Microbubble-mediated ultrasound promotes accumulation of bone marrow mesenchymal stem cell to the prostate for treating chronic bacterial prostatitis in rats

Yi, Shanhong; Han, Guangwei; Shang, Yangyang; Liu, Chengcheng; Dong, Chao; Yu, Shuangjiang; Liao, Bin; Ao, Xiang; Li, Guangzhi; Li, Longkun

doi:10.1038/srep19745

Cited by 14 publications

(22 citation statements)

References 32 publications

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“…Two articles investigated gene transfection of plasmid DNA into planktonic bacteria (Han et al 2005(Han et al , 2007. The microbubble-mediated accumulation of bone marrow mesenchymal stem cells, which can suppress inflammation, was investigated as a treatment for chronic bacterial prostatitis (Yi et al 2016). Although the majority of sonobactericide studies paired their treatments with therapeutics, 5 (19%) focused directly on the mechanical and biological effects resulting from ultrasound and microbubbles alone (Vollmer et al 1998;Nishikawa et al 2010;Tandiono et al 2012;Agarwal et al 2014;Goh et al 2015).…”

Section: Experimental Approachesmentioning

confidence: 99%

“…Surrounding tissues were evaluated directly after treatment. For chronic bacterial prostatitis modeled in rats, Yi et al (2016) used sonobactericide to induce accumulation of bone marrow mesenchymal stem cells to reduce inflammatory reactions and resolve infection. After 4 wk of infection induction with E. coli, microbubbles (0.1 mL/kg) were directly injected into prostates and insonified (1 MHz, 0.5 MPa, 1% duty cycle) for 5 min.…”

Section: Experimental Approachesmentioning

confidence: 99%

“…Various techniques were used for assessing sonobactericide efficacy. The colony-forming unit plate-counting method was utilized to determine antimicrobial efficacy (14/27, 52%), for some in vitro and all except one in vivo study (Yi et al 2016). Bacterial plating is relatively easy to perform and considered the "gold standard" for determining viable bacteria counts.…”

Section: Experimental Approachesmentioning

confidence: 99%

“…This method can be used for highthroughput screening, but it does not discriminate between live and dead cells. Immunohistochemistry was used by Yi et al (2016) to quantify inflammatory cytokine expression and distribution. Agarwal et al (2014) desiccated their mixed species biofilms to determine fixed biomass for overall density quantification and also, albeit with additional steps, for extracellular protein and polysaccharide content.…”

Section: Experimental Approachesmentioning

confidence: 99%

“…Genetic testing was performed in 7 studies, using the polymerase chain reaction (PCR) method (Han et al 2005(Han et al , 2007Tandiono et al 2012;Zhu et al 2013;Li et al 2015;Yi et al 2016;Dong et al 2017;Zhou et al 2018). PCR was used to investigate the impact of sonobactericide on (i) the expression of genes (Zhu et al 2013;Li et al 2015;Dong et al 2017;Zhou et al 2018) and mRNA (Yi et al 2016); (ii) the successful incorporation of a gene (Han et al 2005(Han et al , 2007; and (iii) the status of genes up-and downstream after incorporation (Han et al 2005). PCR was also used to quantify intracellular DNA released into the supernatant after treatment, which provided an indication of disrupted (lysed) cells (Tandiono et al 2012).…”

Section: Experimental Approachesmentioning

confidence: 99%

See 4 more Smart Citations

Sonobactericide: An Emerging Treatment Strategy for Bacterial Infections

Lattwein

Shekhar

Kouijzer

et al. 2020

Ultrasound in Medicine & Biology

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Ultrasound has been developed as both a diagnostic tool and a potent promoter of beneficial bioeffects for the treatment of chronic bacterial infections. Bacterial infections, especially those involving biofilm on implants, indwelling catheters and heart valves, affect millions of people each year, and many deaths occur as a consequence. Exposure of microbubbles or droplets to ultrasound can directly affect bacteria and enhance the efficacy of antibiotics or other therapeutics, which we have termed sonobactericide. This review summarizes investigations that have provided evidence for ultrasound-activated microbubble or droplet treatment of bacteria and biofilm. In particular, we review the types of bacteria and therapeutics used for treatment and the in vitro and pre-clinical experimental setups employed in sonobactericide research. Mechanisms for ultrasound enhancement of sonobactericide, with a special emphasis on acoustic cavitation and radiation force, are reviewed, and the potential for clinical translation is discussed. (

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Section: Experimental Approachesmentioning

confidence: 99%

Section: Experimental Approachesmentioning

confidence: 99%

Section: Experimental Approachesmentioning

confidence: 99%

Section: Experimental Approachesmentioning

confidence: 99%

Section: Experimental Approachesmentioning

confidence: 99%

See 3 more Smart Citations

Sonobactericide: An Emerging Treatment Strategy for Bacterial Infections

Lattwein

Shekhar

Kouijzer

et al. 2020

Ultrasound in Medicine & Biology

View full text Add to dashboard Cite

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The role of ultrasound in enhancing mesenchymal stromal cell-based therapies

Liu

Ullah

Concepción

et al. 2020

Stem Cells Translational Medicine

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Mesenchymal stromal cells (MSCs) have been a popular platform for cell‐based therapy in regenerative medicine due to their propensity to home to damaged tissue and act as a repository of regenerative molecules that can promote tissue repair and exert immunomodulatory effects. Accordingly, a great deal of research has gone into optimizing MSC homing and increasing their secretion of therapeutic molecules. A variety of methods have been used to these ends, but one emerging technique gaining significant interest is the use of ultrasound. Sound waves exert mechanical pressure on cells, activating mechano‐transduction pathways and altering gene expression. Ultrasound has been applied both to cultured MSCs to modulate self‐renewal and differentiation, and to tissues‐of‐interest to make them a more attractive target for MSC homing. Here, we review the various applications of ultrasound to MSC‐based therapies, including low‐intensity pulsed ultrasound, pulsed focused ultrasound, and extracorporeal shockwave therapy, as well as the use of adjunctive therapies such as microbubbles. At a molecular level, it seems that ultrasound transiently generates a local gradient of cytokines, growth factors, and adhesion molecules that facilitate MSC homing. However, the molecular mechanisms underlying these methods are far from fully elucidated and may differ depending on the ultrasound parameters. We thus put forth minimal criteria for ultrasound parameter reporting, in order to ensure reproducibility of studies in the field. A deeper understanding of these mechanisms will enhance our ability to optimize this promising therapy to assist MSC‐based approaches in regenerative medicine.

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