Microfluidic fabrication of addressable tethered lipid bilayer arrays and optimization using SPR with silane-derivatized nanoglassy substrates

Taylor, Joseph D.; Phillips, K. Scott; Cheng, Quan

doi:10.1039/b618940g

Cited by 35 publications

(44 citation statements)

References 12 publications

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“…A tethered bilayer membrane (tBLM) was formed on the SPR sensor surface and exposed to solutions with different concentrations of diols. tBLMs were prepared using the vesicle fusion method on a hydrophilic surface (Taylor et al, 2007;Chung et al, 2009). First, polyT-BSA was adsorbed on the SPR sensor surface, and then a suspension of polyA-PEG-DPPE-modified SUVs was applied to the surface.…”

Section: Effects Of Diol Solutions On Tblmmentioning

confidence: 99%

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Highly efficient cryopreservation of human induced pluripotent stem cells using a dimethyl sulfoxide-free solution

et al. 2011

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Section: Effects Of Diol Solutions On Tblmmentioning

confidence: 99%

“…The tBLM was fabricated by the vesicle fusion method using small unilamellar vesicles (SUVs) on a hydrophilic surface (Taylor et al, 2007;Chung et al, 2009). …”

Section: Surface Plasmon Resonance (Spr)mentioning

confidence: 99%

Highly efficient cryopreservation of human induced pluripotent stem cells using a dimethyl sulfoxide-free solution

et al. 2011

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“…Such a construction also allows for the accommodation of proteins [94], incorporated synthetic ion-channels [95] and sugar lectin systems [96]. Taylor et al has reported on SPR detection of cholera toxin using a tethered bilayer [97]. Cornell et al investigated ion channel based sensors with tethered bilayers [98].…”

Section: Tethered Bilayersmentioning

confidence: 99%

Dynamic Nanoplatforms in Biosensor and Membrane Constitutional Systems

Mahon

Aastrup

Barboiu

2011

Constitutional Dynamic Chemistry

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Molecular recognition in biological systems occurs mainly at interfacial environments such as membrane surfaces, enzyme active sites, or the interior of the DNA double helix. At the cell membrane surface, carbohydrate-protein recognition principles apply to a range of specific non-covalent interactions including immune response, cell proliferation, adhesion and death, cell-cell interaction and communication. Protein-protein recognition meanwhile accounts for signalling processes and ion channel structure. In this chapter we aim to describe such constitutional dynamic interfaces for biosensing and membrane transport applications. Constitutionally adaptive interfaces may mimic the recognition capabilities intrinsic to natural recognition processes. We present some recent examples of 2D and 3D constructed sensors and membranes of this type and describe their sensing and transport capabilities.

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“…Such designs are also reminiscent of other on-chip twodimensional assays. 12,[16][17][18] The key to achieving two-dimensional binding assays is to create spatially addressed arrays of bilayers along individual microchannels. There have already been a number of techniques developed to array different bilayer chemistries at each address.…”

Section: Introductionmentioning

confidence: 99%

“…5 In previous work it has been demonstrated that microfluidic devices can be designed for measuring binding affinities in multivalent systems. [5][6][7][8][9][10][11][12][13][14] In our previous setup, ligands were incorporated into supported lipid bilayers (SLBs) coated on the walls and floors of polydimethylsiloxane/glass microchannels. Linear arrays of channels were then monitored by total internal reflection fluorescence microscopy (TIRFM) 15 to obtain equilibrium dissociation constants in one-shot assays.…”

mentioning

confidence: 99%

Multiplexing Ligand−Receptor Binding Measurements by Chemically Patterning Microfluidic Channels

2008

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A method has been designed for patterning supported phospholipid bilayers (SLBs) on planar substrates and inside microfluidic channels. To do this, bovine serum albumin (BSA) monolayers were formed via adsorption at the liquid/solid interface. Next, this interfacial protein film was selectively patterned by using deep UV lithography. Subsequently, SLBs could be deposited in the patterned locations by vesicle fusion. By cycling through this process several times, spatially addressed bilayer arrays could be formed with intervening protein molecules serving as twodimensional corrals. By employing this method, phospholipid bilayers containing various concentrations of ganglioside GM1 were addressed along the length of individual microfluidic channels. Therefore, the binding of GM1 with pentameric cholera toxin B (CTB) subunits could be probed. A seven-channel microfluidic device was fabricated for this purpose. Each channel was simultaneously patterned with four chemically distinct SLBs containing 0, 0.2, 0.5, and 2.0 mol % GM1, respectively. Varying concentrations of CTB were then introduced into each of the channels. With the use of total internal reflection fluorescence microscopy, it was possible to simultaneously abstract multiple equilibrium dissociation constants as a function of ligand density for the CTB-GM1 system in a single shot.Multivalent ligand-receptor interactions are ubiquitous on cell surfaces. They have a wide variety of consequences including the modulation of equilibrium dissociation constants, high receptor selectivity, and receptor clustering. 1 Multivalency can also play a direct role in signal transduction processes. 2 Examination of the underlying thermodynamics of ligandreceptor binding may lead to a greater understanding of its biological role and could provide insight into biomedical applications involving inhibitory drug design. 1,3,4 Unfortunately, high-throughput, low-protein consumption assays are not presently well enough developed in this field to afford rapid, systematic studies of binding data at two-dimensionally fluid bilayer interfaces. 5 In previous work it has been demonstrated that microfluidic devices can be designed for measuring binding affinities in multivalent systems. [5][6][7][8][9][10][11][12][13][14] In our previous setup, ligands were incorporated into supported lipid bilayers (SLBs) coated on the walls and floors of polydimethylsiloxane/glass microchannels. Linear arrays of channels were then monitored by total internal reflection fluorescence microscopy (TIRFM) 15 to obtain equilibrium dissociation constants in one-shot assays. This could be done by using the same surface chemistry in each microchannel while varying the solution concentration of the aqueous proteins. Such a setup made these assays relatively rapid, afforded high accuracy, and used only a few microliters of protein solution. Nevertheless, such platforms could be markedly improved by measuring multiple binding constants with various membrane chemistries simultaneously. This could be achie...

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Microfluidic fabrication of addressable tethered lipid bilayer arrays and optimization using SPR with silane-derivatized nanoglassy substrates

Cited by 35 publications

References 12 publications

Highly efficient cryopreservation of human induced pluripotent stem cells using a dimethyl sulfoxide-free solution

Highly efficient cryopreservation of human induced pluripotent stem cells using a dimethyl sulfoxide-free solution

Dynamic Nanoplatforms in Biosensor and Membrane Constitutional Systems

Multiplexing Ligand−Receptor Binding Measurements by Chemically Patterning Microfluidic Channels

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