Microdosage des acides α‐aminés et de peptides simples par l'intermédiaire de leurs sels complexes de cuivre

Abstract

For the quantitative serial determination of α‐amino acids and oligopeptides based on the formation of their cupric complexes a cation exchange resin (Dowex 50) containing cupric and phosphoric ions probably as basic cupric phosphate is described as a specific cupric ion donor.

Filter-Paper ionophoresis of cupric complexes of neutral amino acids and oligopeptides

“…Synge, 1956), with the continuous-electrophoresis procedure of Grassmann & Hubner (1953) (see also Pope & Stevens, 1939; Bailey, Chibnall, Rees & Williams, 1943;Schroeder et al 1950;Bennett, 1959). Evidence for formation of 1: 1 complexes of copper with peptides under a wide variety of conditions has been presented by Kober & Sugiura (1912, 1913, Kober & Haw (1916), Kerkkonen (1948, Rauen et al (1949), Woiwod (1949, Spies & Chambers (1951), Baudet & Cherbuliez (1955), Bude6insk? (1957) and Borchers (1959).…”

“…Mixtures of ML+, Land L+could here also bear a spurious resemblance to ML2 in their stoicheiometry. Divergences have been noted by a number of workers, especially at higher phosphate concentrations in the buffer used, which result in lower [Cu2+ ion] (Kober & Sugiura, 1913;Martin & Mittelmann, 1948;Woiwod, 1949;Schroeder, Kay & Mills, 1950;Blackburn & Robson, 1953;Boser, 1954;Spier & Pascher, 1954;Beauchene, Berneking, Schrenk, Mitchell & Silker, 1955;Baudet & Cherbuliez, 1955;Hagan, Ablondi & Hutchings, 1956;Sobel. Henry, Chiamori & Segalove, 1957;Borchers, 1959). A systematic study of the dependence of these divergences on concentration, both for free amino acids and for some oligopeptides, has been made by Rauen, Leonhardi & Buchka (1949).…”