Microdialysis of Excitatory Amino Acids During EEG Recordings in Freely Moving Rats

Soukupová, Marie; Falcicchia, Chiara; Lovisari, Francesca; Ingusci, Selene; Barbieri, Mario; Zucchini, Silvia; Simonato, Michele

doi:10.3791/58455

Cited by 1 publication

(3 citation statements)

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“…Most studies performed in rodent models have not captured such physically coincident analyte and electrophysiological data. The setups that successfully collected neurochemical data and electrophysiology data from the same probe employed small-molecule microdialysis and targeted neurotransmitters such as GABA and glutamate and other amino acids [115,[119][120][121] with improved spatial resolution [115]. These preclinical setups did collect relatively coincident data correlating neurotransmitters to seizure activity but were limited by the lack of sensitivity to large compounds due to the nature of microdialysis.…”

Section: Discussion and Open Challengesmentioning

confidence: 99%

“…Some of the more elementary designs involve two separate probes for neurochemical sampling and EEG recording [95,96,102,104,[106][107][108][109][110][111][112][113][114]. Other designs successfully incorporate both technologies into a single probe [98][99][100][101]103] or even fix separate probes together through various means [115][116][117][118][119][120][121]. The majority of these studies were conducted in vitro or in non-human animal models, with minimal evidence obtained from clinical studies to date [122,123].…”

Section: Previously Fabricated Microdialysis and Microperfusion Electrode Systemsmentioning

confidence: 99%

“…To minimize the spatial gaps between the sEEG probe and the neurochemical sampling probe, several studies have employed a commercially available microdialysis cannula epoxied to an empty guide cannula to permit simultaneous collection of small molecules in proximity to a concentric bipolar electrode in the rodent caudate and nucleus accumbens targeting GABA and other amino acids [115,[119][120][121]. Others have attached recording electrodes directly to the microdialysis cannula to collect zinc ion [116,117] or phenytoin for monitoring seizure activity [118].…”

Section: Preclinical Examples Of Dual-sensing Systems Employing Microdialysis and Microperfusion Electrodesmentioning

confidence: 99%

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Microdialysis and microperfusion electrodes in neurologic disease monitoring

Stangler

Kouzani

Bennet

et al. 2021

Fluids Barriers CNS

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Contemporary biomarker collection techniques in blood and cerebrospinal fluid have to date offered only modest clinical insights into neurologic diseases such as epilepsy and glioma. Conversely, the collection of human electroencephalography (EEG) data has long been the standard of care in these patients, enabling individualized insights for therapy and revealing fundamental principles of human neurophysiology. Increasing interest exists in simultaneously measuring neurochemical biomarkers and electrophysiological data to enhance our understanding of human disease mechanisms. This review compares microdialysis, microperfusion, and implanted EEG probe architectures and performance parameters. Invasive consequences of probe implantation are also investigated along with the functional impact of biofouling. Finally, previously developed microdialysis electrodes and microperfusion electrodes are reviewed in preclinical and clinical settings. Critically, current and precedent microdialysis and microperfusion probes lack the ability to collect neurochemical data that is spatially and temporally coincident with EEG data derived from depth electrodes. This ultimately limits diagnostic and therapeutic progress in epilepsy and glioma research. However, this gap also provides a unique opportunity to create a dual-sensing technology that will provide unprecedented insights into the pathogenic mechanisms of human neurologic disease.

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