryogenesis (SE). The most efficient micropropagation method was with shoot tips as an explant on MS + 0.25 mg L−1 BAP where 97% of the explants produced multiple shoots, mass SE was observed after transfer on ½ MS with 2% saccharose; 267 (35%) shoots rooted on ½ MS + 2% saccharose were acclimatized to ex vitro conditions. Flow cytometry revealed genome size stability of propagated plantlets. Low genetic differentiation between micropropagated plantlets and initial material was indicated by ISSR (Inter Simple Sequence Repeat) markers. Totally, 132 vigorous plantlets obtained on various pathways were introduced to the field plots in 2020; 30.33% survived the winter, and several reached the generative stage and flowered in the spring 2021. In next season (March/April 2022) the number of introduced plants decreased to 25% while the number of flowering and fruiting shoots in different clumps increased in some plots. This is the first report of successful re-introduction of the endangered P. patens based on micropropagation, rhizome cuttings, and seed germination.

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Section: Discussionmentioning

confidence: 99%

Re-introduction of an extinct population of Pulsatilla patens using different propagation techniques

Żabicka

Żabicki

Słomka

et al. 2022

Sci Rep

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“…Recently, it was reported that using seeds for plant propagation may increase genetic variability and maximize the repopulation of sexually originated plants such as Pulsatilla turczaninovii , Viola cornuta , Echinacea angustifolia and others [ 17 , 18 , 19 , 20 , 21 ]. However, there is some dispute about whether Agave species produce viable seeds for propagation [ 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of Seed-Derived Clonal Lines of the Endangered Agave marmorata Roezl and Their Compatibility with Endophytes

Martínez-Rodríguez

Beltran-Garcia

Salas

et al. 2022

Biology

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A. marmorata is the raw material used for tepextate mescal production but is classified as an endangered species. In the present study, we obtain and multiply clonal lines of Agave marmorata Roezl by selecting seedlings derived from seeds. Ten seedlings from two lots of 400 germinated seeds were selected for axillary bud proliferation induced by BAP 5 mg/L in vitamin-free Murashige and Skoog’s medium. Differences in shoot numbers, heights and senescent tissue formation were observed. Notably, the AM32 line formed 84 shoots and presented low senescent tissue after 60 d of culture. We also selected the AM31 and AM33 clonal lines. Four-month shoots were extracted with 80% methanol in water to determine the total content of saponins, flavonoids, and phenolic acids and compare the three clonal lines. Some bioactive molecules were identified using HPLC techniques and MALDI-TOF mass spectrometry none showed significant differences in content. Additionally, plants derived from the clonal lines were inoculated with four endophytic bacteria. Among these, Achromobacter xylosoxidans supported plant growth of AM32. A notable effect of plant death was observed after inoculation with Enterobacter cloacae, an endophyte of A. tequilana. Additionally, Pseudomonas aeruginosa, an endophyte from A. marmorata, reduced biomass. Our results demonstrate the incompatibility of A. marmorata to E. cloacae and specialization between the host plant and its endophytes. The compatibility of the plant-endophyte could be exploited to boost the establishment and stability of mutualisms to benefit plant development, stress tolerance and pathogen resistance. The differences in multiplication capacity, stable tissue formation, and endophyte biotization responses may indicate genetic variability. Clonal selection and micropropagation from seed-derived plants could contribute to conserving the endangered A. marmorata plant for reforestation in their natural habitats, thus, assuring mass propagation for sustainable industrial production of mescal, bioactive compounds, and prebiotics.

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In vitro shoot regeneration from organogenic callus culture and rooting of Carpathian endemic Aconitum bucovinense Zapał.

Kocot

Nowak

Sitek

et al. 2022

Plant Cell Tiss Organ Cult

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Aconitum bucovinense Zapał. is the European species of monkshood, endemic to the Eastern and Southern Carpathians. A protocol has been developed for the in vitro regeneration of adventitious shoots by indirect organogenesis from leaf explants. An initiation of cultures carried out on a medium with picloram and kinetin allowed obtaining a callus. More than a 200% FM increase of the callus and at the same time differentiation of adventitious buds were obtained on IBA and BAP supplemented media. Excised buds were used to establish shoot cultures and multiplied after a transfer to nutrient media with an addition of BAP with IBA, IAA or NAA. Almost 70% of rooted shoots were obtained on a 1.5 mg L − 1 IBA and 1.0 mg L − 1 BAP supplemented medium with simultaneous e cient multiplication. An analysis of peroxidase activity revealed its gradual increase in shoots until the appearance of roots. For the rst time, an e cient way to regenerate, multiply and root A. bucovinense shoots has been developed and can be used for ex situ conservation of this species. Key MassageThe system of plant regeneration of Aconitum bucovinense from callus culture was elaborated. The analysis of the peroxidase level showed that its content increases until the rst roots appear.

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Micropropagation and experimental field cultivation of Pulsatilla turczaninovii Kryl. et Serg. (Ranunculaceae)

Cited by 3 publications

References 53 publications

Re-introduction of an extinct population of Pulsatilla patens using different propagation techniques

Re-introduction of an extinct population of Pulsatilla patens using different propagation techniques

Micropropagation of Seed-Derived Clonal Lines of the Endangered Agave marmorata Roezl and Their Compatibility with Endophytes

In vitro shoot regeneration from organogenic callus culture and rooting of Carpathian endemic Aconitum bucovinense Zapał.

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