Microcalorimetric study of the adsorption of lactoferrin in supermacroporous continuous cryogel with immobilized Cu2+ ions

Carvalho, Bruna Mara Aparecida de; Silva, L.H.M. Da; Carvalho, L. M.; Soares, Andreimar M.; Minim, Luís Antônio; Silva, S.L. Da

doi:10.1016/j.chroma.2013.08.042

Cited by 20 publications

(10 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Finally, the microcalorimetry has the characteristic of high throughput with simple pretreatment to biosystem, including bacterium, cells, and organelles (Tafin et al 2012). The admirable method has been widely used to evaluate the bioactive fraction of Chinese herbal medicines (CHMs) in recent years (Carvalho et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Antibacterial evaluation of plants extracts against ampicillin-resistant Escherichia coli (E. coli) by microcalorimetry and principal component analysis

Qin

et al. 2019

AMB Expr

View full text Add to dashboard Cite

Antibiotics abuse has caused increased bacterial resistance, which severely limits the application of antibiotics to the treatment of bacterial infections. Therefore, it is urgent to develop new antibacterial drugs through other sources. Dracontomelon dao (Blanco) Merr. & Rolfe (Ren Mianzi in Chinese) is a traditional medicinal material derived from Anacardiaceae with a long history of treating various infectious diseases, such as decubitus and skin ulcers. Recent research has indicated that different extracts from the leaves of D. dao , especially the ethyl acetate (EtOAc) fraction containing flavonoids and phenolic acids, exhibit potent antibacterial activities. In this research, the combined anti-drug-resistant bacterial activities of these active ingredients were investigated. Six samples (S1–S6) were obtained from the EtOAc fraction of D. dao leaves. Microcalorimetric measurements and principal component analysis were performed on the in vitro samples. The results showed that all six samples had notable antibacterial activities. Specifically, sample S6 exhibited a prominent antibacterial effect, with an IC 50 value of 84.3 μg mL −1 , which was significantly lower than that of other samples. The relative contents of main flavonoids and phenolic acids in S6 sample were confirmed by UPLC/Q-TOF-MS. In conclusion, sample S6 from the EtOAc fraction of D. dao leaves could be used as a potential antimicrobial resource in the treatment of infectious diseases. This work provides an insight into the effect of traditional Chinese medicine on drug-resistant bacteria. Moreover, the purification and characterization of the chemical compounds from the sample S6 deserve further analysis.

show abstract

Section: Introductionmentioning

confidence: 99%

Antibacterial evaluation of plants extracts against ampicillin-resistant Escherichia coli (E. coli) by microcalorimetry and principal component analysis

Qin

et al. 2019

AMB Expr

View full text Add to dashboard Cite

show abstract

“…Immobilized metal ion affinity chromatography (IMAC), introduced by Porath and collaborators in 1975, is effective for isolating proteins from non-clarified mixtures (Carvalho et al, 2013). IMAC employs chelated metal ions, such as Cu 2+ , Ni 2+ , Zn 2+ , among others, as a coordination agent for protein links.…”

Section: Introductionmentioning

confidence: 99%

Direct capture of lactoferrin from cheese whey on supermacroporous column of polyacrylamide cryogel with copper ions

Carvalho

Silva

et al. 2014

Food Chemistry

Self Cite

View full text Add to dashboard Cite

Lactoferrin is a protein that is present in cheese whey (a waste product from the dairy industry) and has several biological activities. However, its production from whey must have a high yield and low cost for industrial applications. As such, this study reports the use of polyacrylamide cryogel, loaded with Cu(2+) (through the bond with iminodiacetic acid (IDA)), as an adsorbent for the chromatographic process to capture lactoferrin whey. Ultrafiltered cheese whey was passed through the cryogel-IDA-Cu(2+) system. The eluates were subjected to analysis of total protein, SDS-PAGE and size exclusion chromatography. The results showed an axial dispersion coefficients, at different superficial velocities of liquid, in a range of 10(-6)-10(-5)m(2)/s. The cryogel demonstrated good hydraulic permeability (4.7086×10(-13)m(2)) and a porosity of approximately 78.2%. The IDA-Cu(2+) cryogel system was also able to capture lactoferrin in high purity.

show abstract

“…It is also a universal, non-invasive, and non-destructive method used by many studies in recent years (Braissant et al 2015;Bricheux et al 2013;Carvalho et al 2013;Cirak et al 2003;Guo et al 2012;Kong et al 2012;Li et al 2014;Tim Cushnie and Lamb 2005;Xiao et al 2013;Zhang et al 2014). Among these studies, microcalorimetry has been used to evaluate the antibacterial activity of some compounds or medicinal plants (Bricheux et al 2013;Zhang et al 2014) and the toxicity of some toxic substances or heavy metals (Kong et al 2012;Li et al 2014), as well as monitoring special reactions (Carvalho et al 2013;Xiao et al 2013), etc. All these evaluations are based on the heat changes produced by the metabolic processes during microbial growth.…”

Section: Introductionmentioning

confidence: 98%

Antibacterial evaluation of flavonoid compounds against E. coli by microcalorimetry and chemometrics

Kong

Yan

Xing

et al. 2015

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Fighting against multidrug-resistant bacteria requires reliable methods to evaluate the effect of antibacterial agents. As a universal, non-destructive, and highly sensitive tool, microcalorimetry has been used in many biological investigations to provide continuous real-time monitoring of the metabolic activity. This method, based on heat-flow output, was used to evaluate the influence of two flavonoid compounds (liquiritigenin and liquiritin) on Escherichia coli. Some crucial information, such as the thermogenic power-time curve and thermokinetic parameters of E. coli growth affected by the two compounds, was obtained and further studied by chemometric techniques including similarity analysis, multivariate analysis of variance, and principal component analysis. By comparing the values of two main parameters, k 2 (growth rate constant of the second exponential phase) and Q 1 (heat output of the first exponential growth phase) of E. coli based on the box and whisker plot, liquiritigenin and liquiritin could be differentiated according to their antibacterial effects; liquiritin with IC50 (half-inhibitory concentration) of 198.6 μg mL(-1) expressed a stronger antibacterial effect than liquiritigenin with IC50 of 337.8 μg mL(-1). The glucoside group in liquiritin containing four additional free hydroxyls in the diphenylpropane skeleton was crucial for inducing the antibacterial effect. Liquiritin might be a promising candidate against E. coli. This study provides a valuable method for searching for novel antibacterial agents using microcalorimetry with chemometrics.

show abstract

Microcalorimetric study of the adsorption of lactoferrin in supermacroporous continuous cryogel with immobilized Cu2+ ions

Cited by 20 publications

References 37 publications

Antibacterial evaluation of plants extracts against ampicillin-resistant Escherichia coli (E. coli) by microcalorimetry and principal component analysis

Antibacterial evaluation of plants extracts against ampicillin-resistant Escherichia coli (E. coli) by microcalorimetry and principal component analysis

Direct capture of lactoferrin from cheese whey on supermacroporous column of polyacrylamide cryogel with copper ions

Antibacterial evaluation of flavonoid compounds against E. coli by microcalorimetry and chemometrics

Contact Info

Product

Resources

About