Micropatterned biofunctional lubricant-infused surfaces promote selective localized cell adhesion and patterning

Imani, Sara M.; Badv, Maryam; Shakeri, Amid; Yousefi, Hanie; Yip, Darren; Fine, Claire; Didar, Tohid F.

doi:10.1039/c9lc00608g

Cited by 39 publications

(33 citation statements)

References 40 publications

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“…Recently, omniphobic coatings have been introduced on the surface of biomedical devices for reducing biofouling and the resultant blood coagulation, [ 20–29 ] while minimizing the administration of anticoagulants. [ 30 ] Liquid‐infused surfaces are one of the recent classes of omniphobic surfaces which have shown to significantly suppress biofouling and thrombosis with their performances surpassing previous anticoagulant based strategies in terms of longevity under blood flow and anti‐biofouling ability.…”

Section: Introductionmentioning

confidence: 99%

“…[ 30 ] Liquid‐infused surfaces are one of the recent classes of omniphobic surfaces which have shown to significantly suppress biofouling and thrombosis with their performances surpassing previous anticoagulant based strategies in terms of longevity under blood flow and anti‐biofouling ability. [ 20–25,27–30 ] However, in order for these surfaces to sustain their omniphobic and repellent properties, the lubricant layer must be stable on the surfaces, making them difficult to use in open‐air applications where the lubricant is susceptible to evaporation. [ 31 ] Another class of omniphobic surfaces is those with structural modifications wherein the micro‐ and nano‐scale topography of the surface provides omniphobic properties.…”

Section: Introductionmentioning

confidence: 99%

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Hierarchical Structures, with Submillimeter Patterns, Micrometer Wrinkles, and Nanoscale Decorations, Suppress Biofouling and Enable Rapid Droplet Digitization

Imani

Maclachlan

Chan

et al. 2020

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agents, [7-12] or changing the surface charge, wettability, chemical affinity, and hydrophilicity. [13-17] Anticoagulants such as heparin have been widely used as coatings on biomedical devices to overcome these adverse effects. [18] Heparin-coated surfaces typically operate through either the release of heparin into the bloodstream for inhibiting clotting in the vicinity of the device surface or reducing coagulation via immobilized heparin on the surface of the device. Anticoagulant coatings fail over time due to leaching and the loss of anticoagulant activity. Furthermore, the administration of anticoagulants (e.g., heparin) both as a coating and a chronic medication, enters the bloodstream, elevating the risk of life-threatening heparin-induced thrombocytopenia, reported to occur in 1-5% of surgical patients. [19] Recently, omniphobic coatings have been introduced on the surface of biomedical devices for reducing biofouling and the resultant blood coagulation, [20-29] while minimizing the administration of anticoagulants. [30] Liquid-infused surfaces are one of the recent classes of omniphobic surfaces which have shown to significantly suppress biofouling and thrombosis with their performances surpassing previous anticoagulant based strategies in terms of longevity under blood flow and anti-biofouling ability. [20-25,27-30] However, in order for these surfaces to sustain their omniphobic and repellent properties, the lubricant layer must be stable on the surfaces, making them difficult to use in open-air applications where the lubricant is susceptible to evaporation. [31] Another class of omniphobic surfaces is those with structural modifications wherein the micro-and nano-scale topography of the surface provides omniphobic properties. Through the formation of micro, nano, and hierarchical structures, air pockets are trapped within the features, leading to the formation of a Cassie wetting state, which reduces the apparent surface energy seen by liquids, [32] resulting in elevated contact angles (CA) and low sliding angles (SA) which lead to omniphobicity. [32] Additionally, the formation of the Cassie state reduces the effective surface area to which platelets and proteins in the blood can bind to, and decreases shear stress at the surfaces reducing platelet adhesion. These two effects reduce the number of nucleation sights for thrombin generation. [33] Hydrophilic polymer surfaces Liquid repellant surfaces have been shown to play a vital role for eliminating thrombosis on medical devices, minimizing blood contamination on common surfaces as well as preventing non-specific adhesion. Herein, an all solution-based, easily scalable method for producing liquid repellant flexible films, fabricated through nanoparticle deposition and heat-induced thin film wrinkling that suppress blood adhesion, and clot formation is reported. Furthermore, superhydrophobic and hydrophilic surfaces are combined onto the same substrate using a facile streamlined process. The patterned superhydrophobic/hydrophilic surfaces show select...

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hierarchical Structures, with Submillimeter Patterns, Micrometer Wrinkles, and Nanoscale Decorations, Suppress Biofouling and Enable Rapid Droplet Digitization

Imani

Maclachlan

Chan

et al. 2020

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“…113,161,162 Our group has shown that the combination of lubricant-infused surfaces with micropatterning strategies in microfluidics with glass substrates can result in the creation of localized bio-interfaces with high sensitivity in capturing secondary antibodies or cells. 163 Integration of this protocol with PDMS microfluidics can bring about flexible biosensors with significantly high sensitivity due to the presence of lubricant.…”

Section: Conclusion and Future Trendsmentioning

confidence: 99%

“…Furthermore, patterning of the biomolecules in PDMS microfluidic channels allows for multiplex detection of different biomarkers for high throughput applications. Incorporating simple methods such as microcontact printing via use of PDMS stamps with microfluidic fabrication 58,163 could significantly simplify the method compared to other patterning techniques such as non-contact printing with piezoelectric inkjet printing technology, 167 photolithographic patterning using a proper photomask, 168 and multifaceted tree-shape designs for the generation of 2 dimensional (2D) concentration gradients. 169 Benefiting from hydrodynamic resistances induced by channel geometries, an innovative, simple microfluidic design has also been demonstrated to generate 1D and 2D concentration gradients with a small footprint size, 117 which could potentially be used in PDMS microfluidic applications.…”

Section: Conclusion and Future Trendsmentioning

confidence: 99%

Conventional and emerging strategies for the fabrication and functionalization of PDMS-based microfluidic devices

2021

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“…[ 31–33 ] Two key aspects that can be modified in ELISA‐IFA to further improve its performance and functionality are the proper immobilization of capture biomolecules and the use of optimal surface blocking agents to reduce the background noise often associated with fluorescence‐based assays. [ 45–47 ] For immobilization of biorecognition elements, contact printing and noncontact printing strategies have been widely used to generate microarrays of biorecognition elements (e.g., antibodies) using desired chemical linkers (e.g., amine or carboxyl groups). [ 48–50 ] The microarrays shape, diameter, thickness, homogeneity, and binding mechanism are important attributes to evaluate the efficacy of different microarray biosensing surface strategies.…”

Section: Introductionmentioning

confidence: 99%

Antibody Micropatterned Lubricant‐Infused Biosensors Enable Sub‐Picogram Immunofluorescence Detection of Interleukin 6 in Human Whole Plasma

Shakeri

Jarad

Terryberry³

et al. 2020

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Recent studies have shown a correlation between elevated interleukin 6 (IL-6) concentrations and the risk of respiratory failure in COVID-19 patients. Therefore, detection of IL-6 at low concentrations permits early diagnosis of worstcase outcome in viral respiratory infections. Here, a versatile biointerface is presented that eliminates nonspecific adhesion and thus enables immunofluorescence detection of IL-6 in whole human plasma or whole human blood during coagulation, down to a limit of detection of 0.5 pg mL −1. The sensitivity of the developed lubricant-infused biosensor for immunofluorescence assays in detecting low molecular weight proteins such as IL-6 is facilitated by i) producing a bioink in which the capture antibody is functionalized by an epoxy-based silane for covalent linkage to the fluorosilanized surface and ii) suppressing nonspecific adhesion by patterning the developed bioink into a lubricant-infused coating. The developed biosensor addresses one of the major challenges for biosensing in complex fluids, namely nonspecific adhesion, therefore paving the way for highly sensitive biosensing in complex fluids. where significantly elevated levels indicate aggressive tumor growth or viral load and poor prognosis in patients. [2,10] Additionally, IL-6 is an important anti-inflammatory cytokine that induces acute responses in chronic inflammatory pathologies. As such, there has been an increasing interest in the use of IL-6 as a biomarker for the diagnosis of early stages of viral infections, cancer, and chronic inflammation. [9-11] A practical IL-6 biosensor should provide a low limit of detection (LOD) (≤5 pg mL −1) and acceptable linear dynamic range (1-100 pg mL −1) in complex fluids, in addition to accuracy, facile operation, and amenable to mass production. [11,12] There are a large number of different IL-6 detection techniques that have been reported in the literature including electrochemical sensors, [13-22] surface plasmon resonance (SPR), [23-25] chemiluminescence immunoassay (CLIA), [26-29] and immunofluorescence assays (IFA), [30-33] Utilizing 0-and 1-D materials such as carbon nanotubes (CNTs), [14,16] nanoparticles and nanowires, [13,19] as well as porous nanoparticles, [15] optical fibers, [32] and microfluidic platforms, [28] have enabled higher sensitivity in IL-6 detection and to date, electrochemical methods have proven to be the most promising candidate for detection of IL-6 at very low concentrations (0.33 pg mL −1 in buffer) with a wide linear dynamic range. [22] While reported IL-6 biosensors have demonstrated satisfactory LODs in buffer or processed serum, their performance in human whole plasma declines significantly, leading to higher LOD's and/or false positive results. In electrochemical sensors, for example, the nonspecific attachment of biological entities in plasma or blood can interfere with the resistivity at the electrodes thereby deteriorating their sensitivity for detection of IL-6 at clinically relevant concentrations. [34] So far, the lowest theoretical LOD fo...

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Micropatterned biofunctional lubricant-infused surfaces promote selective localized cell adhesion and patterning

Abstract: Micropatterned lubricant-infused biofunctional surfaces exhibit biofunctionality and omniphobicity simultaneously which leads to targeted cell attachment and alignment, both in surfaces and in microfluidic devices.

Cited by 39 publications

References 40 publications

Hierarchical Structures, with Submillimeter Patterns, Micrometer Wrinkles, and Nanoscale Decorations, Suppress Biofouling and Enable Rapid Droplet Digitization

Hierarchical Structures, with Submillimeter Patterns, Micrometer Wrinkles, and Nanoscale Decorations, Suppress Biofouling and Enable Rapid Droplet Digitization

Conventional and emerging strategies for the fabrication and functionalization of PDMS-based microfluidic devices

Antibody Micropatterned Lubricant‐Infused Biosensors Enable Sub‐Picogram Immunofluorescence Detection of Interleukin 6 in Human Whole Plasma

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