Microplate chemiluminescent assay for HBV DNA detection using 3-(10′-phenothiazinyl)propionic acid/N-morpholinopyridine pair as enhancer of HRP-catalyzed chemiluminescence

Bodulev, Oleg L.; Gribas, Anastasia V.; Sakharov, Ivan Yu.

doi:10.1016/j.ab.2017.11.026

Cited by 17 publications

(7 citation statements)

References 45 publications

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“…Bodulev et al utilized HRP-labeled oligonucleotide detection probes to specifically hybridize to hepatitis B viral DNA that was captured onto microtiter plates. 70 By exploiting the HRP-mediated oxidation of 3-(10′-phenothiazinyl)propionic acid/ N -morpholinopyridine, the group was able to detect the target DNA down to 3 pM using a chemiluminescent signal. A more recent and promising application of peroxidases is in electrochemical signal generation.…”

Section: Enzyme-assisted Signal Generationmentioning

confidence: 99%

Enzyme-Assisted Nucleic Acid Detection for Infectious Disease Diagnostics: Moving toward the Point-of-Care

et al. 2020

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Driven by complex and interconnected factors, including population growth, climate change, and geopolitics, infectious diseases represent one of the greatest healthcare challenges of the 21st century. Diagnostic technologies are the first line of defense in the fight against infectious disease, providing critical information to inform epidemiological models, track diseases, decide treatment choices, and ultimately prevent epidemics. The diagnosis of infectious disease at the genomic level using nucleic acid disease biomarkers has proven to be the most effective approach to date. Such methods rely heavily on enzymes to specifically amplify or detect nucleic acids in complex samples, and significant effort has been exerted to harness the power of enzymes for in vitro nucleic acid diagnostics. Unfortunately, significant challenges limit the potential of enzyme-assisted nucleic acid diagnostics, particularly when translating diagnostic technologies from the lab toward the point-of-use or point-of-care. Herein, we discuss the current state of the field and highlight cross-disciplinary efforts to solve the challenges associated with the successful deployment of this important class of diagnostics at or near the point-of-care.

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Section: Enzyme-assisted Signal Generationmentioning

confidence: 99%

Enzyme-Assisted Nucleic Acid Detection for Infectious Disease Diagnostics: Moving toward the Point-of-Care

et al. 2020

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“…Propanoic acid (0.53%) is an important chemicals, and it is also the intermediate of many other fine chemicals (Bodulev et al, 2018). It is mainly used as food and feed additives, followed by home medicine, rare herbs, medicine, and spices (Belgrano et al, 2018, Nazareth et al, 2018).…”

Section: Results and Analysismentioning

confidence: 99%

Pyrolysis molecule of Torreya grandis bark for potential biomedicine

Chen

Yue

Yang

et al. 2019

Saudi Journal of Biological Sciences

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Torreya grandis is a unique tree species in China. Although full use has been made of the timber, the processing and utilization of the bark has not been effective. In order to explore a new way to utilize the bark of Torreya grandis , a powder of T. grandis bark was prepared and analyzed qualitatively and quantitatively. Differential scanning calorimetry (TG) and pyrolysis gas chromatography-mass spectrometry (PY-GC/MS) revealed many bioactive components in the bark of T. grandis, such as acetic acid, 2-methoxy-4-vinyl phenol, D-mannose, and furfural. These substances have potential broad applications in the chemical industry, biomedicine, and food additives. The chemical constituents of the bark of T. grandis suggest a theoretical basis for the future development and utilization of the bark of T. grandis .

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“…The first system was based on the use of HRP-REP conjugate ( Figure 1 A). The synthesis of HRP-REP conjugate was described previously [ 26 ]. To optimize assay conditions, the concentration of apoHRP-CAP (5:1) varied in the coating solution.…”

Section: Resultsmentioning

confidence: 99%

“…Comparison of solid carrier properties showed that NPs binding surface is higher than that of microtiter plates wells. However, at the use of highly active signaling systems, for example, the enhanced chemiluminescence reaction catalyzed plant peroxidases [ 24 , 25 ], limited surface of the microplates is not an obstacle for the development of sensitive DNA assays [ 26 ]. Also, it should be noted that complicated bioconjugation and separation of NPs, their relatively low yield, poor re-dispersion, high cost and potential environmentally unfriendly impact limit their application [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

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Microplate Chemiluminescent Assay for DNA Detection Using Apoperoxidase-Oligonucleotide as Capture Conjugate and HRP-Streptavidin Signaling System

Sakharov

2018

Sensors

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A covalent conjugate of horseradish apoperoxidase and amino-containing oligonucleotide was synthesized for the first time. Using the obtained conjugate as a capture reagent chemiluminescent microtiter plate-based assay for detection of 35-mer fragment of hepatitis B virus (HBV) DNA (proof-of-concept analyte) was developed. To detect the target DNA, a signaling system consisted of biotinylated reporter oligonucleotide and HRP-streptavidin conjugate was used. The high sensitivity of the assay was due to the enhanced chemiluminescence reaction, where 3-(10′-phenothiazinyl)propane-1-sulfonate/N-morpholinopyridine pair was used as an enhancer. Under the optimized conditions the limit of detection and a working range of the assay were 3 pM and 6–100 pM, respectively. The assay sensitivity was 1.6 × 105 RLU/pM of target. The coefficient of variation (CV) for determination of HBV DNA within the working range was lower than 6%.

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Microplate chemiluminescent assay for HBV DNA detection using 3-(10′-phenothiazinyl)propionic acid/N-morpholinopyridine pair as enhancer of HRP-catalyzed chemiluminescence

Cited by 17 publications

References 45 publications

Enzyme-Assisted Nucleic Acid Detection for Infectious Disease Diagnostics: Moving toward the Point-of-Care

Enzyme-Assisted Nucleic Acid Detection for Infectious Disease Diagnostics: Moving toward the Point-of-Care

Pyrolysis molecule of Torreya grandis bark for potential biomedicine

Microplate Chemiluminescent Assay for DNA Detection Using Apoperoxidase-Oligonucleotide as Capture Conjugate and HRP-Streptavidin Signaling System

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