Microfluidic immobilized enzyme reactors for continuous biocatalysis

Abstract

This review investigates strategies for employing μ-IMERs for continuous biocatalysis via a top-down approach.

Immobilization of Arabidopsis thaliana Hydroxynitrile Lyase (AtHNL) on EziG Opal

“…Important parameters such as stability, specific rate and productivity were investigated in batch and flow systems. Improved stability, activity and selectivity of immobilized enzymes have been reported earlier [7,8]. In addition, immobilization enables the increase of enzyme loading and facilitates recycling and downstream processing.…”

A chemo-enzymatic tandem reaction in a mixture of deep eutectic solvent and water in continuous flow

“…Furthermore, the combination of both reactions as cascade in flow lower the risks associated to the spontaneous polymerization of the intermediate hydroxystyrene. While conventional flow chemistry, [38][39][40][41][42][43] also including C-C coupling reactions, 39,[44][45][46][47] and continuous biocatalysis [48][49][50] are currently in the focus of many researchers, examples for their successful combination are rare, which can also be attributed by the difficulties to find suitable reaction media. 51 In this work we present a fully integrated two-step flow setup consisting of an enzymatic decarboxylation with a phenolic acid decarboxylase from Bacillus subtilis (BsPAD), followed by a Heck reaction catalyzed by a Pd-substituted Ce-Sn-oxide as shown in Scheme 1.…”

The rise of continuous flow biocatalysis – fundamentals, very recent developments and future perspectives

“…In their recent review, Zhu et al outstandingly focus on microfluidic immobilized enzyme reactors for continuous biocatalysis. 33 Fernandes et al present a perspective on the field of microfluidics when applied to chemical engineering and biotechnology, 34 and Šalić et al focus on microreactors as an effective tool for biotransformations. 35 We would also like to refer to the recently published review article from Žnidaršič-Plazl, which gives a detailed overview of current examples of the implementation of microfluidic devices in biocatalytic process development.…”