Microfluidic Platform for Direct Capture and Analysis of Airborne <i>Mycobacterium tuberculosis</i>

Jing, Wenwen; Jiang, Xiran; Zhao, Wang; Liu, Sixiu; Cheng, Xunjia; Sui, Guodong

doi:10.1021/ac500578h

Cited by 59 publications

(46 citation statements)

References 39 publications

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“…SK‐BR‐3 cells are dissociated by using 0.25% trypsin‐EDTA solution. As SK‐BR‐3 cells overexpress HER2 protein on the surface, the SK‐BR‐3 cells can be introduced to microbeads to perform the immunoreaction .…”

Section: Methodsmentioning

confidence: 99%

Ultrafast cell edge detection by line‐scan time‐stretch microscopy

Dai

Zheng

et al. 2018

Journal of Biophotonics

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Ultrafast time-stretch imaging technique recently attracts an increasing interest for applications in cell classification due to high throughput and high sensitivity. A novel imaging modality of time-stretch imaging technique for edge detection is proposed. Edge detection based on the directional derivative is realized using differential detection. As the image processing is mainly implemented in the physical layer, the computation complexity of edge extraction is significantly reduced. An imaging system for edge detection with the scan rate of 77.76 MHz is experimentally demonstrated. Resolution target is first measured to verify the feasibility of the edge extraction. Furthermore, various cells, including red blood cells, lung cancer cells and breast cancer cells, are detected. The edges of cancerous cells present in a completely different form. The imaging system for edge detection would be a good candidate for high-throughput cell classification.

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Section: Methodsmentioning

confidence: 99%

Ultrafast cell edge detection by line‐scan time‐stretch microscopy

Dai

Zheng

et al. 2018

Journal of Biophotonics

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“…While Warkiani et al (2015) reported that shear-modulated inertial microfluidic was potential for malarial detection because its sensitivity is hundred times higher than the standard gold-microscopy method [46]. Direct capture and analysis of infected airborne with Mycobacterium tuberculosis in less than 1 h has been also achieved by using a microfluidic platform [47].…”

Section: Biomedical Assaymentioning

confidence: 99%

Microfluidics Era in Chemistry Field: A Review

et al. 2019

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By miniaturizing the reactor dimension, microfluidic devices are attracting world attention and starting the microfluidic era, especially in the chemistry field because they offer great advantages such as rapid processes, small amount of the required reagents, low risk, ease and accurate control, portable and possibility of online monitoring. Because of that, microfluidic devices have been massively investigated and applied for the real application of human life. This review summarizes the up-to-date microfluidic research works including continuous-flow, droplet-based, open-system, paper-based and digital microfluidic devices. The brief fabrication technique of those microfluidic devices, as well as their potential application for particles separation, solvent extraction, nanoparticle fabrication, qualitative and quantitative analysis, environmental monitoring, drug delivery, biochemical assay and so on, are discussed. Recent perspectives of the microfluidics as a lab-on-chip or micro total analysis system device and organ-on-chip are also introduced.

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“…All the negative samples (1)(2)(3)(4)(5) are detected to be negative using C-mode GLFT and ELISA. For positive samples (6)(7)(8)(9)(10)(11)(12)(13)(14)(15), all the samples are detected to be positive by C-mode GLFT (Figure 7).…”

Section: Serum Samples Analysismentioning

confidence: 99%

A dual-readout chemiluminescent-gold lateral flow test for multiplex and ultrasensitive detection of disease biomarkers in real samples

et al. 2016

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Even though the gold lateral flow test (GLFT) is low-cost and allows for point-of-care testing (POCT), its intrinsic limitations including low sensitivity and incapability of quantification significantly hinder the clinical application of GLFT for assaying disease biomarkers. To improve the performance of the GLFT without sacrificing its simplicity, we develop a chemiluminescent-gold lateral flow test (C-mode GLFT) for quantitative and multiplex detection of disease biomarkers with an ultrahigh sensitivity at a picomolar level. Horseradish peroxidase (HRP) and antibody (Ab) are simultaneously labeled onto the surface of gold nanoparticles (AuNPs) to achieve a dual-readout (chemiluminescent and visual, C&V-mode GLFT). A red color appears at the test line caused by the accumulation of captured AuNPs in the presence of targets, while HRP on the surface of AuNPs catalyzes the chemiluminescence reaction of luminol to amplify the signal. C-mode GLFT is successfully used for detecting tumor biomarkers (alpha fetoprotein, AFP, and carcino embryonic antigen, CEA) and bacterial infection biomarkers (procalcitonin, PCT) in serum samples as well as whole blood. The excellent features of C-mode GLFT such as straightforward operation, ultrahigh sensitivity and quantitative detection, make it a promising platform for POCT of a variety of disease biomarkers in real samples.

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Microfluidic Platform for Direct Capture and Analysis of Airborne Mycobacterium tuberculosis

Cited by 59 publications

References 39 publications

Ultrafast cell edge detection by line‐scan time‐stretch microscopy

Ultrafast cell edge detection by line‐scan time‐stretch microscopy

Microfluidics Era in Chemistry Field: A Review

A dual-readout chemiluminescent-gold lateral flow test for multiplex and ultrasensitive detection of disease biomarkers in real samples

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