Microcontact Printing of Proteins

Abstract

Originally published in: Nanobiotechnology. Edited by Christof M. Niemeyer and Chad A. Mirkin. Copyright © 2004 Wiley‐VCH Verlag GmbH & Co. KGaA Weinheim. Print ISBN: 3‐527‐30658‐9 The sections in this article are Introduction Strategies for Printing Proteins on Surfaces Contact Processing with Hydrogel Stamps Microcontact Printing Affinity‐Contact Printing Microcontact Printing Polypeptides and Proteins Printing One Type of Biomolecule Substrates Resolution and Contrast of the Patterns Activity of Printed Biomolecules Printing Multiple Types of Proteins Additive and Subtractive Printing Parallel Inking and Printing of Multiple Proteins Affinity‐Contact Printing Methods Molds and Stamps Surface Chemistry of Stamps Inking Methods Treatments of Substrates Printing Characterization of the Printed Patterns Conclusion Acknowledgments

Sub-Micrometer Patterning Using Soft Lithography

“…The most compelling application is probably the patterning of bio-macromolecules such as proteins. For example, Delamarche and co-workers have explored the printing of protein features ranging from micrometer lengths scales to the single-molecule level [101]. These authors also developed an elegant extension of the conventional printing scheme by inking a planar stamp with a uniform layer of proteins and removing proteins from unwanted regions upon contact with a sacrificial template (Figure 122.7a) [102].…”