Microorganisms as Chemical Factories for Isolation of Meta-Bolomes From Mesophilic Soil

Abstract

Objective: To investigate the micro-flora of the mesophilic soil of Rajasthan for isolation of novel compounds having antibacterial potentials. Methods:In present experiments, bacterial colonies were isolated from four different regions of Jaipur, Rajasthan and screened for antimicrobial efficacy against five selected pathogens Pseudomonas aeruginosa MTCC 7093, Staphylococcus aureus MTCC 7443, Escherichia coli MTCC 40, Klebsiella pneumoniae MTCC 530, and Bacillus subtillis MTCC 121. Antimicrobial efficacy against the selected strains was performed. The potential efficacy of the extract was also screened for gas chromatography-mass spectroscopy (GC-MS) analysis for novel metabolites screening. Further, potent bacterial strains were identified at the molecular level by 16S ribosomal deoxyribonucleic acid (DNA) sequencing method. Results:After the primary screening, 29 microbial isolates were selected for the screening of bioactivity. Results displayed zones of inhibition ranging from 5 mm till maximum 13 mm. Soil testing indicated survival conditions for microbes isolated, and biochemical tests supported the identification of screened isolates. The potentially isolated strains S-III C, S-III D and S-IV D were identified at the molecular level using 16S ribosomal DNA sequencing as Bacillus shackletonii, Bacillus thuringiensis and Bacillus subtilis subsp. inaquosorum, respectively. Conclusion:Extraction of active metabolites from soil microbiota, against five pathogenic bacteria, is far better, safe and economical method. This study will help in exploring new compounds against increasing number of resistant pathogenic strains with an aim to reduce demand of medicinal plants for extraction of effective antimicrobial compounds. Bacillus strains (S-IIIC, S-IIID and S-IVC) isolated from soil microflora possess antimicrobial activity and can be used for isolation of antibiotics at industrial levels.

Development and Evaluation of Nanospheres Containing Carbimazole

“…It has observed that while increasing the concentration of polymers (Chitosan) in the formulation (NS4, 5%), the swelling property of nanospheres is also increased as suggested by Sharma R et al, (2012). The swelling aspects of nanospheres reflecting a gel like structure around the nanospheres, controlled the drug release of drug [16].…”

Antibiotic Resistance Profiling of Dairy Wastewater Degrading Native Efficient Microbial Isolates

“…In the previous studies, an optimization model for microbial mediated degradation of oil [4], diesel [5], endosulfan [6], and textile dye [7] has been reported. Today, at global level healthcare is facing the problem of the emergence of multi-resistant bacteria thus the threat of epidemics and pandemics [8] and became a major global health problem [9,10]. Hence, the present study was designed to isolate native DWW degrading microbes under optimized conditions and treatment of DWW under aerobic and anaerobic conditions and determination of antimicrobial resistance profile of efficient strains by disc diffusion method.…”