Microfluidic device as a new platform for immunofluorescent detection of viruses

Liu, Wen Tso; Zhu, Liang; Qi, Qin; Zhang, Qing; Feng, Han; Ang, Simon S.

doi:10.1039/b509086e

Cited by 66 publications

(49 citation statements)

References 14 publications

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“…Similar microfilter structures can also trap individual microbeads which can act as mobile solid supports for bio-molecules in dynamic microarrays (Tan and Takeuchi 2007). A further application of microfilter structures includes trapping and immunofluorescent identification of microbial cells and viruses (Zhu et al 2004;Liu et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Silicon-based microfilters for whole blood cell separation

Samper

Chen

et al. 2007

Biomed Microdevices

241

176

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This paper reports on the comparison analysis of four main types of silicon-based microfilter for isolation of white blood cells (WBCs) from red blood cells (RBCs) in a given whole blood. The microfilter designs, namely, weir, pillar, crossflow, and membrane, all impose the same cut-off size of 3.5 mum to selectively trap WBCs. Using human whole blood, the microfilters have been characterized and compared for their blood handling capacity, WBCs trapping efficiency and RBCs passing efficiency. Based on the experimental results, the crossflow microfilter is superior and can be integrated with downstream components for on-chip genomic analysis.

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Section: Introductionmentioning

confidence: 99%

Silicon-based microfilters for whole blood cell separation

Samper

Chen

et al. 2007

Biomed Microdevices

241

176

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“…The QDs' inherent characteristics offer several benefits to microfluidic based detection schemes, such as allowing for increased sensitivity and multiplexed detection. Recently, QDs have been used in microchannel assays including antigen-antibody binding for microbe and virus detection (Liu et al 2005b;Lucas et al 2007;Zhang et al 2006c), microbead-based system for signal enhancement and detection of hepatitis and tetanus (Riegger et al 2006). They have also been used for sensing single molecules via detection of a pair of differently colored quantum dots or a paired quantum dot-fluorescent molecule combination bound to the target molecule (Agrawal et al 2006;Stavis et al 2005;Yeh et al 2004).…”

Section: Micro/nanofluidics Integrated With Nanobiosensorsmentioning

confidence: 96%

Applications, techniques, and microfluidic interfacing for nanoscale biosensing

Jungkyu

Junkin

Kim

et al. 2009

Microfluid Nanofluid

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Biosensors based on nanotechnology are rapidly developing and are becoming widespread in the biomedical field and analytical chemistry. For these nanobiosensors to reach their potential, they must be integrated with appropriate packaging techniques, which are usually based on nano/microfluidics. In this review we provide a summary of the latest developments in nanobiosensors with a focus on label-based (fluorescence and nanoparticle) and label-free methods (surface plasmon resonance, micro/nanocantilever, nanowires, and nanopores). An overview on how these sensors interface with nano/microfluidics is then presented and the latest papers in the area summarized.

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“…The bead-based detection can greatly improve the surface to volume ratio for high target capturing efficiency (Lim and Zhang 2007;Liu et al 2005) and the detection sensitivity is increased accordingly (Kuo and Hsieh 2009;Lim and Zhang 2007). With miniaturized beads in channels, the detection spotting region is also much narrowed and real time monitoring can be easily performed.…”

Section: Introductionmentioning

confidence: 98%

Droplet microfluidic preparation of au nanoparticles-coated chitosan microbeads for flow-through surface-enhanced Raman scattering detection

Wang

Yang

Cui

et al. 2010

Microfluid Nanofluid

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An integrated microfluidic device was fabricated to enable on-chip droplet forming, trapping, fusing, shrinking, reaction and producing functional microbeads for a flow-through single bead-based molecule detection. Dielectrophoresis (DEP) force was used to transport target polymer droplets into different predefined microwells, where the droplets were fused through electrocoalescence to form a new one with a desired diameter. In a continuous water loss process with water diffusion to oil phase, the polymer droplet was shrunken and solidified to form a polymer microbead. For a demonstration, Au nanoparticles-coated chitosan microbeads were in situ fabricated through droplet trapping, fusion and shrinking, followed by synthesis of Au nanoparticles on the microbead surface via a photoreduction process. The produced Au nanoparticle/ chitosan microbead embedded in the microwell resulted in a highly sensitive, flow-through surface-enhanced Raman scattering (SERS) detection of Rhodamine 6G (R6G). This work successfully demonstrates an integrated droplet based lab-on-a chip and its application to fabricate an extremely high-throughput single bead based detection platform.

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Microfluidic device as a new platform for immunofluorescent detection of viruses

Cited by 66 publications

References 14 publications

Silicon-based microfilters for whole blood cell separation

Silicon-based microfilters for whole blood cell separation

Applications, techniques, and microfluidic interfacing for nanoscale biosensing

Droplet microfluidic preparation of au nanoparticles-coated chitosan microbeads for flow-through surface-enhanced Raman scattering detection

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