Micropatterned matrix directs differentiation of human mesenchymal stem cells towards myocardial lineage

Tay, Chor Yong; Yu, Haiyue; Pal, Mintu; Leong, Wen Shing; Tan, Nguan Soon; Ng, Kee Woei; Leong, David Tai; Tan, Lay Poh

doi:10.1016/j.yexcr.2010.02.010

Cited by 156 publications

(193 citation statements)

References 36 publications

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“…Many studies have extensively investigated the modulation of cell shape on hMSC differentiation, such as using strip patterns to guide myogenic differentiation of hMSCs [41,42]. It was an efficient way to induce myogenic differentiation of stem cells via manipulating the cell shape.…”

Section: The Role Of Elongated Fa Versus Elongated Cell Shapementioning

confidence: 99%

Insights into the Role of Focal Adhesion Modulation in Myogenic Differentiation of Human Mesenchymal Stem Cells

Lui

Xiong

et al. 2013

Stem Cells and Development

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We report the establishment of a novel platform to induce myogenic differentiation of human mesenchymal stem cells (hMSCs) via focal adhesion (FA) modulation, giving insights into the role of FA on stem cell differentiation. Micropatterning of collagen type I on a polyacrylamide gel with a stiffness of 10.2 kPa efficiently modulated elongated FA. This elongated FA profile preferentially recruited the b 3 integrin cluster and induced specific myogenic differentiation at both transcription and translation levels with expression of myosin heavy chain and a-sarcomeric actin. This was initiated with elongation of FA complexes that triggered the RhoA downstream signaling toward a myogenic lineage commitment. This study also illustrates how one could partially control myogenic differentiation outcomes of similar-shaped hMSCs by modulating FA morphology and distribution. This technology increases our toolkit choice for controlled differentiation in muscle engineering.

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Section: The Role Of Elongated Fa Versus Elongated Cell Shapementioning

confidence: 99%

Insights into the Role of Focal Adhesion Modulation in Myogenic Differentiation of Human Mesenchymal Stem Cells

Lui

Xiong

et al. 2013

Stem Cells and Development

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“…For example, reduced levels of cell spreading (Watt et al, 1988) and the presence of cell-cell contacts promote the differentiation of epidermal keratinocytes (Charest et al, 2009). Individual elongated hMSCs cultured in nondifferentiating medium on micropatterned lines, reduced their spreading area, had smaller nuclei and adopted myocytes-like phenotypes (Tay et al, 2010). hMSCs treated with transforming growth factor  (TGF-) differentiate into chondrocytes when plated on small micropatterns, but into myocytes when plated on large micropatterns …”

mentioning

confidence: 99%

Micropatterning as a tool to decipher cell morphogenesis and functions

Théry

2010

Journal of Cell Science

646

560

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Cell architectureThe physiological cell microenvironment consists of extracellular matrix (ECM) fibres, adjacent cells and extracellular fluids, and the cell adhesion machinery is the first cellular component to encounter it. Upon binding of extracellular ligands, localised signalling is induced with subsequent assembly of the cytoskeleton. These localised events will affect the entire cell architecture, because the intracellular space is physically connected and SummaryIn situ, cells are highly sensitive to geometrical and mechanical constraints from their microenvironment. These parameters are, however, uncontrolled under classic culture conditions, which are thus highly artefactual. Micro-engineering techniques provide tools to modify the chemical properties of cell culture substrates at sub-cellular scales. These can be used to restrict the location and shape of the substrate regions, in which cells can attach, so-called micropatterns. Recent progress in micropatterning techniques has enabled the control of most of the crucial parameters of the cell microenvironment. Engineered micropatterns can provide a micrometer-scale, soft, 3-dimensional, complex and dynamic microenvironment for individual cells or for multi-cellular arrangements. Although artificial, micropatterned substrates allow the reconstitution of physiological in situ conditions for controlled in vitro cell culture and have been used to reveal fundamental cell morphogenetic processes as highlighted in this review. By manipulating micropattern shapes, cells were shown to precisely adapt their cytoskeleton architecture to the geometry of their microenvironment. Remodelling of actin and microtubule networks participates in the adaptation of the entire cell polarity with respect to external constraints. These modifications further impact cell migration, growth and differentiation. Journal of Cell Sciencemechanically supported by a dynamic equilibrium (Ingber, 2003; Ingber, 2006). Integrin-based cell adhesionIntegrins are transmembrane receptors that bind to ECM proteins and to intracellular actin filaments. When cells contact the ECM, they change their shape and spread in a multi-step process that includes cell attachment, formation of membrane protrusion, extension of cell membrane, and formation and contraction of stress fibers, which further stimulate cell attachment, membrane protrusion and cell shape extension.The attachment of the actin cytoskeleton to cell adhesions requires integrin clustering. Nanopatterning methods have led to determine the maximum distance of 60 nm between integrin molecules -a distance that still allows intracellular recruitment of actin filaments and signalling molecules (Arnold et al., 2004). Arrays of adhesive dots have been used to study the formation of filopodia and subsequent spreading steps. Depending on the cell type and the level of Rac activation, cells need a minimal distance between adhesion sites, so that filopodia can bridge them and promote cell spreading (Guillou et al., 2008;Lehnert et al., 2004). Th...

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“…In another study, derived hMSCs, cultured on PLGA supports, plasma grafted with fibronectin strips, showed highly elongated morphology and markers associated with myogenesis. It was also observed that protein surface micropatterns can highly influence the differentiation process [172].…”

Section: Plasma Grafting and Plasma Treatmentmentioning

confidence: 99%

Strategies for the chemical and biological functionalization of scaffolds for cardiac tissue engineering: a review

Tallawi

Rosellini

Barbani

et al. 2015

J. R. Soc. Interface.

288

208

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The development of biomaterials for cardiac tissue engineering (CTE) is challenging, primarily owing to the requirement of achieving a surface with favourable characteristics that enhances cell attachment and maturation. The biomaterial surface plays a crucial role as it forms the interface between the scaffold (or cardiac patch) and the cells. In the field of CTE, synthetic polymers ( polyglycerol sebacate, polyethylene glycol, polyglycolic acid, poly-L-lactide, polyvinyl alcohol, polycaprolactone, polyurethanes and poly(N-isopropylacrylamide)) have been proven to exhibit suitable biodegradable and mechanical properties. Despite the fact that they show the required biocompatible behaviour, most synthetic polymers exhibit poor cell attachment capability. These synthetic polymers are mostly hydrophobic and lack cell recognition sites, limiting their application. Therefore, biofunctionalization of these biomaterials to enhance cell attachment and cell material interaction is being widely investigated. There are numerous approaches for functionalizing a material, which can be classified as mechanical, physical, chemical and biological. In this review, recent studies reported in the literature to functionalize scaffolds in the context of CTE, are discussed. Surface, morphological, chemical and biological modifications are introduced and the results of novel promising strategies and techniques are discussed.

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Micropatterned matrix directs differentiation of human mesenchymal stem cells towards myocardial lineage

Cited by 156 publications

References 36 publications

Insights into the Role of Focal Adhesion Modulation in Myogenic Differentiation of Human Mesenchymal Stem Cells

Insights into the Role of Focal Adhesion Modulation in Myogenic Differentiation of Human Mesenchymal Stem Cells

Micropatterning as a tool to decipher cell morphogenesis and functions

Strategies for the chemical and biological functionalization of scaffolds for cardiac tissue engineering: a review

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