Microorganisms Isolated from Stored Meat in India, with Potential Antimicrobial Activity against Food Pathogens

Thangavel, Gokila; Thiruvengadam, Subramaniyan

doi:10.2174/1389201020666190314125534

Cited by 7 publications

(4 citation statements)

References 20 publications

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“…Additionally, they have shown antimicrobial activities against pathogenic bacteria, such as Salmonella enterica subsp. enterica serovar Typhimurium and Listeria monocytogenes 46 , 47 . On the other hand, Ln.…”

Section: Discussionmentioning

confidence: 99%

Microbial community structure of plant-based meat alternatives

Roch,

Dzieciol,

Quijada

et al. 2024

npj Sci Food

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A reduction in animal-based diets has driven market demand for alternative meat products, currently raising a new generation of plant-based meat alternatives (PBMAs). It remains unclear whether these substitutes are a short-lived trend or become established in the long term. Over the last few years, the trend of increasing sales and diversifying product range has continued, but publication activities in this field are currently limited mainly to market research and food technology topics. As their popularity increases, questions emerge about the safety and nutritional risks of these novel products. Even though all the examined products must be heated before consumption, consumers lack experience with this type of product and thus further research into product safety, is desirable. To consider these issues, we examined 32 PBMAs from Austrian supermarkets. Based on 16S rRNA gene amplicon sequencing, the majority of the products were dominated by lactic acid bacteria (either Leuconostoc or Latilactobacillus), and generally had low alpha diversity. Pseudomonadota (like Pseudomonas and Shewanella) dominated the other part of the products. In addition to LABs, a high diversity of different Bacillus, but also some Enterobacteriaceae and potentially pathogenic species were isolated with the culturing approach. We assume that especially the dominance of heterofermentative LABs has high relevance for the product stability and quality with the potential to increase shelf life of the products. The number of isolated Enterobacteriaceae and potential pathogens were low, but they still demonstrated that these products are suitable for their presence.

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Section: Discussionmentioning

confidence: 99%

Microbial community structure of plant-based meat alternatives

Roch,

Dzieciol,

Quijada

et al. 2024

npj Sci Food

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“…[29] has been reported in pork meat samples from retail markets. These bacterial species are considered a real danger to human health [30].…”

Section: Prevalence Of Foodborne Bacteria In Raw Pork and Beef Meatmentioning

confidence: 99%

“…2 Percentage in relation to total number of positive samples(25). 3 Percentage in relation to total number of collected samples(30)…”

mentioning

confidence: 99%

Antibiogram of food borne pathogenic bacteria isolated from raw pork and beef meat

Christiana Chika Nwankwo,

Blessing Amaka Ezeonuegbu,

Mabel Doubara Owei

2024

Magna Sci. Adv. Res. Rev.

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Meat is an ideal medium of growth for many organisms because it is high in moisture, rich in nitrogenous compounds (amino acids, peptides, and proteins), minerals, and accessory growth factors. This study isolated foodborne bacteria pathogens from raw pork and beef meat sold in some selected slaughter markets (Alakahia, Rumuosi, and Rumuokoro). We subjected the meat samples to a total viable count (TVC) and a total coliform count (TCC). The highest TVC and TCC for pork meat were log10 7.29 CFU/g and log10 8.66 CFU/g, respectively. For beef meat, TVC and TCC were log10 7.45 CFU/g and log10 6.25 CFU/g, respectively. In this study, most log TVC and TCC counts exceeded the permissible level (log10 ≥6 CFU/g). The most common bacteria found were Escherichia coli, Salmonella enterica, Staphylococcus aureus, and Proteus mirabilis. These bacteria were found in 35.2% of pork meat, 23.6% of beef meat, 35.8% of pork meat, and 7.4% of beef meat, respectively. The rates of antibiotic resistance of the pathogens ranged from 10–87.8% for pork and 14.3–88.5% for beef samples. Ampiclox, amoxicillin, streptomycin, and gentamycin exhibited higher antibiotic resistance. We observed the lowest resistance for ciclopirox, olamine, and cephaloridine. Also, the Multiple Antibiotic Resistance (MAR) index of bacterial isolates ranged from 0.5 to 0.9. This study confirmed an elevated prevalence of Escherichia coli, Salmonella enterica, and Staphylococcus aureus in raw meat products and found the bacterial strains to be resistant to multiple commonly employed antibiotics. Hence, the occurrence and presence of multidrug-resistant foodborne bacterial isolates is an indication that fresh beef meat products may act as reservoirs of drug-resistant bacteria. Careful handling of meat products and effective use of antibiotics are essential to controlling and preventing the spread of these pathogens.

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“…Previously, Leuconostoc spp. have been isolated from chicken and pork samples from Indian (Thangavel et al, 2019) which showed promising results against E. coli , L. monocytogenes , Staphylococcus aureus and S. Typhimurium. The objective of the present study was to evaluate the efficacy of the Leuconostoc isolates in controlling E. coli and L. monocytogenes in spinach stored at 4°C for a period of 5 d.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Efficacy of Leuconostoc spp. Isolated from Indian Meat against Escherichia coli and Listeria monocytogenes in Spinach Leaves

Thangavel

Subramaniyam²

2019

Food Sci Anim Resour

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Five Leuconostoc strains (CM17, CM19, PM30, PM32, and PM36) previously isolated from Indian meat showed promising antimicrobial activity against food pathogens in screening assay. This study evaluates the efficacy of these isolates against Escherichia coli Microbial Type Culture Collection and Gene Bank (MTCC) 443 and Listeria monocytogenes (MTCC 657) in spinach leaves. Challenge studies were conducted by inoculating E. coli and L. monocytogenes at 6 to 7 Log 10 CFU/g of the leaves respectively and treating them with cell free supernatant (CFS) of 48 h cultures of the isolates. The samples were stored at 4°C and analyzed over a period of 5 d. The study was conducted in triplicates and statistical analysis was carried out using one-way Anova. The counts of the pathogens did not increase over the 5 d period in the control samples, without any treatment. Whereas in the case of CFS treatments, significant reduction (p<0.05) was observed in both E. coli and L. monocytogenes from 1 to 5 d with all the 5 strains as compared to the control. The counts of Listeria dropped by 0.5 to 1 log by 5 d, with PM 36 showing the highest reduction (1 log). In the case of E. coli , 1.1 to 1.5 log reduction was observed by 5 d, with again PM 36 showing the highest reduction (1.5). The overall results indicate that the isolates (specifically PM36) not only showed efficacy in in vitro studies but are also proved to be effective in food matrix making them potential clean label antimicrobial alternatives for food application.

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Microorganisms Isolated from Stored Meat in India, with Potential Antimicrobial Activity against Food Pathogens

Cited by 7 publications

References 20 publications

Microbial community structure of plant-based meat alternatives

Microbial community structure of plant-based meat alternatives

Antibiogram of food borne pathogenic bacteria isolated from raw pork and beef meat

Antimicrobial Efficacy of Leuconostoc spp. Isolated from Indian Meat against Escherichia coli and Listeria monocytogenes in Spinach Leaves

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